##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3821387
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5586319836227	31.0	31.0	34.0	30.0	34.0
2	31.76739492754856	31.0	31.0	34.0	30.0	34.0
3	31.84826948958585	31.0	31.0	34.0	30.0	34.0
4	35.5651851016398	37.0	35.0	37.0	33.0	37.0
5	35.43562743056383	37.0	35.0	37.0	33.0	37.0
6	35.47938457947337	37.0	35.0	37.0	33.0	37.0
7	35.34827851772145	37.0	35.0	37.0	33.0	37.0
8	35.373182564341164	37.0	35.0	37.0	33.0	37.0
9	36.97264239397894	39.0	37.0	39.0	33.0	39.0
10	36.78992208849824	39.0	37.0	39.0	32.0	39.0
11	36.87075818282734	39.0	37.0	39.0	33.0	39.0
12	36.738011878932966	39.0	35.0	39.0	32.0	39.0
13	36.78108210448196	39.0	37.0	39.0	32.0	39.0
14	37.784874706487464	40.0	37.0	41.0	32.0	41.0
15	37.79346478124304	40.0	37.0	41.0	32.0	41.0
16	37.684568456426945	39.0	37.0	41.0	32.0	41.0
17	37.69549851925492	39.0	37.0	41.0	32.0	41.0
18	37.65691697805012	39.0	37.0	41.0	32.0	41.0
19	37.704389008493514	40.0	37.0	41.0	32.0	41.0
20	37.57834027278577	39.0	37.0	41.0	32.0	41.0
21	37.52061280367573	39.0	36.0	41.0	32.0	41.0
22	37.39135607045295	39.0	36.0	41.0	31.0	41.0
23	37.227197873442286	39.0	36.0	41.0	31.0	41.0
24	37.07474799071646	39.0	36.0	41.0	31.0	41.0
25	37.25642417268913	39.0	36.0	41.0	31.0	41.0
26	37.151470395434956	39.0	36.0	41.0	31.0	41.0
27	37.04098171684784	39.0	36.0	41.0	31.0	41.0
28	36.882939100384235	39.0	36.0	41.0	30.0	41.0
29	36.780733278257344	39.0	35.0	41.0	30.0	41.0
30	36.60186340718697	39.0	35.0	40.0	30.0	41.0
31	36.48135742336487	39.0	35.0	40.0	30.0	41.0
32	36.314189062767	38.0	35.0	40.0	30.0	41.0
33	36.19322957868439	38.0	35.0	40.0	30.0	41.0
34	36.01899362718301	38.0	35.0	40.0	29.0	41.0
35	35.886948901014215	38.0	35.0	40.0	28.0	41.0
36	35.696336696597335	38.0	35.0	40.0	27.0	41.0
37	35.65332849041461	38.0	35.0	40.0	27.0	41.0
38	35.480418235577815	38.0	34.0	40.0	26.0	41.0
39	35.40600781862711	38.0	34.0	40.0	26.0	41.0
40	35.25988705148157	38.0	34.0	40.0	25.0	41.0
41	35.14504340963111	38.0	34.0	40.0	24.0	41.0
42	34.92065341720166	38.0	34.0	40.0	24.0	41.0
43	34.579138412309455	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	4.0
14	9.0
15	18.0
16	52.0
17	105.0
18	279.0
19	617.0
20	1407.0
21	2739.0
22	4970.0
23	8695.0
24	14308.0
25	22030.0
26	32514.0
27	45324.0
28	61666.0
29	81110.0
30	102572.0
31	127917.0
32	157678.0
33	193610.0
34	237605.0
35	292194.0
36	375641.0
37	513469.0
38	712804.0
39	832044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.977399043855016	21.578002960705106	13.720881972959035	23.723716022480843
2	18.764966751600923	23.3389866035552	34.997397541782604	22.89864910306127
3	19.53233210873434	24.716627758455243	32.400173026181335	23.350867106629085
4	13.726717550460082	16.68896659773009	36.6451238777962	32.939191974013625
5	12.650537618932603	38.51617226938805	35.324608577984904	13.50868153369444
6	32.10135482221508	37.7591434732991	14.153185741198158	15.986315963287675
7	26.01772602460834	32.88779702239004	22.09752636935228	18.996950583649337
8	26.5177538940704	34.87775511875662	19.45204712320422	19.152443863968763
9	24.629303444011295	14.490995023534648	21.86724349038713	39.012458042066925
10	16.052260605900422	27.891862300259042	34.21563950471386	21.84023758912667
11	33.110464865243955	22.72017987186328	23.509291259953518	20.660064002939247
12	20.537202853309545	27.205697826469816	31.412259475420832	20.844839844799807
13	30.65269233396146	21.398356146603316	26.273758716403233	21.675192803031987
14	20.318774308909305	22.94439165674662	28.03518722390588	28.7016468104382
15	22.76631495318323	29.7259345886716	25.407790417458376	22.0999600406868
16	21.737186000789766	28.608800940600887	26.698944650201618	22.955068408407733
17	21.36734646346994	27.435274155692685	27.484026087909964	23.713353292927412
18	22.387578122812478	26.465992583321185	29.392286099261867	21.754143194604474
19	22.467575254743892	25.655344512345913	30.440596568732765	21.43648366417743
20	23.202360818205538	25.37303863754181	30.16216363325672	21.26243691099593
21	22.137276334482742	26.18559700967214	30.61799289106285	21.059133764782263
22	21.268193982970057	26.38110194021176	30.240512149122818	22.110191927695364
23	22.007532866993056	26.486194672248587	30.808394962352676	20.697877498405685
24	21.99047099914246	26.640667380718046	28.929705366140617	22.439156253998878
25	21.910683215282827	26.710877490293445	29.92541713257516	21.453022161848565
26	21.62795864433516	27.2776873946554	29.846937774164196	21.247416186845246
27	20.90165167778087	27.30456245337099	29.53783534617143	22.255950522676716
28	21.21575229098754	26.958588596234822	29.807789684740122	22.017869428037518
29	21.5997751601709	26.89159721326314	29.38181869567254	22.126808930893414
30	21.60377894204382	27.038140863513693	30.42863756013196	20.929442634310526
31	22.04893144818884	26.964685858825604	30.19293779980934	20.793444893176222
32	21.58522546918174	26.788990489578783	29.648240285529837	21.977543755709643
33	21.266519198395766	26.691198771545515	30.21777171482501	21.82451031523371
34	20.84248467899221	26.953328725931186	30.63798563191846	21.566200963158142
35	20.588545467915186	28.3705104978899	29.74433105047984	21.296612983715075
36	22.16417756170731	26.82727501820674	29.529409086282023	21.47913833380393
37	21.39351497244325	27.084302113342616	29.67836023935812	21.843822674856014
38	21.251995675915577	26.140430163184202	30.630213584753392	21.97736057614683
39	20.75181079539968	26.00393522037941	31.32019866085272	21.92405532336819
40	20.039844171762766	26.39151700678314	31.072147364294693	22.496491457159404
41	21.01210895415722	25.510737331759387	31.18896358835156	22.288190125731834
42	20.00692418747434	27.003336746579187	30.593211313065126	22.396527752881347
43	20.30443396599193	26.687456674762334	30.190477959965843	22.817631399279893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1448.0
1	1251.5
2	1055.0
3	1946.5
4	2838.0
5	2838.0
6	4500.0
7	6162.0
8	6644.0
9	7126.0
10	10760.0
11	14394.0
12	14394.0
13	26736.0
14	39078.0
15	59211.0
16	79344.0
17	74345.0
18	69346.0
19	69346.0
20	75896.0
21	82446.0
22	69962.0
23	57478.0
24	65341.5
25	73205.0
26	73205.0
27	82205.5
28	91206.0
29	99458.5
30	107711.0
31	121264.5
32	134818.0
33	134818.0
34	150174.0
35	165530.0
36	178883.5
37	192237.0
38	201275.0
39	210313.0
40	210313.0
41	215909.0
42	221505.0
43	230321.0
44	239137.0
45	247380.5
46	255624.0
47	255624.0
48	308383.5
49	361143.0
50	338731.0
51	316319.0
52	289384.5
53	262450.0
54	262450.0
55	239335.0
56	216220.0
57	188083.5
58	159947.0
59	144838.0
60	129729.0
61	129729.0
62	115008.5
63	100288.0
64	87151.0
65	74014.0
66	63166.5
67	52319.0
68	52319.0
69	44373.5
70	36428.0
71	30456.5
72	24485.0
73	19976.0
74	15467.0
75	15467.0
76	12272.5
77	9078.0
78	7147.0
79	5216.0
80	4110.5
81	3005.0
82	3005.0
83	2326.0
84	1647.0
85	1241.0
86	835.0
87	618.0
88	401.0
89	401.0
90	311.5
91	222.0
92	164.0
93	106.0
94	74.5
95	43.0
96	43.0
97	29.0
98	15.0
99	12.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3821387.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.459719317459886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56614774823886	39.13966043912757
2	12.731500917540744	12.84855925578178
3	4.2698971743729714	6.463734387998256
4	1.815627615876831	3.664642395286951
5	0.9875426689198454	2.4915562943855307
6	0.5741365992887316	1.7382462989994198
7	0.3616981688089692	1.2775831653017866
8	0.25627040380148064	1.0345066116155857
9	0.18969649001469868	0.8614828477484124
>10	1.0595094022285783	10.126995585254074
>50	0.10866667085263622	3.8218905072217924
>100	0.06923486592844512	6.947290780417223
>500	0.005970085189464877	2.0534167573768687
>1k	0.0035820510974756943	3.4022256044904204
>5k	3.1148270412832127E-4	1.1384061990479117
>10k+	2.076551360855475E-4	2.9898028699464856
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37455	0.9801415035954223	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34688	0.9077332392662664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27042	0.7076488196563184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14949	0.3911930406420496	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8783	0.2298380143126043	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7823	0.20471624569822425	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7346	0.19223386691795413	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6819	0.1784430626890184	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6424	0.16810650164455995	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6263	0.16389337169985663	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4283	0.11207972393269773	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4273	0.11181803884296462	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4125	0.10794509951491435	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3895	0.10192634245105246	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3884	0.10163848885234603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.616850897331257E-5
2	0.0	0.0	0.0	0.0	2.616850897331257E-5
3	0.0	0.0	0.0	0.0	2.616850897331257E-5
4	0.0	0.0	0.0	2.616850897331257E-5	2.616850897331257E-5
5	0.0	0.0	0.0	2.616850897331257E-5	2.616850897331257E-5
6	5.233701794662514E-5	0.0	0.0	2.616850897331257E-5	2.616850897331257E-5
7	5.233701794662514E-5	0.0	0.0	2.616850897331257E-5	2.616850897331257E-5
8	5.233701794662514E-5	0.0	0.0	2.616850897331257E-5	2.616850897331257E-5
9	5.233701794662514E-5	0.0	0.0	2.0934807178650056E-4	2.616850897331257E-5
10	5.233701794662514E-5	0.0	0.0	2.878535987064383E-4	2.616850897331257E-5
11	5.233701794662514E-5	0.0	0.0	4.186961435730011E-4	2.616850897331257E-5
12	5.233701794662514E-5	0.0	0.0	6.542127243328143E-4	2.616850897331257E-5
13	5.233701794662514E-5	0.0	0.0	7.588867602260646E-4	2.616850897331257E-5
14	5.233701794662514E-5	0.0	0.0	8.112237781726896E-4	2.616850897331257E-5
15	5.233701794662514E-5	2.616850897331257E-5	0.0	0.0010729088679058154	2.616850897331257E-5
16	5.233701794662514E-5	2.616850897331257E-5	0.0	0.0015177735204521291	2.616850897331257E-5
17	5.233701794662514E-5	2.616850897331257E-5	0.0	0.002119649226838318	2.616850897331257E-5
18	5.233701794662514E-5	2.616850897331257E-5	0.0	0.0026430194063045693	2.616850897331257E-5
19	5.233701794662514E-5	5.233701794662514E-5	0.0	0.0036112542383171346	2.616850897331257E-5
20	5.233701794662514E-5	5.233701794662514E-5	0.0	0.005940251536941953	2.616850897331257E-5
21	5.233701794662514E-5	5.233701794662514E-5	0.0	0.009603842793205713	5.233701794662514E-5
22	5.233701794662514E-5	5.233701794662514E-5	0.0	0.01496838713273479	5.233701794662514E-5
23	5.233701794662514E-5	5.233701794662514E-5	0.0	0.018474967335158674	5.233701794662514E-5
24	5.233701794662514E-5	5.233701794662514E-5	0.0	0.024389050363127315	5.233701794662514E-5
25	5.233701794662514E-5	5.233701794662514E-5	0.0	0.028785359870643828	5.233701794662514E-5
26	5.233701794662514E-5	5.233701794662514E-5	0.0	0.03778732695746335	5.233701794662514E-5
27	5.233701794662514E-5	5.233701794662514E-5	0.0	0.08975798577846211	5.233701794662514E-5
28	5.233701794662514E-5	5.233701794662514E-5	0.0	0.19178900226540782	5.233701794662514E-5
29	5.233701794662514E-5	7.85055269199377E-5	0.0	0.3240969836344762	5.233701794662514E-5
30	5.233701794662514E-5	7.85055269199377E-5	0.0	0.5031680905388541	5.233701794662514E-5
31	5.233701794662514E-5	7.85055269199377E-5	0.0	0.7332939584501649	5.233701794662514E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11055	0.0	22.3071	1
GTATTGG	1145	0.0	20.034933	1
GGACCCT	1600	0.0	16.996876	6
TTGGACC	1745	0.0	15.796562	4
ATTGGAC	1445	0.0	15.747404	3
TGGACCC	1720	0.0	15.59593	5
GACCCTC	1700	0.0	15.12647	7
GTATTAG	825	0.0	14.575758	1
GTATCAA	17225	0.0	14.31669	2
TACACCG	325	0.0	14.23077	5
TTAACGG	520	0.0	14.230769	35
CTAATAC	915	0.0	13.950819	3
ACGTTTA	205	1.4279067E-9	13.536585	26
TAGGGGT	490	0.0	13.214285	4
TAACGGC	565	0.0	13.097345	36
CCCTCGC	1920	0.0	12.911458	9
TCTAATA	935	0.0	12.860962	2
GTACTAT	475	0.0	12.852632	1
TAATACT	1055	0.0	12.800948	4
ATTAGAG	840	0.0	12.77381	3
>>END_MODULE