FastQCFastQC Report
Wed 25 May 2016
SRR2088699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875923
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT185480.9887399429507501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170520.9089925332756195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124580.6640997524951717No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72770.3879157086938004No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39120.20853734401678534No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG36950.1969697050465291No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA32800.1748472618545644No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32680.17420757675021842No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30050.16018781154663597No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG29300.15618977964447367No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.10672079824171887No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA19390.10336245144390256No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.10011071883014389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53100.023.3775881
GTATTGG6450.017.4961221
GTATACT1655.4569682E-1116.8181824
TACCCCG1801.0913936E-1116.4444455
TATACCG1251.6592458E-716.2799995
GTATCAA77400.016.0859182
CTAATAC3450.015.5507243
ATTGGAC6650.015.3007523
TTGGACC9000.014.84
GTTACGC1001.0939488E-414.83
GCGCTTA750.004105691314.818
TGGACCC8850.014.6327685
GACCCTC9450.013.8994717
ACGCGGG800.00630057913.87520
GGACCCT8950.013.8491626
GTATTAG3550.013.5492951
TAGTACA2051.4242687E-913.5365854
CGTTACG1102.457658E-413.4545452
CGCTTAT1102.457658E-413.4545451
TAGACTA1254.8475733E-513.325
Produced by FastQC (version 0.11.5)