##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3937179
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56798230408117	31.0	31.0	34.0	30.0	34.0
2	31.77330520151611	31.0	31.0	34.0	30.0	34.0
3	31.858897195174514	31.0	31.0	34.0	30.0	34.0
4	35.57480292361612	37.0	35.0	37.0	33.0	37.0
5	35.43788941269879	37.0	35.0	37.0	33.0	37.0
6	35.482340274597625	37.0	35.0	37.0	33.0	37.0
7	35.35218617187585	37.0	35.0	37.0	33.0	37.0
8	35.370783243535534	37.0	35.0	37.0	33.0	37.0
9	36.97826260883745	39.0	37.0	39.0	33.0	39.0
10	36.78027415060377	39.0	37.0	39.0	32.0	39.0
11	36.877136650378354	39.0	37.0	39.0	33.0	39.0
12	36.736898931951025	39.0	35.0	39.0	32.0	39.0
13	36.77777515322519	39.0	37.0	39.0	32.0	39.0
14	37.79253343574168	40.0	37.0	41.0	32.0	41.0
15	37.801246781007414	40.0	37.0	41.0	32.0	41.0
16	37.69556299065905	39.0	37.0	41.0	32.0	41.0
17	37.69410204616046	39.0	37.0	41.0	32.0	41.0
18	37.644211502702824	39.0	37.0	41.0	32.0	41.0
19	37.68384317807242	40.0	37.0	41.0	32.0	41.0
20	37.555568085677585	39.0	36.0	41.0	32.0	41.0
21	37.49313912321487	39.0	36.0	41.0	32.0	41.0
22	37.35525893031534	39.0	36.0	41.0	31.0	41.0
23	37.18364366974425	39.0	36.0	41.0	31.0	41.0
24	37.03205493070038	39.0	36.0	41.0	31.0	41.0
25	37.226006996379894	39.0	36.0	41.0	31.0	41.0
26	37.12312521223952	39.0	36.0	41.0	31.0	41.0
27	37.00459999405666	39.0	36.0	41.0	31.0	41.0
28	36.852799936197975	39.0	36.0	41.0	30.0	41.0
29	36.748741675194346	39.0	35.0	41.0	30.0	41.0
30	36.58276420757095	39.0	35.0	40.0	30.0	41.0
31	36.46865890527202	39.0	35.0	40.0	30.0	41.0
32	36.29873165532987	38.0	35.0	40.0	30.0	41.0
33	36.1843718560929	38.0	35.0	40.0	30.0	41.0
34	36.01712088782349	38.0	35.0	40.0	29.0	41.0
35	35.90104742507262	38.0	35.0	40.0	28.0	41.0
36	35.716911270734705	38.0	35.0	40.0	27.0	41.0
37	35.68661800746169	38.0	35.0	40.0	27.0	41.0
38	35.51471472340983	38.0	34.0	40.0	26.0	41.0
39	35.448795698646165	38.0	34.0	40.0	26.0	41.0
40	35.3209467997264	38.0	34.0	40.0	25.0	41.0
41	35.21500622653936	38.0	34.0	40.0	25.0	41.0
42	35.002755272239334	38.0	34.0	40.0	24.0	41.0
43	34.662602335326895	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	8.0
15	22.0
16	43.0
17	116.0
18	270.0
19	662.0
20	1449.0
21	2955.0
22	5517.0
23	9324.0
24	15165.0
25	22912.0
26	33601.0
27	47287.0
28	63822.0
29	82718.0
30	105772.0
31	131689.0
32	161159.0
33	198126.0
34	243723.0
35	300424.0
36	384996.0
37	525557.0
38	737712.0
39	862143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40030209446916	21.34604497280921	13.30193521808381	23.951717714637816
2	19.073631145548628	23.121630995187164	34.64213844481036	23.16259941445385
3	19.841744558731012	24.664563130098987	31.69429177591367	23.799400535256336
4	14.137812885825104	16.592793977616967	36.38008330329914	32.88930983325879
5	13.214283627947829	38.04645407282727	34.90867445955594	13.830587839668961
6	32.52689298606947	37.383466690236844	14.252869884757589	15.836770438936101
7	26.520866844001755	32.26068715697203	22.123276589659753	19.095169409366452
8	26.537731710953448	34.751074309804046	19.534849698223017	19.17634428101948
9	24.972524744239465	14.315224174466032	21.49541588025335	39.21683520104115
10	16.200533427614037	27.525545574636052	34.093420695376054	22.180500302373858
11	33.55450691980222	22.69353260291188	23.008885295791735	20.74307518149416
12	20.54938320050981	26.957016686312713	31.136100238267044	21.357499874910438
13	30.734696085699937	21.208687743178555	25.94263557740199	22.11398059371951
14	20.666853094563393	22.59998846890121	27.511195198389505	29.221963238145893
15	23.390275118301705	29.32160310719934	24.846927203462172	22.441194571036778
16	22.27132675451129	28.25144602264718	26.19657373972583	23.280653483115703
17	21.66264221159363	27.14029511993232	27.231019976485705	23.966042691988353
18	22.559375634178686	26.24399347857946	28.93096808654115	22.265662800700706
19	22.877242817763683	25.499374044207794	29.725191564823444	21.89819157320508
20	23.600730370653707	25.416065665289793	29.40003489808312	21.58316906597338
21	22.702523812099983	26.102623223378973	29.52723256930914	21.6676203952119
22	21.854403876481104	26.270052745887345	29.45375356314762	22.421789814483922
23	22.34119911743916	26.445203532783246	29.836692718314307	21.37690463146329
24	22.46588229796004	26.439717371244743	28.38661894722084	22.707781383574382
25	22.447899879583836	26.56302901138099	29.092784453030962	21.89628665600421
26	22.18745959988103	26.91376236640498	29.002821563357926	21.895956470356058
27	21.564831063053013	26.892198703691143	28.963224684475865	22.57974554877998
28	21.82389980237119	26.703307114052983	29.03342723305189	22.43936585052394
29	21.9438333893379	26.61590951287711	28.872423631234444	22.567833466550542
30	22.16249248510164	26.836041744609528	29.508513582948602	21.492952187340226
31	22.512413075453264	26.74107527242221	29.32330483323212	21.42320681889241
32	21.93392781989338	26.628558163090883	28.99647183935503	22.441042177660705
33	21.81638172915176	26.54443701950051	29.38781802910155	22.25136322224618
34	21.36171609164836	26.535014029080212	29.959242391570207	22.14402748770122
35	21.26352395966757	27.66193764621827	29.125803017846025	21.948735376268136
36	22.49796110362267	26.592415534066394	28.952023771334755	21.95759959097618
37	21.815949947919563	26.638565328119444	29.17675320324527	22.36873152071572
38	21.727282401943118	26.00669159314321	29.914845121341955	22.351180883571715
39	21.30053015115645	25.717906145491483	30.562669363013466	22.418894340338603
40	20.692226591678967	25.98182607394787	30.437529002364382	22.88841833200878
41	21.41467278983252	25.333798641108267	30.40351988060487	22.848008688454346
42	20.464474691143074	26.54971998987092	30.113566083736604	22.8722392352494
43	20.74531028434318	26.22385215404227	29.786250510835295	23.24458705077925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1100.0
1	1068.0
2	1036.0
3	1924.0
4	2812.0
5	2812.0
6	4273.0
7	5734.0
8	6272.0
9	6810.0
10	10217.5
11	13625.0
12	13625.0
13	24961.0
14	36297.0
15	54052.5
16	71808.0
17	67933.0
18	64058.0
19	64058.0
20	71319.5
21	78581.0
22	68434.0
23	58287.0
24	66342.0
25	74397.0
26	74397.0
27	83678.5
28	92960.0
29	101769.5
30	110579.0
31	123119.5
32	135660.0
33	135660.0
34	150081.0
35	164502.0
36	178120.5
37	191739.0
38	201216.5
39	210694.0
40	210694.0
41	216050.0
42	221406.0
43	230384.5
44	239363.0
45	246695.5
46	254028.0
47	254028.0
48	296502.5
49	338977.0
50	324571.0
51	310165.0
52	291644.0
53	273123.0
54	273123.0
55	254985.5
56	236848.0
57	211992.5
58	187137.0
59	170993.0
60	154849.0
61	154849.0
62	137538.0
63	120227.0
64	105879.0
65	91531.0
66	78952.5
67	66374.0
68	66374.0
69	56594.5
70	46815.0
71	39026.5
72	31238.0
73	25466.0
74	19694.0
75	19694.0
76	15614.5
77	11535.0
78	8937.5
79	6340.0
80	4885.5
81	3431.0
82	3431.0
83	2594.0
84	1757.0
85	1283.0
86	809.0
87	637.0
88	465.0
89	465.0
90	358.5
91	252.0
92	174.5
93	97.0
94	62.5
95	28.0
96	28.0
97	18.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3937179.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.85105107073143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43623242248763	43.023097904016296
2	12.468530448436383	13.678240008083076
3	4.104265600854923	6.753698462410189
4	1.8081935935985967	3.9672527659298407
5	0.9126739049361267	2.5030561485287683
6	0.5282579984886732	1.738530387217475
7	0.32477187272664565	1.246985500408643
8	0.2161991443218127	0.9487000245315347
9	0.1633134693954608	0.8062123905313878
>10	0.890457956049992	9.182622924947994
>50	0.08526015844152653	3.2267717241190637
>100	0.05475260349138525	5.702457772020025
>500	0.004217419872597789	1.5600787584182831
>1k	0.0024562994453078825	2.375402272890873
>5k	2.3172636276489435E-4	0.7302653071213046
>10k+	1.8538109021191547E-4	2.556627648825281
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32567	0.8271658464093199	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31022	0.7879245520714198	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23560	0.5983979900329652	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13423	0.34092938116351834	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6889	0.1749729946238157	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5952	0.15117422906095965	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5531	0.14048129384008193	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5267	0.13377598529302326	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5088	0.12922958290694936	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4717	0.11980659248665096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.539889601158596E-5	0.0	0.0	0.0	2.539889601158596E-5
2	2.539889601158596E-5	0.0	0.0	0.0	2.539889601158596E-5
3	2.539889601158596E-5	0.0	0.0	0.0	2.539889601158596E-5
4	2.539889601158596E-5	0.0	0.0	0.0	2.539889601158596E-5
5	2.539889601158596E-5	0.0	0.0	0.0	2.539889601158596E-5
6	2.539889601158596E-5	0.0	0.0	0.0	2.539889601158596E-5
7	2.539889601158596E-5	0.0	2.539889601158596E-5	2.539889601158596E-5	2.539889601158596E-5
8	2.539889601158596E-5	0.0	2.539889601158596E-5	5.079779202317192E-5	2.539889601158596E-5
9	2.539889601158596E-5	0.0	2.539889601158596E-5	1.5239337606951577E-4	2.539889601158596E-5
10	2.539889601158596E-5	0.0	2.539889601158596E-5	2.2859006410427365E-4	2.539889601158596E-5
11	2.539889601158596E-5	0.0	2.539889601158596E-5	2.539889601158596E-4	2.539889601158596E-5
12	2.539889601158596E-5	0.0	2.539889601158596E-5	4.317812321969613E-4	2.539889601158596E-5
13	2.539889601158596E-5	0.0	2.539889601158596E-5	4.571801282085473E-4	2.539889601158596E-5
14	2.539889601158596E-5	0.0	2.539889601158596E-5	5.841746082664771E-4	2.539889601158596E-5
15	2.539889601158596E-5	0.0	2.539889601158596E-5	7.365679843359929E-4	2.539889601158596E-5
16	2.539889601158596E-5	0.0	2.539889601158596E-5	0.0012191470085561261	2.539889601158596E-5
17	2.539889601158596E-5	0.0	2.539889601158596E-5	0.0018287205128341892	2.539889601158596E-5
18	5.079779202317192E-5	0.0	2.539889601158596E-5	0.0021589061609848066	2.539889601158596E-5
19	5.079779202317192E-5	0.0	2.539889601158596E-5	0.002590687393181768	2.539889601158596E-5
20	5.079779202317192E-5	0.0	2.539889601158596E-5	0.004851189138212918	2.539889601158596E-5
21	5.079779202317192E-5	0.0	2.539889601158596E-5	0.008076848931684335	2.539889601158596E-5
22	5.079779202317192E-5	0.0	2.539889601158596E-5	0.01214067229353809	2.539889601158596E-5
23	5.079779202317192E-5	0.0	2.539889601158596E-5	0.015620321047125366	2.539889601158596E-5
24	5.079779202317192E-5	0.0	2.539889601158596E-5	0.02143666823377855	2.539889601158596E-5
25	5.079779202317192E-5	0.0	2.539889601158596E-5	0.02636405406002623	2.539889601158596E-5
26	5.079779202317192E-5	0.0	2.539889601158596E-5	0.035685448896278275	2.539889601158596E-5
27	5.079779202317192E-5	0.0	2.539889601158596E-5	0.09115663778558201	2.539889601158596E-5
28	5.079779202317192E-5	0.0	2.539889601158596E-5	0.19762880986615036	5.079779202317192E-5
29	5.079779202317192E-5	0.0	2.539889601158596E-5	0.3274679662773778	5.079779202317192E-5
30	5.079779202317192E-5	0.0	2.539889601158596E-5	0.5035839112217149	5.079779202317192E-5
31	5.079779202317192E-5	0.0	2.539889601158596E-5	0.7334439201265678	5.079779202317192E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9730	0.0	23.405447	1
GTATTGG	1035	0.0	16.444445	1
TATACTG	500	0.0	15.91	5
GTATCAA	14730	0.0	15.435506	2
TTGGACC	1515	0.0	14.653466	4
ATTGGAC	1220	0.0	14.557376	3
GGACCCT	1480	0.0	14.25	6
TGGACCC	1550	0.0	13.8451605	5
CTAATAC	740	0.0	13.75	3
TAATACT	980	0.0	13.591836	4
AATACTG	1150	0.0	13.352173	5
TATACCG	195	1.0280928E-8	13.282052	5
GACCCTC	1425	0.0	12.982456	7
GTATTAG	815	0.0	12.484662	1
ATACTGG	900	0.0	12.333333	6
TCTATAC	435	0.0	12.333333	3
TAGACAG	595	0.0	12.12605	5
TTAACGG	385	0.0	12.012986	35
TATACAG	560	0.0	11.892857	5
TGTACTG	1170	0.0	11.8589735	5
>>END_MODULE