##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1875923
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350574090727605	31.0	31.0	34.0	30.0	34.0
2	31.576610553844695	31.0	31.0	34.0	30.0	34.0
3	31.648154535127507	31.0	31.0	34.0	30.0	34.0
4	35.40398086701853	37.0	35.0	37.0	33.0	37.0
5	35.255649618880945	37.0	35.0	37.0	33.0	37.0
6	35.30890873452695	37.0	35.0	37.0	32.0	37.0
7	35.167658267423555	37.0	35.0	37.0	32.0	37.0
8	35.199401041513966	37.0	35.0	37.0	32.0	37.0
9	36.76758587639258	39.0	37.0	39.0	32.0	39.0
10	36.54752727057561	38.0	35.0	39.0	32.0	39.0
11	36.650539494424876	39.0	35.0	39.0	32.0	39.0
12	36.48527791385894	38.0	35.0	39.0	32.0	39.0
13	36.53569362921613	38.0	35.0	39.0	32.0	39.0
14	37.4851009343134	39.0	36.0	41.0	32.0	41.0
15	37.49883923807107	39.0	36.0	41.0	32.0	41.0
16	37.37558098066925	39.0	36.0	41.0	32.0	41.0
17	37.38341605705565	39.0	36.0	41.0	32.0	41.0
18	37.34237226154805	39.0	36.0	41.0	32.0	41.0
19	37.38357811061542	39.0	36.0	41.0	32.0	41.0
20	37.25748284977582	39.0	36.0	40.0	31.0	41.0
21	37.187624971813875	39.0	36.0	40.0	31.0	41.0
22	37.05229319113844	39.0	36.0	40.0	31.0	41.0
23	36.867199773125016	39.0	36.0	40.0	30.0	41.0
24	36.707664973455735	39.0	35.0	40.0	30.0	41.0
25	36.89855233930177	39.0	36.0	40.0	30.0	41.0
26	36.76738117715919	39.0	36.0	40.0	30.0	41.0
27	36.64427111347321	39.0	35.0	40.0	30.0	41.0
28	36.472526857445644	39.0	35.0	40.0	30.0	41.0
29	36.36564400564416	39.0	35.0	40.0	30.0	41.0
30	36.17895937093367	38.0	35.0	40.0	29.0	41.0
31	36.03922229217297	38.0	35.0	40.0	29.0	41.0
32	35.87272185478828	38.0	35.0	40.0	29.0	41.0
33	35.74592080805022	38.0	34.0	40.0	28.0	41.0
34	35.562655290222466	38.0	34.0	40.0	27.0	41.0
35	35.430445172856246	38.0	34.0	40.0	27.0	41.0
36	35.24512146820525	38.0	34.0	40.0	26.0	41.0
37	35.198637683956115	38.0	34.0	40.0	25.0	41.0
38	35.01244827213057	38.0	33.0	40.0	25.0	41.0
39	34.92898215971551	38.0	33.0	40.0	24.0	41.0
40	34.77847971371959	38.0	33.0	40.0	24.0	41.0
41	34.662280381444226	38.0	33.0	40.0	23.0	41.0
42	34.41421902711359	38.0	33.0	40.0	23.0	41.0
43	34.07153918364453	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	4.0
15	5.0
16	31.0
17	71.0
18	197.0
19	412.0
20	895.0
21	1796.0
22	3265.0
23	5551.0
24	8909.0
25	13365.0
26	18983.0
27	26169.0
28	34997.0
29	45307.0
30	56663.0
31	69762.0
32	84069.0
33	103248.0
34	124609.0
35	152247.0
36	193093.0
37	260379.0
38	354154.0
39	317738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.330013545332086	22.166741385440663	14.0657159169113	23.437529152315953
2	19.47121496991081	23.73594225349335	34.186051346457184	22.606791430138657
3	20.159835984739246	24.81386496140833	32.11954861686754	22.906750436984886
4	14.512216119744787	17.091213232099612	36.039432322115566	32.35713832604004
5	12.865186897330009	38.69956282853827	35.021959856561274	13.413290417570444
6	32.57169937145608	37.74787131454756	13.924238894666786	15.756190419329577
7	26.11333194379513	32.95369799293468	21.87312592254586	19.059844140724326
8	26.620442310265403	35.074307420933586	19.282774399588895	19.022475869212116
9	24.845156224429253	14.4268181583146	21.55920045758808	39.16882515966807
10	16.121503921003153	28.121943171441472	34.07618542978577	21.68036747776961
11	33.52381734218302	22.751840027549104	23.15015061918853	20.574192011079344
12	20.50745153185925	27.318125530738733	31.253948056503383	20.920474880898627
13	31.054632839407585	21.308550510868514	26.047977448967792	21.58883920075611
14	20.43777916257757	22.975996349530337	27.746661243558506	28.83956324433359
15	22.934789967392053	30.00656210302875	24.89585126894867	22.162796660630526
16	21.966519947780373	28.512790770196855	26.546185531069238	22.97450375095353
17	21.579030695822805	27.474581845843353	27.357093015011806	23.589294443322036
18	22.602420248592292	26.37299078906757	29.09479760096763	21.92979136137251
19	22.59106583799015	25.670136780667434	30.265261420644663	21.47353596069775
20	23.36114008943864	25.46122628700645	29.89882847003848	21.278805153516426
21	22.40326495277258	26.12767155155089	30.236582205133157	21.232481290543376
22	21.522631792456302	26.400977012382704	29.934277686237653	22.14211350892334
23	22.256243992957067	26.598852937993723	30.31425063821916	20.83065243083005
24	22.12905327137628	26.625133334363937	28.80613969763151	22.439673696628272
25	22.07686562828005	26.73382649501072	29.627655292887823	21.561652583821402
26	21.835277887205393	27.295256788258364	29.506168430154116	21.363296894382124
27	21.13743474545597	27.23032875016725	29.39379708015734	22.23843942421944
28	21.499176671963617	26.93863234258549	29.539591976856194	22.022599008594703
29	21.72354622231296	26.794489965739533	29.12848768312985	22.353476128817654
30	21.81944568087283	27.10105905199734	30.082471402077804	20.997023865052032
31	22.30528651762359	26.91251186749136	29.86620452971684	20.915997085168208
32	21.857453637489385	26.893268007268954	29.283291478381575	21.965986876860082
33	21.628819519777732	26.76266563179832	29.719503412453495	21.889011435970453
34	21.154546321997223	26.92450596319785	30.246497324250516	21.67445039055441
35	20.850589283248834	28.284689723405492	29.48777748340417	21.37694350994151
36	22.35272982952925	26.821676582674236	29.29139415636996	21.534199431426558
37	21.709579764201408	27.04428699898663	29.479781419599842	21.766351817212115
38	21.49219344290784	26.178046753518135	30.388454110323295	21.941305693250733
39	21.06360442299604	26.005918153357037	31.023501497662753	21.906975925984167
40	20.36261616281692	26.292337158827948	30.8157637600264	22.52928291832874
41	21.268410270570808	25.66587221330513	30.77658304738521	22.289134468738855
42	20.335536160066273	26.97743990558248	30.291062053186618	22.395961881164634
43	20.572432877042395	26.667405858342804	29.894617209768203	22.865544054846602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	777.0
1	655.5
2	534.0
3	813.0
4	1092.0
5	1092.0
6	1874.0
7	2656.0
8	2947.5
9	3239.0
10	5039.5
11	6840.0
12	6840.0
13	12959.5
14	19079.0
15	29081.5
16	39084.0
17	36408.5
18	33733.0
19	33733.0
20	37294.5
21	40856.0
22	34752.5
23	28649.0
24	32663.0
25	36677.0
26	36677.0
27	41348.0
28	46019.0
29	50317.5
30	54616.0
31	59811.5
32	65007.0
33	65007.0
34	72122.0
35	79237.0
36	84993.0
37	90749.0
38	95432.5
39	100116.0
40	100116.0
41	102814.0
42	105512.0
43	110533.0
44	115554.0
45	120020.5
46	124487.0
47	124487.0
48	148750.5
49	173014.0
50	163110.0
51	153206.0
52	140452.5
53	127699.0
54	127699.0
55	117018.0
56	106337.0
57	94173.5
58	82010.0
59	74355.0
60	66700.0
61	66700.0
62	59377.0
63	52054.0
64	45625.0
65	39196.0
66	33492.0
67	27788.0
68	27788.0
69	23682.5
70	19577.0
71	16521.0
72	13465.0
73	11093.0
74	8721.0
75	8721.0
76	7052.5
77	5384.0
78	4175.5
79	2967.0
80	2314.5
81	1662.0
82	1662.0
83	1245.0
84	828.0
85	621.0
86	414.0
87	311.5
88	209.0
89	209.0
90	161.5
91	114.0
92	78.0
93	42.0
94	25.5
95	9.0
96	9.0
97	9.0
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1875923.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.68321778631653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7305654003378	48.54895786973126
2	10.42636365419213	12.237051380765838
3	2.9565662797589134	5.20502468684316
4	1.2121050653279655	2.8452090211415375
5	0.6166784911101133	1.8094339098975927
6	0.3847080222713956	1.354554279305725
7	0.2604729838984047	1.0699774989093263
8	0.194529316885961	0.9132485014913786
9	0.15475219600932683	0.8173221139193465
>10	0.9354338399864669	10.543387455808158
>50	0.07903211452290443	3.1968983319685957
>100	0.04278537783554997	4.6191978729215855
>500	0.0035497432826546065	1.4818344271591257
>1k	0.0020934383461809216	2.402299145582988
>5k	9.101905852960529E-5	0.3886857631271657
>10k+	2.730571755888159E-4	2.5669177414271447
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18548	0.9887399429507501	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17052	0.9089925332756195	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12458	0.6640997524951717	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7277	0.3879157086938004	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3912	0.20853734401678534	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3695	0.1969697050465291	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3280	0.1748472618545644	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3268	0.17420757675021842	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3005	0.16018781154663597	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2930	0.15618977964447367	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2002	0.10672079824171887	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1939	0.10336245144390256	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1878	0.10011071883014389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.330709202883061E-5	0.0	0.0	0.0	0.0
9	5.330709202883061E-5	5.330709202883061E-5	0.0	5.330709202883061E-5	0.0
10	5.330709202883061E-5	5.330709202883061E-5	0.0	5.330709202883061E-5	0.0
11	5.330709202883061E-5	1.0661418405766122E-4	0.0	2.1322836811532244E-4	0.0
12	5.330709202883061E-5	1.0661418405766122E-4	0.0	4.7976382825947546E-4	0.0
13	1.5992127608649182E-4	1.0661418405766122E-4	0.0	5.33070920288306E-4	0.0
14	1.5992127608649182E-4	1.0661418405766122E-4	0.0	6.396851043459673E-4	0.0
15	1.5992127608649182E-4	1.0661418405766122E-4	0.0	0.0010128347485477815	0.0
16	1.5992127608649182E-4	1.0661418405766122E-4	0.0	0.0012793702086919346	0.0
17	1.5992127608649182E-4	1.0661418405766122E-4	0.0	0.0017591340369514101	0.0
18	1.5992127608649182E-4	1.5992127608649182E-4	0.0	0.0019190553130379018	0.0
19	1.5992127608649182E-4	1.5992127608649182E-4	0.0	0.0026653546014415302	0.0
20	1.5992127608649182E-4	1.5992127608649182E-4	0.0	0.004531102822450602	0.0
21	1.5992127608649182E-4	1.5992127608649182E-4	0.0	0.00788944962026693	0.0
22	1.5992127608649182E-4	2.1322836811532244E-4	0.0	0.01322015882314999	0.0
23	1.5992127608649182E-4	2.1322836811532244E-4	0.0	0.016258663068793335	0.0
24	1.5992127608649182E-4	2.1322836811532244E-4	0.0	0.022495592836166516	0.0
25	1.5992127608649182E-4	2.1322836811532244E-4	0.0	0.02617378218615583	0.0
26	1.5992127608649182E-4	2.1322836811532244E-4	0.0	0.034489688542653406	0.0
27	1.5992127608649182E-4	2.66535460144153E-4	0.0	0.08241276427657213	0.0
28	1.5992127608649182E-4	3.7314964420181426E-4	0.0	0.17612663206325632	0.0
29	1.5992127608649182E-4	4.264567362306449E-4	0.0	0.29649404586435585	0.0
30	1.5992127608649182E-4	4.264567362306449E-4	0.0	0.466063905608066	0.0
31	1.5992127608649182E-4	4.264567362306449E-4	0.0	0.6832369985335219	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5310	0.0	23.377588	1
GTATTGG	645	0.0	17.496122	1
GTATACT	165	5.4569682E-11	16.818182	4
TACCCCG	180	1.0913936E-11	16.444445	5
TATACCG	125	1.6592458E-7	16.279999	5
GTATCAA	7740	0.0	16.085918	2
CTAATAC	345	0.0	15.550724	3
ATTGGAC	665	0.0	15.300752	3
TTGGACC	900	0.0	14.8	4
GTTACGC	100	1.0939488E-4	14.8	3
GCGCTTA	75	0.0041056913	14.8	18
TGGACCC	885	0.0	14.632768	5
GACCCTC	945	0.0	13.899471	7
ACGCGGG	80	0.006300579	13.875	20
GGACCCT	895	0.0	13.849162	6
GTATTAG	355	0.0	13.549295	1
TAGTACA	205	1.4242687E-9	13.536585	4
CGTTACG	110	2.457658E-4	13.454545	2
CGCTTAT	110	2.457658E-4	13.454545	1
TAGACTA	125	4.8475733E-5	13.32	5
>>END_MODULE