FastQCFastQC Report
Wed 25 May 2016
SRR2088694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724040
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69570.9608585161040826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68270.942903706977515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51460.7107342135793603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.4021877244351141No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG18560.2563394287608419No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG14980.20689464670460195No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA14550.20095574830119883No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13510.18659190099994477No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12580.173747306778631No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12140.16767029445886966No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC8240.11380586707916689No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8030.11090547483564445No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.1104911330865698No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.10814319650848019No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7600.1049665764322413No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.10123750069056958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.023.5220431
GTCTAAC851.244005E-619.5882361
CTATCCT701.2184065E-418.54
CGCCGAT500.007032429318.537
GTATTGG2700.017.8148161
CTAACCT752.0659348E-417.26666633
ATTTCGG752.0659348E-417.26666629
TTCGGTT650.001579170717.07692331
ACGGTGT650.001579170717.07692329
CTCTAAT1655.4569682E-1116.8181821
GTATTAT904.4423025E-516.4444451
ATATACT1251.6551166E-716.2799994
GGCGTTT700.002591251215.85714226
ACGGGAC700.002591251215.8571423
CTAATAC1902.7284841E-1115.5789483
TTGGACC3000.015.4166674
TTAGCCT1201.9329946E-615.4166674
GTAGACC855.360746E-415.2352944
TCTAATA1701.4824764E-915.2352942
GAGGTGT1101.4503134E-515.1363646
Produced by FastQC (version 0.11.5)