##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2550538
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569438683132734	31.0	31.0	34.0	30.0	34.0
2	31.773839872215195	31.0	31.0	34.0	30.0	34.0
3	31.854158220736174	31.0	31.0	34.0	30.0	34.0
4	35.57395028029381	37.0	35.0	37.0	33.0	37.0
5	35.44484065714763	37.0	35.0	37.0	33.0	37.0
6	35.484324483697165	37.0	35.0	37.0	33.0	37.0
7	35.35309609188336	37.0	35.0	37.0	33.0	37.0
8	35.378048082404575	37.0	35.0	37.0	33.0	37.0
9	36.97811010853396	39.0	37.0	39.0	33.0	39.0
10	36.78553583596872	39.0	37.0	39.0	32.0	39.0
11	36.88040562422516	39.0	37.0	39.0	33.0	39.0
12	36.73590120986239	39.0	35.0	39.0	32.0	39.0
13	36.77179128481912	39.0	37.0	39.0	32.0	39.0
14	37.76664452754674	40.0	37.0	41.0	32.0	41.0
15	37.781533151045	40.0	37.0	41.0	32.0	41.0
16	37.6649620589852	39.0	36.0	41.0	32.0	41.0
17	37.679289232310985	39.0	37.0	41.0	32.0	41.0
18	37.62620160922911	39.0	36.0	41.0	32.0	41.0
19	37.673202673318336	39.0	37.0	41.0	32.0	41.0
20	37.54955229053635	39.0	36.0	41.0	32.0	41.0
21	37.48170621257162	39.0	36.0	41.0	32.0	41.0
22	37.344794706058096	39.0	36.0	41.0	31.0	41.0
23	37.164772687174235	39.0	36.0	41.0	31.0	41.0
24	37.00850448023123	39.0	36.0	41.0	31.0	41.0
25	37.20055807833484	39.0	36.0	41.0	31.0	41.0
26	37.07768557065215	39.0	36.0	41.0	31.0	41.0
27	36.966711336980666	39.0	36.0	41.0	31.0	41.0
28	36.803673577888276	39.0	35.0	41.0	30.0	41.0
29	36.694027299338416	39.0	35.0	41.0	30.0	41.0
30	36.50270923232667	39.0	35.0	40.0	30.0	41.0
31	36.36381931968863	39.0	35.0	40.0	30.0	41.0
32	36.18997678136926	38.0	35.0	40.0	30.0	41.0
33	36.05708991593146	38.0	35.0	40.0	29.0	41.0
34	35.87798339017102	38.0	35.0	40.0	28.0	41.0
35	35.74507378443293	38.0	35.0	40.0	27.0	41.0
36	35.551628323122415	38.0	34.0	40.0	27.0	41.0
37	35.49832113852058	38.0	34.0	40.0	26.0	41.0
38	35.316726902324135	38.0	34.0	40.0	25.0	41.0
39	35.233629532279075	38.0	34.0	40.0	25.0	41.0
40	35.0783677796606	38.0	34.0	40.0	24.0	41.0
41	34.95407910017416	38.0	34.0	40.0	24.0	41.0
42	34.71241047967135	38.0	33.0	40.0	23.0	41.0
43	34.36480617030603	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	6.0
15	19.0
16	35.0
17	81.0
18	189.0
19	410.0
20	975.0
21	1950.0
22	3745.0
23	6226.0
24	10065.0
25	15699.0
26	22425.0
27	31622.0
28	42631.0
29	55497.0
30	70443.0
31	86682.0
32	107264.0
33	131387.0
34	159661.0
35	197246.0
36	252406.0
37	344748.0
38	472191.0
39	536927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.33037813982776	21.2402638188492	13.45919958847898	23.970158452844068
2	18.768040311495067	23.110692724436962	35.0375097332406	23.083757230827377
3	19.644365228042084	24.77626289041763	32.13823906956101	23.441132811979276
4	13.818574747759099	16.53733447609877	36.386676065990784	33.25741471015135
5	12.839016709415818	38.491016405166285	34.943764805699814	13.726202079718083
6	32.406927479614104	37.14502587297268	14.111297302765141	16.336749344648073
7	26.231171619477927	32.70090467187707	21.97434423639248	19.09357947225252
8	26.71205055560827	34.80865605609483	19.29890870083096	19.18038468746594
9	24.786888099687204	14.420094897625521	21.668447990188735	39.12456901249854
10	16.26386276150365	27.576966114600136	34.118644772201	22.04052635169521
11	33.36899901118901	22.49497949060159	23.32966613318445	20.80635536502495
12	20.809060676610187	26.97701426130487	31.130059618794153	21.08386544329079
13	31.12425692148088	21.076690486477755	26.18439717424324	21.614655417798126
14	20.535863413915028	22.685017827611272	27.73156094910172	29.04755780937198
15	22.96186137983437	29.301582646484782	25.43831928793063	22.298236685750222
16	21.890950066221322	28.33304189155386	26.620344413609992	23.155663628614825
17	21.627789901581547	27.012144104498738	27.264443815383267	24.09562217853645
18	22.677176344755498	26.141661092679268	29.122757629958855	22.058404932606376
19	22.912499245257276	25.32328473443642	30.150776032350823	21.61343998795548
20	23.556441817373432	25.0540866279977	29.87699065844147	21.51248089618739
21	22.395902354718885	25.760604233302935	30.602837518986192	21.240655892991988
22	21.56976292844882	25.95126988894108	30.012609104432087	22.46635807817802
23	22.260009456828325	26.238268161462404	30.723635562379386	20.778086819329882
24	22.2483648547875	26.326053562032797	28.694965532762108	22.7306160504176
25	22.24287581678846	26.430658943328822	29.67526851197669	21.651196727906033
26	22.025431497197843	26.887464527091932	29.580033702693314	21.50707027301691
27	21.251124272604446	26.94776553025283	29.38736846892695	22.413741728215772
28	21.496366648918777	26.58548902231608	29.45739291082901	22.460751417936137
29	21.685464007985765	26.686212869598492	28.999293482394695	22.62902964002105
30	21.72765118574983	26.868174479266727	30.182808489816658	21.221365845166783
31	22.435148976412037	26.59536929071435	29.97979249868067	20.989689234192944
32	21.89036195500714	26.38760920245062	29.571133619652013	22.15089522289023
33	21.550943369594965	26.55067283843644	29.952660967999694	21.945722823968904
34	21.119661812527397	26.589488178572523	30.594329510087675	21.696520498812408
35	20.82254802712212	28.289952943261383	29.498835147721774	21.388663881894722
36	22.500429321186356	26.61720782046768	29.344436350291588	21.53792650805438
37	21.601795385914656	27.024768891896535	29.454413147343818	21.919022574844995
38	21.573056351248248	25.896849997922004	30.40582026223487	22.124273388594876
39	21.175728414946178	25.59220838897519	30.972916302364446	22.25914689371419
40	20.30010923185618	26.019765241686265	30.932571873071485	22.74755365338607
41	21.362786988470667	25.31332605120959	30.778800394269755	22.545086566049985
42	20.19711135454559	26.82779084255949	30.375748175482975	22.59934962741194
43	20.453292599443724	26.680253342628102	29.80422169753989	23.06223236038828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2122.0
1	1417.0
2	712.0
3	1485.5
4	2259.0
5	2259.0
6	3218.0
7	4177.0
8	4448.5
9	4720.0
10	7305.0
11	9890.0
12	9890.0
13	18574.5
14	27259.0
15	40416.5
16	53574.0
17	49923.0
18	46272.0
19	46272.0
20	50266.5
21	54261.0
22	44821.5
23	35382.0
24	40214.5
25	45047.0
26	45047.0
27	50119.0
28	55191.0
29	60604.0
30	66017.0
31	74176.5
32	82336.0
33	82336.0
34	91224.5
35	100113.0
36	108169.0
37	116225.0
38	123360.5
39	130496.0
40	130496.0
41	135181.0
42	139866.0
43	148876.0
44	157886.0
45	166028.5
46	174171.0
47	174171.0
48	214049.5
49	253928.0
50	239098.5
51	224269.0
52	205105.0
53	185941.0
54	185941.0
55	169581.0
56	153221.0
57	133599.0
58	113977.0
59	101372.0
60	88767.0
61	88767.0
62	78393.5
63	68020.0
64	59150.5
65	50281.0
66	42981.0
67	35681.0
68	35681.0
69	30710.0
70	25739.0
71	21386.0
72	17033.0
73	14042.5
74	11052.0
75	11052.0
76	8786.5
77	6521.0
78	5145.5
79	3770.0
80	2904.0
81	2038.0
82	2038.0
83	1574.0
84	1110.0
85	852.5
86	595.0
87	448.0
88	301.0
89	301.0
90	252.0
91	203.0
92	134.0
93	65.0
94	50.5
95	36.0
96	36.0
97	24.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2550538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.19456304753915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18718198270662	42.65522107583581
2	11.362902849211313	12.088893040308665
3	3.602946830051421	5.749715469243048
4	1.5407829938652131	3.2784511243895675
5	0.8117414110252144	2.1590114833539573
6	0.5016942052366666	1.601244241862814
7	0.32633325574692346	1.2151408463136903
8	0.2417358574972284	1.0287226649989802
9	0.1771709707007928	0.8482079134023329
>10	1.0734737909580137	10.752760265417363
>50	0.102983714516036	3.759454239481514
>100	0.06277913999709493	6.2453003402296305
>500	0.004284231359611257	1.5686442374447285
>1k	0.003397838664518057	3.130648004067307
>5k	2.954642316972224E-4	0.8655794776479285
>10k+	2.954642316972224E-4	3.0530055760028323
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25244	0.989751966055789	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23664	0.9278042514951748	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18226	0.7145943326466808	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10565	0.41422633185625934	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6300	0.2470067099568797	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5510	0.21603285267657257	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5199	0.20383934683584404	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5020	0.19682121967992636	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4311	0.16902316295620767	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4230	0.16584736239961922	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2887	0.11319180502309709	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2853	0.11185875293761552	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2828	0.11087856758064377	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2812	0.11025124895218186	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2795	0.10958472290944106	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2778	0.10891819686670028	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2706	0.10609526303862166	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2682	0.10515428509592878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.841482855773959E-5	0.0
5	0.0	0.0	0.0	1.1762224283660938E-4	0.0
6	0.0	0.0	0.0	1.1762224283660938E-4	0.0
7	0.0	0.0	0.0	1.1762224283660938E-4	0.0
8	0.0	0.0	0.0	1.1762224283660938E-4	0.0
9	0.0	0.0	0.0	3.1365931423095835E-4	0.0
10	7.841482855773959E-5	0.0	0.0	3.920741427886979E-4	0.0
11	1.9603707139434896E-4	0.0	0.0	5.881112141830468E-4	0.0
12	1.9603707139434896E-4	0.0	0.0	7.44940871298526E-4	0.0
13	1.9603707139434896E-4	0.0	0.0	8.625631141351354E-4	0.0
14	1.9603707139434896E-4	0.0	0.0	0.0010586001855294844	0.0
15	1.9603707139434896E-4	0.0	0.0	0.001489881742597052	0.0
16	1.9603707139434896E-4	0.0	0.0	0.002274030028174448	0.0
17	1.9603707139434896E-4	0.0	0.0	0.002979763485194104	0.0
18	1.9603707139434896E-4	0.0	0.0	0.0036462895279348907	0.0
19	1.9603707139434896E-4	0.0	0.0	0.004116778499281328	0.0
20	1.9603707139434896E-4	0.0	0.0	0.006626053013128995	0.0
21	2.3524448567321875E-4	0.0	0.0	0.010468379612458235	3.9207414278869794E-5
22	2.3524448567321875E-4	0.0	0.0	0.017760958668328015	3.9207414278869794E-5
23	2.3524448567321875E-4	0.0	0.0	0.021564077853378386	3.9207414278869794E-5
24	2.3524448567321875E-4	0.0	0.0	0.028817449494969297	3.9207414278869794E-5
25	2.3524448567321875E-4	0.0	0.0	0.03399282817978011	3.9207414278869794E-5
26	2.3524448567321875E-4	0.0	0.0	0.04316736312103564	3.9207414278869794E-5
27	2.3524448567321875E-4	0.0	0.0	0.09456828324063393	3.9207414278869794E-5
28	2.3524448567321875E-4	0.0	0.0	0.19874238297959096	3.9207414278869794E-5
29	2.3524448567321875E-4	0.0	0.0	0.326401723871591	3.9207414278869794E-5
30	2.3524448567321875E-4	0.0	0.0	0.5046386291833331	3.9207414278869794E-5
31	2.3524448567321875E-4	0.0	0.0	0.7402359815850617	7.841482855773959E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7225	0.0	23.710726	1
CGCGAAT	70	1.2199373E-4	18.5	35
GTATTGG	855	0.0	17.309942	1
CAATGCG	130	1.3953468E-8	17.076923	19
ACGTTTA	120	1.0426447E-7	16.958334	26
TGCGCTA	635	0.0	16.606298	10
GCGCTAT	645	0.0	16.348837	11
CGCTATC	685	0.0	15.934306	12
ATTGGAC	900	0.0	15.827778	3
GTATCAA	11255	0.0	15.237227	2
TAATACT	745	0.0	15.147651	4
TTGGACC	1065	0.0	15.112676	4
TTGTGCG	700	0.0	14.799999	7
GCTATCG	705	0.0	14.695036	13
GACGCGA	90	8.278891E-4	14.388888	33
TGCGACG	155	1.2118653E-7	14.322581	22
GGACCCT	1150	0.0	14.31739	6
GTGCGCT	725	0.0	14.289656	9
CTAATAC	625	0.0	14.208001	3
TATCGGT	800	0.0	14.106249	15
>>END_MODULE