##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1734671
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53683782111997	31.0	31.0	34.0	30.0	34.0
2	31.74255291060956	31.0	31.0	34.0	30.0	34.0
3	31.818029470718077	31.0	31.0	34.0	30.0	34.0
4	35.541176395985175	37.0	35.0	37.0	33.0	37.0
5	35.41980006583381	37.0	35.0	37.0	33.0	37.0
6	35.46638815083667	37.0	35.0	37.0	33.0	37.0
7	35.32634257447089	37.0	35.0	37.0	32.0	37.0
8	35.35830483129077	37.0	35.0	37.0	33.0	37.0
9	36.94660658995279	39.0	37.0	39.0	33.0	39.0
10	36.7598230442545	39.0	37.0	39.0	32.0	39.0
11	36.85046559261093	39.0	37.0	39.0	32.0	39.0
12	36.70082223084378	39.0	35.0	39.0	32.0	39.0
13	36.746391102405006	39.0	35.0	39.0	32.0	39.0
14	37.71771995957735	40.0	37.0	41.0	32.0	41.0
15	37.733170728051604	39.0	37.0	41.0	32.0	41.0
16	37.62006858937516	39.0	36.0	41.0	32.0	41.0
17	37.648012216725824	39.0	36.0	41.0	32.0	41.0
18	37.611930446753306	39.0	36.0	41.0	32.0	41.0
19	37.66337766642781	39.0	37.0	41.0	32.0	41.0
20	37.54297097259365	39.0	36.0	41.0	32.0	41.0
21	37.485146174692495	39.0	36.0	41.0	32.0	41.0
22	37.348468960396524	39.0	36.0	41.0	31.0	41.0
23	37.17223900094024	39.0	36.0	41.0	31.0	41.0
24	37.02097919432561	39.0	36.0	41.0	31.0	41.0
25	37.20259634247647	39.0	36.0	41.0	31.0	41.0
26	37.079979431258145	39.0	36.0	41.0	31.0	41.0
27	36.976494101763386	39.0	36.0	41.0	31.0	41.0
28	36.80299203710675	39.0	35.0	41.0	30.0	41.0
29	36.68891853267853	39.0	35.0	40.0	30.0	41.0
30	36.49840805547565	39.0	35.0	40.0	30.0	41.0
31	36.352346352708956	38.0	35.0	40.0	30.0	41.0
32	36.178682874158845	38.0	35.0	40.0	30.0	41.0
33	36.049510252952864	38.0	35.0	40.0	29.0	41.0
34	35.86166886977416	38.0	35.0	40.0	28.0	41.0
35	35.73210770226746	38.0	35.0	40.0	27.0	41.0
36	35.53984300192947	38.0	34.0	40.0	27.0	41.0
37	35.47393597979098	38.0	34.0	40.0	26.0	41.0
38	35.28532211583637	38.0	34.0	40.0	25.0	41.0
39	35.19669551171375	38.0	34.0	40.0	25.0	41.0
40	35.03242805119818	38.0	34.0	40.0	24.0	41.0
41	34.91228365494091	38.0	34.0	40.0	24.0	41.0
42	34.64849818784081	38.0	33.0	40.0	23.0	41.0
43	34.2943111402681	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	4.0
15	4.0
16	23.0
17	54.0
18	136.0
19	284.0
20	623.0
21	1279.0
22	2407.0
23	4286.0
24	6919.0
25	10564.0
26	15536.0
27	21549.0
28	29265.0
29	37998.0
30	48276.0
31	59712.0
32	73290.0
33	89657.0
34	109724.0
35	135175.0
36	173213.0
37	237573.0
38	319401.0
39	357715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.11154218869169	21.601444884937834	13.884765468495178	23.402247457875298
2	18.918342440727955	23.553227096089113	34.844763070345905	22.68366739283703
3	19.587864211715075	24.71817422439183	32.700667734688594	22.9932938292045
4	13.705653694562253	16.625458083982494	36.62948190175543	33.039406319699815
5	12.564399819908212	38.61602574782192	35.17076148733679	13.648812944933072
6	32.08798671333066	37.62673152430634	13.94074150083791	16.344540261525093
7	25.893382664493732	33.112273163037834	21.884207437606324	19.110136734862117
8	26.736654962237793	34.60321870833143	19.336404424816003	19.323721904614764
9	24.596479678279053	14.382150851660056	21.9145301904511	39.106839279609794
10	16.040563311429086	27.93734373838036	34.067958708020136	21.95413424217042
11	32.88940669441064	22.756707179632336	23.634279929738838	20.719606196218187
12	20.750851314168507	27.374355137083633	31.320175410783946	20.554618137963914
13	31.019714977652825	21.24696844531326	26.386905643779134	21.346410933254777
14	20.393261892312722	23.094119864804334	28.066244261880207	28.446373981002736
15	22.654324652916895	29.75889952619257	25.56680776931188	22.019968051578655
16	21.67171757641651	28.519010233064368	26.833099763586294	22.97617242693283
17	21.416741272552546	27.257445359955867	27.36392088182716	23.96189248566443
18	22.53401365446243	26.13423525267904	29.62452245987856	21.707228632979973
19	22.45572791612934	25.321516299056135	30.771195229527677	21.451560555286854
20	23.29732842711961	25.06261994349361	30.486587946648097	21.153463682738685
21	22.254133492748768	25.838444292894735	30.970599035782577	20.93682317857392
22	21.1133408006475	26.176318160619505	30.607590718931714	22.102750319801277
23	21.98088283023121	26.337789701908893	31.15190142684117	20.529426041018727
24	21.86501071384718	26.445706419257597	29.212225257700165	22.477057609195057
25	21.81745126309254	26.580256429028903	30.299751364956233	21.302540942922317
26	21.587436464897376	27.319647356761024	30.097176928651027	20.995739249690576
27	20.674756192961084	27.281542148338218	29.88013288975258	22.163568768948117
28	21.120777369310954	26.87541326280315	30.041489135403776	21.962320232482124
29	21.390857401778206	26.80329584111339	29.530844753846697	22.27500200326171
30	21.396968070602437	27.11672703354123	30.769696386231164	20.716608509625168
31	22.141143767319566	26.832407989757133	30.517371882045648	20.509076360877653
32	21.542817052916664	26.71232758257906	29.80870724189198	21.936148122612302
33	21.27838650672087	26.715959395182143	30.316065697760553	21.689588400336433
34	20.678388005564166	27.098510322706726	30.913988877429784	21.309112794299324
35	20.391590105558922	28.775600675863032	29.825079222515395	21.007729996062654
36	22.178268962817732	27.02783409649438	29.5806524695461	21.213244471141792
37	21.32709891385744	27.44405135037134	29.78288101893673	21.44596871683449
38	21.200561950940553	26.31594117847131	30.768889316763815	21.71460755382433
39	20.750736018530315	26.10760196025644	31.455301898746217	21.686360122467025
40	19.886998745006977	26.62966060999463	31.074192166699042	22.409148478299343
41	21.109363101129837	25.607795368689512	31.158876812951853	22.1239647172288
42	19.996414305652195	27.349681870510317	30.52198370757337	22.131920116264123
43	20.33607525576896	27.0473767071681	29.9696023049904	22.64694573207254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	640.0
1	565.0
2	490.0
3	882.0
4	1274.0
5	1274.0
6	2083.5
7	2893.0
8	3102.0
9	3311.0
10	5110.0
11	6909.0
12	6909.0
13	12895.0
14	18881.0
15	29612.0
16	40343.0
17	37617.5
18	34892.0
19	34892.0
20	37535.0
21	40178.0
22	33220.0
23	26262.0
24	29570.0
25	32878.0
26	32878.0
27	36942.0
28	41006.0
29	44581.0
30	48156.0
31	52988.0
32	57820.0
33	57820.0
34	64491.0
35	71162.0
36	77489.5
37	83817.0
38	88181.0
39	92545.0
40	92545.0
41	94511.5
42	96478.0
43	100868.0
44	105258.0
45	110253.5
46	115249.0
47	115249.0
48	143753.5
49	172258.0
50	160607.5
51	148957.0
52	135818.5
53	122680.0
54	122680.0
55	110683.5
56	98687.0
57	85537.0
58	72387.0
59	64881.5
60	57376.0
61	57376.0
62	50392.5
63	43409.0
64	37815.0
65	32221.0
66	27501.0
67	22781.0
68	22781.0
69	19510.0
70	16239.0
71	13566.0
72	10893.0
73	8991.5
74	7090.0
75	7090.0
76	5652.5
77	4215.0
78	3279.5
79	2344.0
80	1805.5
81	1267.0
82	1267.0
83	971.0
84	675.0
85	539.5
86	404.0
87	282.0
88	160.0
89	160.0
90	136.0
91	112.0
92	77.5
93	43.0
94	31.5
95	20.0
96	20.0
97	15.5
98	11.0
99	5.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1734671.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.30645460028262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.55069884799214	46.481371769060715
2	10.478546452921101	11.80019600256709
3	3.0276580256861125	5.114300675054291
4	1.1822204264288345	2.6626656307296765
5	0.6485435465034376	1.8258593878751044
6	0.39087381897634904	1.3205231365578531
7	0.26264056300053545	1.0351851255747622
8	0.19043261849463042	0.8578068470144677
9	0.14691172985799872	0.7444870782748549
>10	0.9701729831128248	10.474188159997638
>50	0.08943029118662547	3.51496260705831
>100	0.053970480512459146	5.6562541744637755
>500	0.00471960915639992	1.8957978601228238
>1k	0.0027702053744086486	3.2276975329998256
>5k	1.0260019905217217E-4	0.4248445107064617
>10k+	3.078005971565165E-4	2.9638595019423866
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19465	1.1221147987139923	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17710	1.0209428761995791	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14129	0.8145060360148985	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7354	0.42394206163589526	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4743	0.2734236059748506	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4211	0.2427549662154956	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3797	0.21888876910953142	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3686	0.21248986118981641	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3410	0.1965790631191736	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3404	0.1962331762045944	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2298	0.1324746882838302	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2189	0.12619107600230822	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2143	0.12353927632386777	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2008	0.11575682074583596	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1974	0.11379679489655387	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1947	0.1122403037809475	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1766	0.10180604852447525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.152956381930637E-4	0.0
5	5.764781909653185E-5	0.0	0.0	1.152956381930637E-4	0.0
6	5.764781909653185E-5	0.0	0.0	1.152956381930637E-4	0.0
7	5.764781909653185E-5	0.0	0.0	1.152956381930637E-4	0.0
8	5.764781909653185E-5	0.0	0.0	1.152956381930637E-4	0.0
9	5.764781909653185E-5	0.0	0.0	2.305912763861274E-4	0.0
10	5.764781909653185E-5	0.0	0.0	2.8823909548265924E-4	0.0
11	5.764781909653185E-5	0.0	0.0	4.611825527722548E-4	0.0
12	5.764781909653185E-5	0.0	0.0	6.917738291583822E-4	0.0
13	5.764781909653185E-5	0.0	0.0	6.917738291583822E-4	0.0
14	5.764781909653185E-5	0.0	0.0	8.647172864479777E-4	0.0
15	5.764781909653185E-5	0.0	0.0	0.001152956381930637	0.0
16	5.764781909653185E-5	0.0	0.0	0.0016141389347028917	0.0
17	5.764781909653185E-5	0.0	0.0	0.002305912763861274	0.0
18	5.764781909653185E-5	0.0	0.0	0.0029976865930196562	0.0
19	5.764781909653185E-5	0.0	0.0	0.0036318126030815065	0.0
20	5.764781909653185E-5	0.0	0.0	0.0058800775478462484	0.0
21	5.764781909653185E-5	0.0	0.0	0.010491903075568796	0.0
22	5.764781909653185E-5	0.0	0.0	0.016371980623415047	0.0
23	5.764781909653185E-5	0.0	0.0	0.019715554131013894	0.0
24	5.764781909653185E-5	0.0	0.0	0.02640270114621159	0.0
25	5.764781909653185E-5	0.0	0.0	0.031129822312127197	0.0
26	5.764781909653185E-5	0.0	0.0	0.039488756081124315	0.0
27	5.764781909653185E-5	0.0	0.0	0.08704820683576309	0.0
28	5.764781909653185E-5	0.0	0.0	0.1894883813703002	0.0
29	5.764781909653185E-5	5.764781909653185E-5	0.0	0.3169477093927321	0.0
30	5.764781909653185E-5	5.764781909653185E-5	0.0	0.489602927586845	0.0
31	5.764781909653185E-5	5.764781909653185E-5	0.0	0.6952903461232707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5810	0.0	23.626507	1
GTATTGG	590	0.0	19.440678	1
ATTGGAC	590	0.0	19.12712	3
TACGTTA	60	9.239398E-4	18.5	19
CGCGCTA	50	0.0070362934	18.499998	14
TTGGACC	755	0.0	17.887415	4
ACGTTAA	65	0.0015803721	17.076923	20
CGTTTAT	135	2.2213499E-8	16.444445	27
TCACGTT	125	1.6590275E-7	16.279999	24
TAGGTCG	80	3.3838072E-4	16.1875	21
TGGACCC	805	0.0	15.857143	5
CGCTCTA	70	0.0025932067	15.857143	14
ACGTTTA	130	2.5909867E-7	15.653846	26
GTATCAA	8845	0.0	15.519503	2
GGACCCT	800	0.0	15.493749	6
TAGGACC	295	0.0	15.050847	4
ATTACGT	75	0.0041055256	14.8	17
GACCCTC	790	0.0	14.753164	7
TAATACT	440	0.0	14.715909	4
TATACTG	245	0.0	14.346938	5
>>END_MODULE