FastQCFastQC Report
Wed 25 May 2016
SRR2088691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179748
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT350231.1014394851415898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT331621.0429128346019874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT249560.784842069245739No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133290.41918416176376244No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81460.2561838233721666No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG77480.24366710821109092No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72280.2273136110157157No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA66550.20929331506773494No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61570.19363169659985635No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56390.17734109747061716No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA43660.13730647837501586No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.12312296446133468No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC39020.12271412703145029No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.11831126240192619No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA36260.1140341939046742No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34920.10982002347355829No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32160.1011400903467822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94500.025.1756611
GTATTGG8800.019.3409081
TACGTTA852.7255657E-517.41176419
CTCTAAT6150.016.5447161
ATTGGAC10350.016.0869563
GTATCAA148600.015.9602962
GTATTAG6650.015.8571421
CTAATAC7600.015.3355263
TTGGACC14350.015.21254354
GGACCCT13650.015.1794876
ATACTGG6500.014.86
TATACCG2251.8189894E-1214.85
TTATGCG3400.014.6911764
TCTAATA7950.014.6603772
TACTGGT6200.014.6209687
TAATACT8250.014.5757564
TGGACCC14900.014.5268455
GTACTAG4300.014.1976741
TATGCGG3700.014.05
TAGACTG4000.013.8749995
Produced by FastQC (version 0.11.5)