##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3803515
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.574915045688	31.0	31.0	34.0	30.0	34.0
2	31.779692468677	31.0	31.0	34.0	30.0	34.0
3	31.856192495625756	31.0	31.0	34.0	30.0	34.0
4	35.57105940163244	37.0	35.0	37.0	33.0	37.0
5	35.44876541830386	37.0	35.0	37.0	33.0	37.0
6	35.49629881832989	37.0	35.0	37.0	33.0	37.0
7	35.35727294357982	37.0	35.0	37.0	33.0	37.0
8	35.38912321891724	37.0	35.0	37.0	33.0	37.0
9	36.98629609716276	39.0	37.0	39.0	33.0	39.0
10	36.79738136960154	39.0	37.0	39.0	32.0	39.0
11	36.891339721284126	39.0	37.0	39.0	33.0	39.0
12	36.745660001340866	39.0	37.0	39.0	32.0	39.0
13	36.787255472898096	39.0	37.0	39.0	32.0	39.0
14	37.78945081063174	40.0	37.0	41.0	32.0	41.0
15	37.797515719012544	40.0	37.0	41.0	32.0	41.0
16	37.690651147688385	39.0	37.0	41.0	32.0	41.0
17	37.71146137191519	39.0	37.0	41.0	32.0	41.0
18	37.67611275359766	39.0	37.0	41.0	32.0	41.0
19	37.72063262534787	40.0	37.0	41.0	32.0	41.0
20	37.601565131201006	39.0	37.0	41.0	32.0	41.0
21	37.54149911332018	39.0	36.0	41.0	32.0	41.0
22	37.4117388783796	39.0	36.0	41.0	32.0	41.0
23	37.23661902214136	39.0	36.0	41.0	31.0	41.0
24	37.08863169988813	39.0	36.0	41.0	31.0	41.0
25	37.26149495926794	39.0	36.0	41.0	31.0	41.0
26	37.1371633870249	39.0	36.0	41.0	31.0	41.0
27	37.03157921028312	39.0	36.0	41.0	31.0	41.0
28	36.872098309064114	39.0	35.0	41.0	30.0	41.0
29	36.761089676259985	39.0	35.0	41.0	30.0	41.0
30	36.55985134802939	39.0	35.0	40.0	30.0	41.0
31	36.421621841901505	39.0	35.0	40.0	30.0	41.0
32	36.246035312073175	38.0	35.0	40.0	30.0	41.0
33	36.116978636866165	38.0	35.0	40.0	30.0	41.0
34	35.941065041152726	38.0	35.0	40.0	29.0	41.0
35	35.8092446066336	38.0	35.0	40.0	28.0	41.0
36	35.62007958427928	38.0	35.0	40.0	27.0	41.0
37	35.55484597799667	38.0	34.0	40.0	26.0	41.0
38	35.37277833793215	38.0	34.0	40.0	26.0	41.0
39	35.28450972324284	38.0	34.0	40.0	25.0	41.0
40	35.12514056077076	38.0	34.0	40.0	24.0	41.0
41	35.00063520191192	38.0	34.0	40.0	24.0	41.0
42	34.74489754871481	38.0	33.0	40.0	23.0	41.0
43	34.39577943034272	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	1.0
13	5.0
14	10.0
15	20.0
16	48.0
17	126.0
18	271.0
19	603.0
20	1411.0
21	2849.0
22	5020.0
23	8951.0
24	14839.0
25	22673.0
26	32774.0
27	45799.0
28	62108.0
29	81468.0
30	103101.0
31	127985.0
32	157035.0
33	193077.0
34	237392.0
35	293149.0
36	376283.0
37	519160.0
38	704090.0
39	813262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19499988826125	21.427679396558183	13.864491135173648	23.512829580006915
2	18.902778088163185	23.331839101462727	34.85184099444856	22.91354181592553
3	19.654240879817745	24.62966492836232	32.431658610522106	23.284435581297828
4	13.764820172918999	16.565256085489345	36.37374901899953	33.296174722592134
5	12.785778418121133	38.564538328362055	34.953773023111516	13.695910230405294
6	32.33409096585658	37.43976295610771	13.910369750086431	16.315776327949276
7	26.07701034437882	32.978074228706866	21.84287428865142	19.102041138262894
8	26.76001014850737	34.70855248368943	19.160197869602197	19.371239498201007
9	24.667682393785746	14.474900191007528	21.778276147195424	39.079141268011305
10	16.29603143408137	27.913443222913543	33.82855069586948	21.96197464713561
11	33.10942641214771	22.809375012324125	23.341488070902834	20.73971050462533
12	20.786903693031313	27.358193670854458	31.055668243716667	20.79923439239756
13	30.90031194828994	21.274610459009626	26.217643416681675	21.60743417601876
14	20.46146787905398	23.07213196214554	27.896406350441634	28.569993808358845
15	22.855148461357455	29.66692651402716	25.424350896473392	22.053574128141996
16	21.827493778780944	28.409773591007266	26.823951003216763	22.93878162699503
17	21.53368660305007	27.305505565246886	27.365502699476668	23.795305132226375
18	22.489591864367565	26.169819232998947	29.49153611856401	21.84905278406947
19	22.607193609069505	25.385255480785535	30.63852778285349	21.369023127291467
20	23.253280189508914	25.177473994449873	30.32521233648349	21.244033479557725
21	22.142465587752383	26.00857364832267	30.76590995434486	21.083050809580087
22	21.235856832430002	26.270410396698846	30.3355185926702	22.158214178200954
23	21.993655868321802	26.42697609973932	30.945506984986253	20.63386104695262
24	21.98781916201198	26.52896071134201	29.036693689915776	22.446526436730235
25	21.95087964685298	26.597397407398155	30.04016022021735	21.411562725531518
26	21.592553204075703	27.264596038138407	29.994044982075792	21.148805775710102
27	20.87831913374865	27.228103477967093	29.678783966935846	22.21479342134841
28	21.18240627419637	26.930352581756612	29.922321852286636	21.964919291760385
29	21.56539411570613	26.803548822602252	29.442528818737408	22.188528242954213
30	21.512706010098555	27.07464016837057	30.659534667274873	20.753119154255998
31	22.12424559913659	26.813933953198553	30.31661502583794	20.745205421826913
32	21.57919713738476	26.739949757001092	29.715434275926345	21.965418829687803
33	21.285442544593618	26.706980253791556	30.19759354176334	21.80998365985148
34	20.79684186863993	26.85547447558377	30.773034942678024	21.57464871309828
35	20.502824361150147	28.41466380440198	29.812239467965817	21.270272366482057
36	22.246474642534604	26.704482564154475	29.615552981912785	21.43348981139814
37	21.394815059228108	27.059732904957652	29.749034774412614	21.796417261401622
38	21.16137309830512	26.15656833218746	30.81302426834126	21.869034301166156
39	20.697092031975686	25.95315122984923	31.467392661787848	21.882364076387237
40	19.984225118081564	26.38306934506634	31.16919481058968	22.463510726262417
41	20.954958768402385	25.44793434494145	31.244230665581707	22.352876221074453
42	19.886762639295494	27.093412277853513	30.65469703681989	22.365128046031106
43	20.27624447386168	26.745470965672542	30.155080234993157	22.82320432547262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1273.0
1	1199.0
2	1125.0
3	2128.0
4	3131.0
5	3131.0
6	4786.5
7	6442.0
8	7060.5
9	7679.0
10	11822.5
11	15966.0
12	15966.0
13	29721.0
14	43476.0
15	65105.5
16	86735.0
17	80485.5
18	74236.0
19	74236.0
20	80401.5
21	86567.0
22	71283.5
23	56000.0
24	63019.0
25	70038.0
26	70038.0
27	78288.5
28	86539.0
29	94176.5
30	101814.0
31	113168.5
32	124523.0
33	124523.0
34	139035.0
35	153547.0
36	167816.0
37	182085.0
38	192085.5
39	202086.0
40	202086.0
41	207573.5
42	213061.0
43	224822.5
44	236584.0
45	246225.0
46	255866.0
47	255866.0
48	311883.0
49	367900.0
50	346556.0
51	325212.0
52	296753.0
53	268294.0
54	268294.0
55	242841.5
56	217389.0
57	189135.5
58	160882.0
59	144909.5
60	128937.0
61	128937.0
62	114150.5
63	99364.0
64	86629.5
65	73895.0
66	63079.0
67	52263.0
68	52263.0
69	44509.5
70	36756.0
71	31066.0
72	25376.0
73	20905.5
74	16435.0
75	16435.0
76	13242.0
77	10049.0
78	7799.0
79	5549.0
80	4384.0
81	3219.0
82	3219.0
83	2447.5
84	1676.0
85	1226.0
86	776.0
87	588.0
88	400.0
89	400.0
90	309.5
91	219.0
92	150.0
93	81.0
94	65.0
95	49.0
96	49.0
97	31.0
98	13.0
99	10.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3803515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.04140577791984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.31067917250955	35.13450944970169
2	12.894152070950849	11.87329775321707
3	4.416974461927238	6.100911405369034
4	2.0242701020354104	3.728009646876941
5	1.0913878976156433	2.512451652761633
6	0.649960097790555	1.7955045961098843
7	0.45456384406345623	1.465013087754767
8	0.3054087634456733	1.124915904474798
9	0.23211631878943592	0.9618265458955275
>10	1.3730371502512275	12.127320261868903
>50	0.1440196062143923	4.603755622864804
>100	0.09095399089118769	8.026748863641139
>500	0.0076690557446532906	2.3572193701224426
>1k	0.004235150179057976	3.726881946778491
>5k	3.433905550587548E-4	1.1667915707694758
>10k+	2.2892703670583656E-4	3.2948423217934795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41156	1.0820517337252515	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37616	0.9889799304064792	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29659	0.7797787046981542	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16609	0.4366750229721718	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8941	0.23507203205456007	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7906	0.20786036074525802	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7527	0.19789589366677926	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7285	0.19153335795967677	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6665	0.1752326466439596	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5956	0.1565919945103411	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4587	0.12059897226644302	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4534	0.11920552436364784	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4359	0.11460451713743736	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4243	0.1115547066332064	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3986	0.10479779887814299	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3916	0.1029573959876588	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3831	0.10072262104921369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.6291469864059955E-5	0.0
3	0.0	0.0	0.0	2.6291469864059955E-5	0.0
4	0.0	0.0	0.0	7.887440959217987E-5	0.0
5	0.0	0.0	0.0	7.887440959217987E-5	0.0
6	0.0	0.0	0.0	7.887440959217987E-5	0.0
7	0.0	0.0	0.0	7.887440959217987E-5	0.0
8	0.0	0.0	0.0	7.887440959217987E-5	0.0
9	0.0	0.0	0.0	2.892061685046595E-4	0.0
10	0.0	0.0	0.0	3.6808057809683935E-4	0.0
11	0.0	0.0	0.0	6.572867466014989E-4	0.0
12	0.0	0.0	0.0	8.150355657858586E-4	0.0
13	0.0	0.0	0.0	8.676185055139786E-4	0.0
14	0.0	0.0	0.0	8.676185055139786E-4	0.0
15	0.0	0.0	0.0	9.727843849702183E-4	0.0
16	0.0	0.0	0.0	0.0012882820233389377	0.0
17	0.0	0.0	0.0	0.0019192773000763768	0.0
18	0.0	0.0	0.0	0.002261066408309156	0.0
19	0.0	0.0	0.0	0.0030761019740950146	0.0
20	0.0	0.0	0.0	0.004995379274171392	0.0
21	0.0	0.0	0.0	0.008413270356499185	0.0
22	0.0	0.0	0.0	0.013066860522437797	0.0
23	0.0	0.0	0.0	0.01635329425544529	0.0
24	5.258293972811991E-5	0.0	0.0	0.02221629203513066	0.0
25	5.258293972811991E-5	0.0	0.0	0.02729054571889423	0.0
26	5.258293972811991E-5	0.0	0.0	0.03641368576172304	0.0
27	5.258293972811991E-5	0.0	0.0	0.09128398336801616	0.0
28	5.258293972811991E-5	0.0	0.0	0.1992630500997104	0.0
29	5.258293972811991E-5	0.0	0.0	0.34236752056978875	0.0
30	5.258293972811991E-5	0.0	0.0	0.5335590894212328	0.0
31	5.258293972811991E-5	0.0	0.0	0.7816453990585025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12420	0.0	22.417473	1
GTATTGG	1265	0.0	18.280632	1
ATTGGAC	1450	0.0	15.9482765	3
TAGTACT	465	0.0	15.118279	4
GTATCAA	18625	0.0	14.978792	2
GCGCTAA	75	0.0041067232	14.8	11
GTATTAG	845	0.0	14.23077	1
CCGTTTA	210	1.364242E-10	14.095238	27
TTGGACC	1870	0.0	14.048128	4
TTTAGCG	80	0.00630215	13.875	26
CGCGATA	165	2.5997178E-7	13.454545	14
ATTAGAG	800	0.0	13.4125	3
GACCCTC	1855	0.0	13.16442	7
GGACCCT	1940	0.0	13.159794	6
TAGGACG	170	3.7378413E-7	13.058824	4
TGGACCC	1915	0.0	13.041775	5
TATACTG	490	0.0	12.836734	5
TACCCCG	525	0.0	12.685715	5
TGCGACG	235	8.112693E-10	12.595744	22
CTAATAC	960	0.0	12.526042	3
>>END_MODULE