FastQCFastQC Report
Wed 25 May 2016
SRR2088696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937179
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325670.8271658464093199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310220.7879245520714198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT235600.5983979900329652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134230.34092938116351834No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG68890.1749729946238157No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59520.15117422906095965No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55310.14048129384008193No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA52670.13377598529302326No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50880.12922958290694936No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG47170.11980659248665096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97300.023.4054471
GTATTGG10350.016.4444451
TATACTG5000.015.915
GTATCAA147300.015.4355062
TTGGACC15150.014.6534664
ATTGGAC12200.014.5573763
GGACCCT14800.014.256
TGGACCC15500.013.84516055
CTAATAC7400.013.753
TAATACT9800.013.5918364
AATACTG11500.013.3521735
TATACCG1951.0280928E-813.2820525
GACCCTC14250.012.9824567
GTATTAG8150.012.4846621
ATACTGG9000.012.3333336
TCTATAC4350.012.3333333
TAGACAG5950.012.126055
TTAACGG3850.012.01298635
TATACAG5600.011.8928575
TGTACTG11700.011.85897355
Produced by FastQC (version 0.11.5)