FastQCFastQC Report
Wed 25 May 2016
SRR2088700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3821387
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT374550.9801415035954223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346880.9077332392662664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270420.7076488196563184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149490.3911930406420496No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG87830.2298380143126043No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78230.20471624569822425No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA73460.19223386691795413No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA68190.1784430626890184No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG64240.16810650164455995No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA62630.16389337169985663No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42830.11207972393269773No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC42730.11181803884296462No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.10794509951491435No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.10192634245105246No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA38840.10163848885234603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110550.022.30711
GTATTGG11450.020.0349331
GGACCCT16000.016.9968766
TTGGACC17450.015.7965624
ATTGGAC14450.015.7474043
TGGACCC17200.015.595935
GACCCTC17000.015.126477
GTATTAG8250.014.5757581
GTATCAA172250.014.316692
TACACCG3250.014.230775
TTAACGG5200.014.23076935
CTAATAC9150.013.9508193
ACGTTTA2051.4279067E-913.53658526
TAGGGGT4900.013.2142854
TAACGGC5650.013.09734536
CCCTCGC19200.012.9114589
TCTAATA9350.012.8609622
GTACTAT4750.012.8526321
TAATACT10550.012.8009484
ATTAGAG8400.012.773813
Produced by FastQC (version 0.11.5)