FastQCFastQC Report
Wed 25 May 2016
SRR2088697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731713
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT276001.0103550409578166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT252860.9256462886108461No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198300.7259181326881704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110070.4029339831819814No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55400.2028031495255907No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG53300.19511566551830298No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48850.17882552083619327No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA47970.1756040989664727No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA44650.1634505528216178No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG42380.15514074868040675No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.11344529970754615No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.1062703146340776No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.10110871822918441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84100.022.4595721
TAATACT6550.017.7938924
GTATTGG8000.016.418751
CGCGATA803.3846998E-416.187514
GTATTAG5900.014.7372881
GTATCAA127650.014.7246382
CTAATAC6500.014.5153843
ATTAGAC2700.014.3888883
TAGTACT3750.013.8133334
CGAACTA3500.013.74285724
TAACGGC3100.013.72580736
TATACCG1356.574426E-613.7037035
TACCCCG3000.013.5666675
TTAACGG3150.013.50793735
GGACCCT11800.013.1694926
TGGACCC11950.013.1589975
TTGGACC12950.013.1428574
TACTGGT6350.013.1102357
GACGTAT1703.7365498E-713.05882328
CGTCTAA850.00940906613.0588231
Produced by FastQC (version 0.11.5)