FastQCFastQC Report
Wed 25 May 2016
SRR2088695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734671
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194651.1221147987139923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177101.0209428761995791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141290.8145060360148985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73540.42394206163589526No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG47430.2734236059748506No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG42110.2427549662154956No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA37970.21888876910953142No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36860.21248986118981641No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG34100.1965790631191736No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34040.1962331762045944No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA22980.1324746882838302No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC21890.12619107600230822No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.12353927632386777No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20080.11575682074583596No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19740.11379679489655387No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.1122403037809475No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.10180604852447525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58100.023.6265071
GTATTGG5900.019.4406781
ATTGGAC5900.019.127123
TACGTTA609.239398E-418.519
CGCGCTA500.007036293418.49999814
TTGGACC7550.017.8874154
ACGTTAA650.001580372117.07692320
CGTTTAT1352.2213499E-816.44444527
TCACGTT1251.6590275E-716.27999924
TAGGTCG803.3838072E-416.187521
TGGACCC8050.015.8571435
CGCTCTA700.002593206715.85714314
ACGTTTA1302.5909867E-715.65384626
GTATCAA88450.015.5195032
GGACCCT8000.015.4937496
TAGGACC2950.015.0508474
ATTACGT750.004105525614.817
GACCCTC7900.014.7531647
TAATACT4400.014.7159094
TATACTG2450.014.3469385
Produced by FastQC (version 0.11.5)