##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724040
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613050383956686	31.0	31.0	34.0	30.0	34.0
2	31.82230954090934	31.0	31.0	34.0	30.0	34.0
3	31.90342798740401	31.0	31.0	34.0	30.0	34.0
4	35.61176868681288	37.0	35.0	37.0	33.0	37.0
5	35.49087343240705	37.0	35.0	37.0	33.0	37.0
6	35.53477570299984	37.0	35.0	37.0	33.0	37.0
7	35.4	37.0	35.0	37.0	33.0	37.0
8	35.42394895309651	37.0	35.0	37.0	33.0	37.0
9	37.033024418540414	39.0	37.0	39.0	33.0	39.0
10	36.84835368211701	39.0	37.0	39.0	32.0	39.0
11	36.93389315507431	39.0	37.0	39.0	33.0	39.0
12	36.804321584442846	39.0	37.0	39.0	32.0	39.0
13	36.83711811502127	39.0	37.0	39.0	32.0	39.0
14	37.843086017347105	40.0	37.0	41.0	32.0	41.0
15	37.85200679520469	40.0	37.0	41.0	32.0	41.0
16	37.73838185735595	40.0	37.0	41.0	32.0	41.0
17	37.75621788851445	39.0	37.0	41.0	32.0	41.0
18	37.714083476051044	39.0	37.0	41.0	32.0	41.0
19	37.75350118777968	40.0	37.0	41.0	32.0	41.0
20	37.624447544334565	39.0	37.0	41.0	32.0	41.0
21	37.57013010330921	39.0	37.0	41.0	32.0	41.0
22	37.439083476051046	39.0	36.0	41.0	32.0	41.0
23	37.26828075796917	39.0	36.0	41.0	31.0	41.0
24	37.118192365062704	39.0	36.0	41.0	31.0	41.0
25	37.3065493619137	39.0	36.0	41.0	31.0	41.0
26	37.195005800784486	39.0	36.0	41.0	31.0	41.0
27	37.08071101044141	39.0	36.0	41.0	31.0	41.0
28	36.920324843931276	39.0	36.0	41.0	30.0	41.0
29	36.812964753328544	39.0	35.0	41.0	30.0	41.0
30	36.62582868349815	39.0	35.0	40.0	30.0	41.0
31	36.4818946467046	39.0	35.0	40.0	30.0	41.0
32	36.31554334014695	38.0	35.0	40.0	30.0	41.0
33	36.18079525993039	38.0	35.0	40.0	30.0	41.0
34	36.00323186564278	38.0	35.0	40.0	29.0	41.0
35	35.870177062040774	38.0	35.0	40.0	28.0	41.0
36	35.6791185569858	38.0	35.0	40.0	27.0	41.0
37	35.61912463399812	38.0	35.0	40.0	27.0	41.0
38	35.437187171979446	38.0	34.0	40.0	26.0	41.0
39	35.35651345229545	38.0	34.0	40.0	25.0	41.0
40	35.21024252803713	38.0	34.0	40.0	25.0	41.0
41	35.08210596099663	38.0	34.0	40.0	24.0	41.0
42	34.82958952544058	38.0	34.0	40.0	23.0	41.0
43	34.48334069940887	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	0.0
15	1.0
16	8.0
17	28.0
18	32.0
19	138.0
20	247.0
21	478.0
22	934.0
23	1633.0
24	2642.0
25	4126.0
26	5904.0
27	8464.0
28	11284.0
29	15204.0
30	19131.0
31	23801.0
32	29611.0
33	36610.0
34	45320.0
35	55422.0
36	71207.0
37	98436.0
38	135730.0
39	157645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48127175294183	21.2470305507983	13.455057731617037	23.81663996464284
2	18.814153914148392	23.195127341030883	34.860643058394565	23.130075686426164
3	19.772388265841666	24.77915584774322	32.26078669686758	23.187669189547538
4	13.694685376498535	16.5649687862549	36.69590077896249	33.04444505828407
5	12.748052593779349	38.468178553671066	35.20454671012651	13.57922214242307
6	32.28882382188829	37.29724877078615	14.17725540025413	16.23667200707143
7	26.108778520523728	32.79998342633004	22.065493619137065	19.02574443400917
8	26.54991436937186	34.63786531130877	19.53055079829844	19.281669521020937
9	24.645599690624827	14.523921330313241	21.84741174520745	38.983067233854484
10	16.177144909121044	27.630931992707588	34.27103474946136	21.920888348710015
11	32.834650019335946	22.77429423788741	23.602977736036685	20.78807800673996
12	20.576763714711895	27.08206728910005	31.573393735152756	20.7677752610353
13	30.842356775868733	21.29854704159991	26.226451577260924	21.632644605270425
14	20.339345892492126	23.028147616153806	27.9372686591901	28.69523783216397
15	22.770841389978454	29.74131263466107	25.360891663444008	22.12695431191647
16	21.799348102314788	28.5199712723054	26.913015855477596	22.767664769902215
17	21.36870891110988	27.29987293519695	27.366443842881612	23.964974310811556
18	22.467128887906746	26.384730125407437	29.303905861554608	21.84423512513121
19	22.457460913761672	25.633804762167834	30.411993812496547	21.496740511573947
20	23.30119882879399	25.359648638196784	30.174161648527704	21.164990884481522
21	22.15236727252638	26.14966023976576	30.576625600795538	21.121346886912328
22	21.24095353847854	26.368294569360806	30.268493453400364	22.12225843876029
23	21.862051820341417	26.50488923263908	30.81942986575327	20.813629081266228
24	21.92075023479366	26.628224959946966	28.970636981382242	22.480387823877134
25	21.966604055024586	26.946853764985363	29.729849179603335	21.35669300038672
26	21.704740069609414	27.224600850781727	29.76824484835092	21.30241423125794
27	20.874813546212916	27.356775868736534	29.543809734268823	22.224600850781727
28	21.25393624661621	26.949616043312524	29.744351140820953	22.052096569250317
29	21.50116015689741	26.839401138058673	29.362051820341417	22.2973868847025
30	21.52892105408541	27.17612286613999	30.366554334014694	20.928401745759903
31	22.1384177669742	26.932628031600466	30.066156565935582	20.862797635489752
32	21.698110601624222	26.80846362079443	29.490359648638197	22.003066128943154
33	21.188470250262416	26.988978509474613	29.919893928512238	21.90265731175073
34	20.85837799016629	27.157201259598917	30.56654328490139	21.417877465333408
35	20.636428926578642	28.694547262582176	29.49892271145241	21.170101099386773
36	22.22957295177062	27.01411524225181	29.309568532125297	21.446743273852274
37	21.429340920391137	27.393652284404173	29.527097950389482	21.649908844815204
38	21.329898900613227	26.41925860449699	30.406469255842218	21.844373239047567
39	20.850367383017513	26.258079664106955	31.084056129495607	21.807496823379925
40	20.198055356057676	26.530854648914428	30.69678470802718	22.57430528700072
41	21.20697751505442	25.871498812220317	30.70092812551793	22.220595547207335
42	20.17374730677863	27.318932655654383	30.199160267388542	22.30815977017844
43	20.570962930224848	26.957074194795865	29.624192033589303	22.847770841389977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	211.0
1	214.0
2	217.0
3	402.0
4	587.0
5	587.0
6	865.5
7	1144.0
8	1253.0
9	1362.0
10	2035.0
11	2708.0
12	2708.0
13	5154.5
14	7601.0
15	11433.5
16	15266.0
17	14245.5
18	13225.0
19	13225.0
20	14641.0
21	16057.0
22	13530.5
23	11004.0
24	12373.5
25	13743.0
26	13743.0
27	15498.0
28	17253.0
29	18912.5
30	20572.0
31	22834.0
32	25096.0
33	25096.0
34	27786.0
35	30476.0
36	32954.5
37	35433.0
38	37103.0
39	38773.0
40	38773.0
41	39582.5
42	40392.0
43	42322.5
44	44253.0
45	46302.5
46	48352.0
47	48352.0
48	59214.5
49	70077.0
50	65769.5
51	61462.0
52	56142.5
53	50823.0
54	50823.0
55	46155.0
56	41487.0
57	35940.0
58	30393.0
59	27368.5
60	24344.0
61	24344.0
62	21556.0
63	18768.0
64	16271.0
65	13774.0
66	11825.0
67	9876.0
68	9876.0
69	8480.5
70	7085.0
71	5980.0
72	4875.0
73	3952.0
74	3029.0
75	3029.0
76	2495.0
77	1961.0
78	1512.5
79	1064.0
80	843.0
81	622.0
82	622.0
83	495.0
84	368.0
85	262.0
86	156.0
87	117.0
88	78.0
89	78.0
90	60.5
91	43.0
92	29.0
93	15.0
94	10.5
95	6.0
96	6.0
97	7.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	724040.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.43324069389153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29312979294639	57.11872372664975
2	7.6911998499612455	10.065202620146733
3	2.009771368221724	3.9451756102963134
4	0.8497813260884832	2.224157841884882
5	0.4911848121870008	1.6069907020507965
6	0.3151731278127821	1.2373679479452246
7	0.21048221170606637	0.9640773254241973
8	0.15524375155854778	0.8126481409562524
9	0.12969029633792734	0.7637450738347545
>10	0.7587669490243418	9.097396240369413
>50	0.058066640617841045	2.6324582413295143
>100	0.03263570918051317	4.053353085078796
>500	0.0027549624627027595	1.2529882136240618
>1k	0.0014834413260707167	1.6007631626910328
>5k	6.357605683160214E-4	2.6249520677184033
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6957	0.9608585161040826	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6827	0.942903706977515	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5146	0.7107342135793603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	0.4021877244351141	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1856	0.2563394287608419	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1498	0.20689464670460195	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1455	0.20095574830119883	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1351	0.18659190099994477	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1258	0.173747306778631	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1214	0.16767029445886966	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	824	0.11380586707916689	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	803	0.11090547483564445	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.1104911330865698	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	783	0.10814319650848019	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	760	0.1049665764322413	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	733	0.10123750069056958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	1.3811391635821225E-4	0.0	0.0
8	0.0	0.0	1.3811391635821225E-4	0.0	0.0
9	0.0	0.0	1.3811391635821225E-4	0.0	0.0
10	0.0	0.0	1.3811391635821225E-4	0.0	0.0
11	0.0	0.0	1.3811391635821225E-4	1.3811391635821225E-4	0.0
12	0.0	0.0	1.3811391635821225E-4	1.3811391635821225E-4	0.0
13	0.0	0.0	1.3811391635821225E-4	2.762278327164245E-4	0.0
14	0.0	0.0	1.3811391635821225E-4	2.762278327164245E-4	0.0
15	0.0	0.0	1.3811391635821225E-4	4.143417490746368E-4	0.0
16	0.0	0.0	1.3811391635821225E-4	0.001104911330865698	0.0
17	0.0	0.0	1.3811391635821225E-4	0.002624164410806033	0.0
18	0.0	0.0	1.3811391635821225E-4	0.0031766200762388818	0.0
19	0.0	0.0	1.3811391635821225E-4	0.004557759239821004	0.0
20	0.0	0.0	1.3811391635821225E-4	0.00552455665432849	0.0
21	0.0	0.0	1.3811391635821225E-4	0.009667974145074858	0.0
22	0.0	0.0	1.3811391635821225E-4	0.015054416883045136	0.0
23	0.0	0.0	1.3811391635821225E-4	0.019335948290149715	0.0
24	0.0	0.0	1.3811391635821225E-4	0.026517871940776754	0.0
25	0.0	0.0	1.3811391635821225E-4	0.03079940334788133	0.0
26	0.0	0.0	1.3811391635821225E-4	0.040053035743881554	0.0
27	0.0	0.0	1.3811391635821225E-4	0.09060272913098724	0.0
28	0.0	0.0	1.3811391635821225E-4	0.1836915087564223	0.0
29	0.0	0.0	1.3811391635821225E-4	0.3063366664825148	0.0
30	0.0	0.0	1.3811391635821225E-4	0.46972542953427987	0.0
31	0.0	0.0	1.3811391635821225E-4	0.65590298878515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2155	0.0	23.522043	1
GTCTAAC	85	1.244005E-6	19.588236	1
CTATCCT	70	1.2184065E-4	18.5	4
CGCCGAT	50	0.0070324293	18.5	37
GTATTGG	270	0.0	17.814816	1
CTAACCT	75	2.0659348E-4	17.266666	33
ATTTCGG	75	2.0659348E-4	17.266666	29
TTCGGTT	65	0.0015791707	17.076923	31
ACGGTGT	65	0.0015791707	17.076923	29
CTCTAAT	165	5.4569682E-11	16.818182	1
GTATTAT	90	4.4423025E-5	16.444445	1
ATATACT	125	1.6551166E-7	16.279999	4
GGCGTTT	70	0.0025912512	15.857142	26
ACGGGAC	70	0.0025912512	15.857142	3
CTAATAC	190	2.7284841E-11	15.578948	3
TTGGACC	300	0.0	15.416667	4
TTAGCCT	120	1.9329946E-6	15.416667	4
GTAGACC	85	5.360746E-4	15.235294	4
TCTAATA	170	1.4824764E-9	15.235294	2
GAGGTGT	110	1.4503134E-5	15.136364	6
>>END_MODULE