FastQCFastQC Report
Wed 25 May 2016
SRR2088693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550538
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT252440.989751966055789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236640.9278042514951748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182260.7145943326466808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105650.41422633185625934No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG63000.2470067099568797No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG55100.21603285267657257No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA51990.20383934683584404No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA50200.19682121967992636No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG43110.16902316295620767No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42300.16584736239961922No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28870.11319180502309709No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28530.11185875293761552No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28280.11087856758064377No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA28120.11025124895218186No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27950.10958472290944106No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27780.10891819686670028No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG27060.10609526303862166No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.10515428509592878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72250.023.7107261
CGCGAAT701.2199373E-418.535
GTATTGG8550.017.3099421
CAATGCG1301.3953468E-817.07692319
ACGTTTA1201.0426447E-716.95833426
TGCGCTA6350.016.60629810
GCGCTAT6450.016.34883711
CGCTATC6850.015.93430612
ATTGGAC9000.015.8277783
GTATCAA112550.015.2372272
TAATACT7450.015.1476514
TTGGACC10650.015.1126764
TTGTGCG7000.014.7999997
GCTATCG7050.014.69503613
GACGCGA908.278891E-414.38888833
TGCGACG1551.2118653E-714.32258122
GGACCCT11500.014.317396
GTGCGCT7250.014.2896569
CTAATAC6250.014.2080013
TATCGGT8000.014.10624915
Produced by FastQC (version 0.11.5)