FastQCFastQC Report
Wed 25 May 2016
SRR2088692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634562
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT477171.029590282749481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT462460.9978504980621685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT352740.7611075221347778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167490.3613933743900718No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA152260.3285315850775111No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG152210.3284237000173911No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG106630.23007567921197297No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA105860.22841424928612455No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA98210.21190783508776018No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA87860.18957562764291425No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG85280.18400875854072077No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA65270.14083315748068534No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG61560.13282808601977922No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC60450.13043303768511458No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA54400.11737894541059113No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC52650.11360296830639012No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG52150.11252411770518983No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50890.1098054141901651No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG49850.10756140493966852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47980.10352650369117944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119650.026.903471
GTATTGG17850.020.8319321
ATTGGAC17850.019.380953
TTGGACC23750.019.1621064
GGACCCT25600.017.1269536
GTATCAA190100.016.9429262
ATCGTAC1005.885531E-616.65000225
TGGACCC27450.016.3096545
GACCCTC27100.015.5645767
TATTGGA23600.015.2076272
TAACGTG1001.0945006E-414.85
TTAACGG5400.014.38888835
ATACGAC2450.014.34693819
GTATTAG11100.013.51
GTCGTCA3200.013.29687524
ACCCTCG32600.013.2223928
CCCTCGC30750.013.05528459
TAGGACC6850.012.9635044
TCTAGAC4600.012.8695653
CTAATAC11400.012.8201763
Produced by FastQC (version 0.11.5)