##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2731713
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583146911846157	31.0	31.0	34.0	30.0	34.0
2	31.782979031838263	31.0	31.0	34.0	30.0	34.0
3	31.861710582334236	31.0	31.0	34.0	30.0	34.0
4	35.57597631962069	37.0	35.0	37.0	33.0	37.0
5	35.45089619590345	37.0	35.0	37.0	33.0	37.0
6	35.49764305401043	37.0	35.0	37.0	33.0	37.0
7	35.36095336515952	37.0	35.0	37.0	33.0	37.0
8	35.39015189370186	37.0	35.0	37.0	33.0	37.0
9	36.993331290659015	39.0	37.0	39.0	33.0	39.0
10	36.8119235073377	39.0	37.0	39.0	32.0	39.0
11	36.89460239783608	39.0	37.0	39.0	33.0	39.0
12	36.75919395631971	39.0	37.0	39.0	32.0	39.0
13	36.798557901214366	39.0	37.0	39.0	32.0	39.0
14	37.80432095172516	40.0	37.0	41.0	32.0	41.0
15	37.81836305644114	40.0	37.0	41.0	32.0	41.0
16	37.712185723756484	40.0	37.0	41.0	32.0	41.0
17	37.727327504756175	39.0	37.0	41.0	32.0	41.0
18	37.685529555996546	39.0	37.0	41.0	32.0	41.0
19	37.726770345200975	40.0	37.0	41.0	32.0	41.0
20	37.60435082309159	39.0	37.0	41.0	32.0	41.0
21	37.54470034004304	39.0	36.0	41.0	32.0	41.0
22	37.40870801581279	39.0	36.0	41.0	32.0	41.0
23	37.23725698856359	39.0	36.0	41.0	31.0	41.0
24	37.08777129954721	39.0	36.0	41.0	31.0	41.0
25	37.26447251230272	39.0	36.0	41.0	31.0	41.0
26	37.14910424338135	39.0	36.0	41.0	31.0	41.0
27	37.03609566597955	39.0	36.0	41.0	31.0	41.0
28	36.874458993312984	39.0	35.0	41.0	30.0	41.0
29	36.76695245803641	39.0	35.0	41.0	30.0	41.0
30	36.5820999497385	39.0	35.0	40.0	30.0	41.0
31	36.44405287085429	39.0	35.0	40.0	30.0	41.0
32	36.26977138520774	38.0	35.0	40.0	30.0	41.0
33	36.14423806600474	38.0	35.0	40.0	30.0	41.0
34	35.96363929885753	38.0	35.0	40.0	29.0	41.0
35	35.831054726466505	38.0	35.0	40.0	28.0	41.0
36	35.645678371044106	38.0	35.0	40.0	27.0	41.0
37	35.59365497034278	38.0	35.0	40.0	27.0	41.0
38	35.41627396435863	38.0	34.0	40.0	26.0	41.0
39	35.33239582635511	38.0	34.0	40.0	25.0	41.0
40	35.18118740877976	38.0	34.0	40.0	25.0	41.0
41	35.06649783487504	38.0	34.0	40.0	24.0	41.0
42	34.82624016505394	38.0	34.0	40.0	23.0	41.0
43	34.47734846230186	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	4.0
15	17.0
16	37.0
17	87.0
18	198.0
19	420.0
20	1013.0
21	1980.0
22	3773.0
23	6500.0
24	10420.0
25	16265.0
26	23355.0
27	32975.0
28	44210.0
29	58039.0
30	74121.0
31	91467.0
32	112348.0
33	137935.0
34	169685.0
35	208372.0
36	268509.0
37	367291.0
38	506870.0
39	595815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98047635311616	21.574594402852714	13.919288007195485	23.52564123683564
2	18.7261253286857	23.30526669529339	35.23097045699896	22.73763751902195
3	19.67139300504848	24.69106381234046	32.165201834892606	23.472341347718444
4	13.969988794576881	16.64904036404996	36.204352360588395	33.17661848078477
5	12.96007303841948	38.41702990028601	34.89030509427601	13.732591967018498
6	32.392495112041416	37.38573561717501	14.125019722057186	16.096749548726386
7	26.127964394502644	32.80055408456159	22.00308011859225	19.06840140234351
8	26.39336562808758	35.0558788569663	19.427772976150862	19.122982538795256
9	24.680557584197167	14.651282912956082	21.787610923987987	38.88054857885876
10	16.267484907821576	27.848423315333637	33.94038832044215	21.94370345640263
11	33.04794464132945	22.837904274717	23.319287201840016	20.794863882113532
12	20.606410702734877	27.257987936507238	31.27898135711914	20.856620003638742
13	30.741296761409416	21.289754816849353	26.342701447772882	21.62624697396835
14	20.369123696376594	22.94549244375233	27.91248568206104	28.772898177810042
15	22.843175692321996	29.608893760069233	25.393882885940066	22.154047661668706
16	21.818983180151065	28.535281707851446	26.709650684387416	22.936084427610073
17	21.507127578922088	27.28185574399653	27.460864300166232	23.750152376915146
18	22.48951482092006	26.287461384120515	29.426078068962592	21.79694572599684
19	22.555737004582838	25.472258615747702	30.514296340794218	21.45770803887524
20	23.28513280860764	25.3065750318573	30.142954256175518	21.26533790335954
21	22.16568870887974	26.146304534920027	30.535857903081325	21.152148853118906
22	21.32896098528652	26.3966602640907	30.18673630795036	22.08764244267242
23	22.042615750629732	26.504504682592938	30.673829937478793	20.779049629298537
24	22.097929028415503	26.492387743514783	28.987964694680592	22.421718533389125
25	22.04016307716074	26.64749188512849	29.799506756383266	21.512838281327504
26	21.681450430554015	27.209044288327505	29.82963437227849	21.279870908839985
27	20.986575090428605	27.162480099483368	29.643377616901923	22.207567193186108
28	21.343603811967068	26.77792286378547	29.85335575150098	22.025117572746478
29	21.607540762883946	26.703098019447868	29.424503965094427	22.26485725257375
30	21.640011231048064	26.90191099870301	30.43701882298763	21.0210589472613
31	22.08559244693714	26.71495870905912	30.200061280229658	20.999387563774086
32	21.623501443965747	26.609530356959166	29.631663355557485	22.135304843517602
33	21.402321546956067	26.452778897343904	30.154705124586663	21.990194431113373
34	20.902305622882054	26.685050735564094	30.6500719511896	21.76257169036425
35	20.696134623219937	27.991483732002592	29.75795773567721	21.554423909100258
36	22.17908689529244	26.35701481085312	29.673651661063953	21.790246632790485
37	21.361175203983727	26.674690935687607	29.898565478877178	22.06556838145149
38	21.234624574397092	25.757281237084566	30.831093896027877	22.17700029249046
39	20.727580093516412	25.653829666586496	31.506238027201245	22.112352212695843
40	20.009495873102335	26.10391355167984	31.259140326967	22.62745024825082
41	20.937118943315056	25.302475040386746	31.2298180665392	22.530587949759
42	19.904360377536	26.886060138821318	30.677344215882123	22.53223526776056
43	20.158047349776496	26.62347764937239	30.183917563814354	23.03455743703676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1066.0
1	978.5
2	891.0
3	1601.0
4	2311.0
5	2311.0
6	3567.5
7	4824.0
8	5308.0
9	5792.0
10	8689.5
11	11587.0
12	11587.0
13	20865.0
14	30143.0
15	44844.0
16	59545.0
17	55529.0
18	51513.0
19	51513.0
20	55733.5
21	59954.0
22	50029.5
23	40105.0
24	45330.5
25	50556.0
26	50556.0
27	57441.5
28	64327.0
29	69901.0
30	75475.0
31	83454.5
32	91434.0
33	91434.0
34	101379.5
35	111325.0
36	120732.0
37	130139.0
38	138348.5
39	146558.0
40	146558.0
41	150604.5
42	154651.0
43	161686.0
44	168721.0
45	176739.5
46	184758.0
47	184758.0
48	221461.0
49	258164.0
50	242059.0
51	225954.0
52	207610.5
53	189267.0
54	189267.0
55	173159.5
56	157052.0
57	137257.5
58	117463.0
59	106204.0
60	94945.0
61	94945.0
62	84205.5
63	73466.0
64	63946.0
65	54426.0
66	46829.5
67	39233.0
68	39233.0
69	33848.0
70	28463.0
71	23830.0
72	19197.0
73	15819.5
74	12442.0
75	12442.0
76	9864.5
77	7287.0
78	5669.5
79	4052.0
80	3199.0
81	2346.0
82	2346.0
83	1733.5
84	1121.0
85	849.0
86	577.0
87	449.0
88	321.0
89	321.0
90	237.5
91	154.0
92	108.0
93	62.0
94	43.0
95	24.0
96	24.0
97	19.0
98	14.0
99	11.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2731713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.52214377030939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.125127371153	42.88468586773203
2	11.461918570521927	12.269329072301074
3	3.5193252701985194	5.6508549925814435
4	1.5567167867336413	3.3327527867684807
5	0.8261968025775027	2.2109912025061518
6	0.47823951177693536	1.5357842343580625
7	0.33807678951327597	1.2666216173613931
8	0.23155031178420182	0.9914455261899213
9	0.19385032978201053	0.9337756698463191
>10	1.1037775335229374	11.163539986208049
>50	0.09823681442531254	3.606145474938248
>100	0.059095505871905415	5.989709157933335
>500	0.004527256879635759	1.6975178512776725
>1k	0.0029495764518726775	2.99401514564435
>5k	1.3718960241268266E-4	0.39907474627109524
>10k+	2.743792048253653E-4	3.0737566680823276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27600	1.0103550409578166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25286	0.9256462886108461	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19830	0.7259181326881704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11007	0.4029339831819814	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5540	0.2028031495255907	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5330	0.19511566551830298	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4885	0.17882552083619327	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4797	0.1756040989664727	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4465	0.1634505528216178	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4238	0.15514074868040675	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3099	0.11344529970754615	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2903	0.1062703146340776	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2762	0.10110871822918441	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.321413340274033E-5	0.0
3	0.0	0.0	0.0	7.321413340274033E-5	0.0
4	0.0	0.0	0.0	7.321413340274033E-5	0.0
5	0.0	0.0	0.0	7.321413340274033E-5	0.0
6	0.0	0.0	0.0	7.321413340274033E-5	3.6607066701370166E-5
7	0.0	0.0	0.0	1.0982120010411049E-4	7.321413340274033E-5
8	0.0	0.0	0.0	1.4642826680548066E-4	7.321413340274033E-5
9	0.0	0.0	0.0	3.660706670137017E-4	7.321413340274033E-5
10	3.6607066701370166E-5	0.0	0.0	5.124989338191824E-4	7.321413340274033E-5
11	3.6607066701370166E-5	0.0	0.0	9.517837342356243E-4	7.321413340274033E-5
12	3.6607066701370166E-5	0.0	0.0	0.0012812473345479559	7.321413340274033E-5
13	3.6607066701370166E-5	0.0	0.0	0.0014276756013534364	7.321413340274033E-5
14	7.321413340274033E-5	0.0	0.0	0.0016107109348602873	7.321413340274033E-5
15	7.321413340274033E-5	0.0	0.0	0.001976781601873989	7.321413340274033E-5
16	7.321413340274033E-5	0.0	0.0	0.0027089229359013923	7.321413340274033E-5
17	1.0982120010411049E-4	0.0	0.0	0.003990170270449348	7.321413340274033E-5
18	1.0982120010411049E-4	0.0	0.0	0.0045392762709699	7.321413340274033E-5
19	1.0982120010411049E-4	0.0	0.0	0.0058205236055178565	7.321413340274033E-5
20	1.0982120010411049E-4	0.0	0.0	0.010469621076591867	7.321413340274033E-5
21	1.0982120010411049E-4	0.0	0.0	0.016766036549227537	2.1964240020822098E-4
22	1.0982120010411049E-4	0.0	0.0	0.027565121226131736	2.562494669095912E-4
23	1.0982120010411049E-4	0.0	0.0	0.03492314163310714	2.562494669095912E-4
24	1.0982120010411049E-4	0.0	0.0	0.046381153510636	2.562494669095912E-4
25	1.0982120010411049E-4	0.0	0.0	0.05311685378368811	2.562494669095912E-4
26	1.0982120010411049E-4	0.0	0.0	0.06538022112864712	2.562494669095912E-4
27	1.0982120010411049E-4	0.0	0.0	0.1272095567872613	2.562494669095912E-4
28	1.0982120010411049E-4	0.0	0.0	0.2580798202446597	2.562494669095912E-4
29	1.0982120010411049E-4	0.0	0.0	0.4248982232028035	2.562494669095912E-4
30	1.0982120010411049E-4	0.0	0.0	0.677120912775244	2.562494669095912E-4
31	1.0982120010411049E-4	0.0	0.0	1.0225818012360741	2.562494669095912E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8410	0.0	22.459572	1
TAATACT	655	0.0	17.793892	4
GTATTGG	800	0.0	16.41875	1
CGCGATA	80	3.3846998E-4	16.1875	14
GTATTAG	590	0.0	14.737288	1
GTATCAA	12765	0.0	14.724638	2
CTAATAC	650	0.0	14.515384	3
ATTAGAC	270	0.0	14.388888	3
TAGTACT	375	0.0	13.813333	4
CGAACTA	350	0.0	13.742857	24
TAACGGC	310	0.0	13.725807	36
TATACCG	135	6.574426E-6	13.703703	5
TACCCCG	300	0.0	13.566667	5
TTAACGG	315	0.0	13.507937	35
GGACCCT	1180	0.0	13.169492	6
TGGACCC	1195	0.0	13.158997	5
TTGGACC	1295	0.0	13.142857	4
TACTGGT	635	0.0	13.110235	7
GACGTAT	170	3.7365498E-7	13.058823	28
CGTCTAA	85	0.009409066	13.058823	1
>>END_MODULE