FastQCFastQC Report
Wed 25 May 2016
SRR2088698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803515
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT411561.0820517337252515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT376160.9889799304064792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT296590.7797787046981542No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166090.4366750229721718No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG89410.23507203205456007No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG79060.20786036074525802No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75270.19789589366677926No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA72850.19153335795967677No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG66650.1752326466439596No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59560.1565919945103411No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA45870.12059897226644302No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.11920552436364784No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43590.11460451713743736No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.1115547066332064No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39860.10479779887814299No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39160.1029573959876588No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38310.10072262104921369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124200.022.4174731
GTATTGG12650.018.2806321
ATTGGAC14500.015.94827653
TAGTACT4650.015.1182794
GTATCAA186250.014.9787922
GCGCTAA750.004106723214.811
GTATTAG8450.014.230771
CCGTTTA2101.364242E-1014.09523827
TTGGACC18700.014.0481284
TTTAGCG800.0063021513.87526
CGCGATA1652.5997178E-713.45454514
ATTAGAG8000.013.41253
GACCCTC18550.013.164427
GGACCCT19400.013.1597946
TAGGACG1703.7378413E-713.0588244
TGGACCC19150.013.0417755
TATACTG4900.012.8367345
TACCCCG5250.012.6857155
TGCGACG2358.112693E-1012.59574422
CTAATAC9600.012.5260423
Produced by FastQC (version 0.11.5)