##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4634562
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.586078900228326	31.0	31.0	34.0	30.0	34.0
2	31.792023496503013	31.0	31.0	34.0	30.0	34.0
3	31.87026346826302	31.0	31.0	34.0	30.0	34.0
4	35.587293038694916	37.0	35.0	37.0	33.0	37.0
5	35.46542175938093	37.0	35.0	37.0	33.0	37.0
6	35.503348536496006	37.0	35.0	37.0	33.0	37.0
7	35.372604789837744	37.0	35.0	37.0	33.0	37.0
8	35.39694754326299	37.0	35.0	37.0	33.0	37.0
9	36.998639569391884	39.0	37.0	39.0	33.0	39.0
10	36.811751142826445	39.0	37.0	39.0	32.0	39.0
11	36.900945332050796	39.0	37.0	39.0	33.0	39.0
12	36.75461240997531	39.0	37.0	39.0	32.0	39.0
13	36.790406083681695	39.0	37.0	39.0	32.0	39.0
14	37.788608071269735	40.0	37.0	41.0	32.0	41.0
15	37.794400420147575	40.0	37.0	41.0	32.0	41.0
16	37.68218571679481	39.0	37.0	41.0	32.0	41.0
17	37.69921213698296	39.0	37.0	41.0	32.0	41.0
18	37.65166589636734	39.0	37.0	41.0	32.0	41.0
19	37.69245205911584	40.0	37.0	41.0	32.0	41.0
20	37.56975761679313	39.0	37.0	41.0	32.0	41.0
21	37.50871236591505	39.0	36.0	41.0	32.0	41.0
22	37.3687047449144	39.0	36.0	41.0	31.0	41.0
23	37.19449518638439	39.0	36.0	41.0	31.0	41.0
24	37.040511918925674	39.0	36.0	41.0	31.0	41.0
25	37.24357468947443	39.0	36.0	41.0	31.0	41.0
26	37.13015404691964	39.0	36.0	41.0	31.0	41.0
27	37.026190177194735	39.0	36.0	41.0	31.0	41.0
28	36.861608712970074	39.0	36.0	41.0	30.0	41.0
29	36.75557409740122	39.0	35.0	41.0	30.0	41.0
30	36.57830535010644	39.0	35.0	40.0	30.0	41.0
31	36.43599179383079	39.0	35.0	40.0	30.0	41.0
32	36.28509166562018	38.0	35.0	40.0	30.0	41.0
33	36.156804893321095	38.0	35.0	40.0	30.0	41.0
34	35.9758400038666	38.0	35.0	40.0	29.0	41.0
35	35.82886904954557	38.0	35.0	40.0	28.0	41.0
36	35.654017790677955	38.0	35.0	40.0	27.0	41.0
37	35.60137160750034	38.0	34.0	40.0	27.0	41.0
38	35.426384844997216	38.0	34.0	40.0	26.0	41.0
39	35.353765684869465	38.0	34.0	40.0	25.0	41.0
40	35.191260576511866	38.0	34.0	40.0	25.0	41.0
41	35.07488582524088	38.0	34.0	40.0	24.0	41.0
42	34.81541340907727	38.0	33.0	40.0	23.0	41.0
43	34.48262683722863	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	10.0
15	25.0
16	40.0
17	115.0
18	297.0
19	780.0
20	1669.0
21	3296.0
22	6433.0
23	10927.0
24	17775.0
25	27385.0
26	39997.0
27	56437.0
28	75303.0
29	98995.0
30	125398.0
31	154975.0
32	190794.0
33	234014.0
34	288603.0
35	354121.0
36	455697.0
37	628778.0
38	863234.0
39	999458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.584576924421334	21.090795635056775	13.286649310118195	24.037978130403694
2	18.92703129227746	22.85970065779679	34.90757055359276	23.305697496332986
3	19.55550492150067	24.11541802655785	32.792893913168065	23.536183138773417
4	13.522162396360219	16.001123730786208	36.516244685042516	33.96046918781106
5	12.866027037722228	38.0350721384243	35.388953691848336	13.709947132005137
6	32.88025923485326	36.94513095304367	13.738428787876828	16.436181024226237
7	26.687958862131957	32.57399512618452	21.353474179437022	19.3845718322465
8	27.104869888459792	33.83124446279929	19.159890406040528	19.90399524270039
9	24.602540650011804	14.097276074848065	21.535282082751294	39.76490119238884
10	15.988414870704071	27.394562851894094	34.3750498968403	22.241972380561528
11	33.552512621473184	21.977092980954836	23.63181245606381	20.83858194150817
12	21.38068279159929	26.468391187775676	30.923634207504396	21.227291813120637
13	31.688992401007905	20.398605089326672	26.247960432938434	21.664442076726992
14	20.90279512929161	22.363645151364896	27.387895555178677	29.345664164164813
15	23.040062901305454	29.18707312578837	24.910228841474126	22.862635131432054
16	22.514856851629126	27.90464341614159	26.29381158348944	23.286688148739838
17	22.036710265177163	26.375437419976254	26.845147394726837	24.742704920119742
18	23.349261483609453	25.317948060679736	29.12842680710712	22.204363648603685
19	22.869129812051277	24.381052621585383	30.290499943683997	22.45931762267934
20	24.026024465742392	24.145237457175025	29.941966468460237	21.886771608622347
21	23.065761122626043	24.84077243976885	30.471789998709696	21.621676438895413
22	21.89305051912133	25.401710021356926	30.019794750830826	22.68544470869092
23	22.966442136279543	25.44072125909633	30.646628527140212	20.946208077483913
24	22.726441031536528	25.33139053917069	28.525716130240568	23.41645229905221
25	22.860434276205606	25.51024239183767	29.586118386160333	22.043204945796386
26	22.515353122905683	26.168859106858427	29.50067341854527	21.815114351690625
27	21.27745836607645	26.419864487733687	29.457519394497254	22.845157751692607
28	22.232046955030484	25.827208698470322	29.29737481125509	22.643369535244105
29	22.174997335239016	26.08054439664417	28.761703910747123	22.982754357369693
30	22.341766061172557	26.297544406569596	30.1309595167785	21.229730015479348
31	23.16609422853767	25.915652870756716	29.74291853253878	21.17533436816683
32	22.63588231207178	25.801942017390207	28.901652410734822	22.660523259803192
33	22.381036223056245	25.807552040516452	29.41076200944124	22.400649726986067
34	21.705028436344147	26.57409696968128	29.8542990686067	21.866575525367875
35	21.26576362555944	28.650927531015874	28.89643077382501	21.186878069599675
36	23.31929101390811	26.44795775738894	28.686788524999773	21.545962703703175
37	22.355122231615415	27.211848714074815	28.689205150346464	21.743823903963307
38	22.31168770641109	26.029406878147277	29.551638320945973	22.10726709449566
39	21.958709366710384	25.788672154995446	30.33156099756568	21.921057480728493
40	20.855304125826777	26.571572459274467	29.830844856536604	22.742278558362152
41	22.57067658173523	25.397222002855933	29.766027512416493	22.266073902992343
42	20.994130621189232	27.31723084080006	29.342557074433355	22.346081463577356
43	21.482979405605104	27.121786265886616	28.53061411197002	22.864620216538263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	874.0
1	908.0
2	942.0
3	1730.0
4	2518.0
5	2518.0
6	4164.0
7	5810.0
8	6067.0
9	6324.0
10	10080.0
11	13836.0
12	13836.0
13	26944.0
14	40052.0
15	65489.5
16	90927.0
17	85823.0
18	80719.0
19	80719.0
20	89870.5
21	99022.0
22	81835.5
23	64649.0
24	73622.5
25	82596.0
26	82596.0
27	92963.5
28	103331.0
29	111610.0
30	119889.0
31	132260.0
32	144631.0
33	144631.0
34	160134.5
35	175638.0
36	191477.5
37	207317.0
38	216602.0
39	225887.0
40	225887.0
41	231834.0
42	237781.0
43	250751.0
44	263721.0
45	278927.5
46	294134.0
47	294134.0
48	374467.0
49	454800.0
50	434749.5
51	414699.0
52	387097.0
53	359495.0
54	359495.0
55	327427.5
56	295360.0
57	256498.5
58	217637.0
59	197407.5
60	177178.0
61	177178.0
62	156934.0
63	136690.0
64	119988.0
65	103286.0
66	88226.0
67	73166.0
68	73166.0
69	62356.0
70	51546.0
71	44204.0
72	36862.0
73	29925.5
74	22989.0
75	22989.0
76	18409.0
77	13829.0
78	10783.5
79	7738.0
80	6023.5
81	4309.0
82	4309.0
83	3260.0
84	2211.0
85	1653.5
86	1096.0
87	836.5
88	577.0
89	577.0
90	435.0
91	293.0
92	213.0
93	133.0
94	92.5
95	52.0
96	52.0
97	32.5
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4634562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.3830914478334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29206856514392	30.330523758322265
2	13.897153098126344	11.502143150486074
3	5.130897775280068	6.369972355316998
4	2.4295044415887213	4.021616179167339
5	1.3506149909989706	2.794631184166254
6	0.7904149851261364	1.962588936672767
7	0.5307829843354596	1.537580854579577
8	0.36684799775350474	1.2145043390790293
9	0.2937905833739981	1.0942166320450637
>10	1.60783691071636	12.848113723663
>50	0.1792783212924041	5.1765500885794
>100	0.11658919368811305	9.103337773428786
>500	0.008469355710299335	2.356015752141707
>1k	0.004809757378575879	3.964561382271677
>5k	5.227997150625928E-4	1.446780295204128
>10k+	4.1823977205007425E-4	4.276863594875919
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47717	1.029590282749481	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46246	0.9978504980621685	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35274	0.7611075221347778	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16749	0.3613933743900718	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	15226	0.3285315850775111	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15221	0.3284237000173911	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10663	0.23007567921197297	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10586	0.22841424928612455	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9821	0.21190783508776018	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8786	0.18957562764291425	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8528	0.18400875854072077	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6527	0.14083315748068534	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	6156	0.13282808601977922	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6045	0.13043303768511458	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5440	0.11737894541059113	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	5265	0.11360296830639012	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5215	0.11252411770518983	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5089	0.1098054141901651	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4985	0.10756140493966852	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4798	0.10352650369117944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.157701202400572E-5	0.0	0.0	0.0	0.0
3	2.157701202400572E-5	0.0	0.0	0.0	0.0
4	4.315402404801144E-5	0.0	0.0	0.0	0.0
5	4.315402404801144E-5	0.0	0.0	0.0	0.0
6	6.473103607201716E-5	0.0	0.0	0.0	0.0
7	6.473103607201716E-5	0.0	0.0	0.0	0.0
8	8.630804809602288E-5	0.0	0.0	2.157701202400572E-5	0.0
9	8.630804809602288E-5	0.0	0.0	2.157701202400572E-5	0.0
10	1.7261609619204576E-4	0.0	0.0	2.157701202400572E-5	0.0
11	1.7261609619204576E-4	0.0	0.0	4.315402404801144E-5	0.0
12	1.7261609619204576E-4	0.0	0.0	4.315402404801144E-5	0.0
13	1.941931082160515E-4	0.0	0.0	4.315402404801144E-5	0.0
14	2.3734713226406293E-4	0.0	0.0	6.473103607201716E-5	0.0
15	2.3734713226406293E-4	0.0	0.0	1.2946207214403432E-4	0.0
16	2.3734713226406293E-4	0.0	0.0	4.099632284561087E-4	0.0
17	2.3734713226406293E-4	0.0	0.0	5.610023126241487E-4	0.0
18	2.3734713226406293E-4	0.0	0.0	6.688873727441774E-4	0.0
19	2.5892414428806864E-4	0.0	0.0	9.062345050082403E-4	0.0
20	2.5892414428806864E-4	0.0	0.0	0.0014456598056083834	0.0
21	2.5892414428806864E-4	0.0	0.0	0.002610818454904692	0.0
22	2.8050115631207434E-4	0.0	0.0	0.004466441488969184	0.0
23	2.8050115631207434E-4	0.0	0.0	0.005610023126241487	0.0
24	3.020781683360801E-4	0.0	0.0	0.007185145003993905	0.0
25	3.020781683360801E-4	0.0	0.0	0.008285572617218196	0.0
26	3.236551803600858E-4	0.0	0.0	0.011435816372723033	0.0
27	3.452321923840915E-4	0.0	0.0	0.028416924835615535	0.0
28	3.452321923840915E-4	0.0	0.0	0.06956428676539445	0.0
29	3.452321923840915E-4	0.0	0.0	0.13067038481737864	0.0
30	3.452321923840915E-4	0.0	0.0	0.21887720997151403	0.0
31	3.452321923840915E-4	0.0	0.0	0.32253317573483753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11965	0.0	26.90347	1
GTATTGG	1785	0.0	20.831932	1
ATTGGAC	1785	0.0	19.38095	3
TTGGACC	2375	0.0	19.162106	4
GGACCCT	2560	0.0	17.126953	6
GTATCAA	19010	0.0	16.942926	2
ATCGTAC	100	5.885531E-6	16.650002	25
TGGACCC	2745	0.0	16.309654	5
GACCCTC	2710	0.0	15.564576	7
TATTGGA	2360	0.0	15.207627	2
TAACGTG	100	1.0945006E-4	14.8	5
TTAACGG	540	0.0	14.388888	35
ATACGAC	245	0.0	14.346938	19
GTATTAG	1110	0.0	13.5	1
GTCGTCA	320	0.0	13.296875	24
ACCCTCG	3260	0.0	13.222392	8
CCCTCGC	3075	0.0	13.0552845	9
TAGGACC	685	0.0	12.963504	4
TCTAGAC	460	0.0	12.869565	3
CTAATAC	1140	0.0	12.820176	3
>>END_MODULE