##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3179748
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59518490144502	31.0	31.0	34.0	30.0	34.0
2	31.799328122857535	31.0	31.0	34.0	30.0	34.0
3	31.86859398920921	31.0	31.0	34.0	30.0	34.0
4	35.587701918516814	37.0	35.0	37.0	33.0	37.0
5	35.46028018572541	37.0	35.0	37.0	33.0	37.0
6	35.509284383542344	37.0	35.0	37.0	33.0	37.0
7	35.376642111261646	37.0	35.0	37.0	33.0	37.0
8	35.408371355214314	37.0	35.0	37.0	33.0	37.0
9	37.00368079483028	39.0	37.0	39.0	33.0	39.0
10	36.82240447985186	39.0	37.0	39.0	32.0	39.0
11	36.91427056483721	39.0	37.0	39.0	33.0	39.0
12	36.768991442089124	39.0	37.0	39.0	32.0	39.0
13	36.81370127444062	39.0	37.0	39.0	32.0	39.0
14	37.81334401342497	40.0	37.0	41.0	32.0	41.0
15	37.81894540070471	40.0	37.0	41.0	32.0	41.0
16	37.71020816743968	39.0	37.0	41.0	32.0	41.0
17	37.73536173306815	39.0	37.0	41.0	32.0	41.0
18	37.689064353527385	39.0	37.0	41.0	32.0	41.0
19	37.741662232353	40.0	37.0	41.0	32.0	41.0
20	37.619618598706566	39.0	37.0	41.0	32.0	41.0
21	37.56032396277944	39.0	36.0	41.0	32.0	41.0
22	37.431685781388964	39.0	36.0	41.0	32.0	41.0
23	37.26699694441195	39.0	36.0	41.0	31.0	41.0
24	37.10650812580116	39.0	36.0	41.0	31.0	41.0
25	37.291085016800075	39.0	36.0	41.0	31.0	41.0
26	37.179220177196434	39.0	36.0	41.0	31.0	41.0
27	37.075188977239705	39.0	36.0	41.0	31.0	41.0
28	36.901555091787145	39.0	35.0	41.0	30.0	41.0
29	36.7946853021057	39.0	35.0	41.0	30.0	41.0
30	36.600330906725944	39.0	35.0	40.0	30.0	41.0
31	36.447273809119466	39.0	35.0	40.0	30.0	41.0
32	36.28122354350093	38.0	35.0	40.0	30.0	41.0
33	36.14843440423581	38.0	35.0	40.0	30.0	41.0
34	35.977490354581555	38.0	35.0	40.0	29.0	41.0
35	35.85020919896797	38.0	35.0	40.0	28.0	41.0
36	35.657730109430055	38.0	35.0	40.0	27.0	41.0
37	35.60356827018997	38.0	35.0	40.0	27.0	41.0
38	35.41796016539676	38.0	34.0	40.0	26.0	41.0
39	35.327388837102816	38.0	34.0	40.0	25.0	41.0
40	35.16433409188401	38.0	34.0	40.0	24.0	41.0
41	35.03955376337999	38.0	34.0	40.0	24.0	41.0
42	34.791526246734016	38.0	34.0	40.0	23.0	41.0
43	34.44349457881568	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	5.0
14	3.0
15	13.0
16	39.0
17	77.0
18	220.0
19	477.0
20	1037.0
21	2201.0
22	4196.0
23	7479.0
24	12064.0
25	18500.0
26	27123.0
27	37852.0
28	51566.0
29	67458.0
30	85384.0
31	105958.0
32	130737.0
33	160499.0
34	197285.0
35	242952.0
36	312659.0
37	430285.0
38	587882.0
39	695790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80474301737119	21.58718238049053	14.182318850424625	23.42575575171366
2	18.97817059716682	23.26426496690933	34.99082317215075	22.766741263773106
3	19.63004615460093	24.364635184926602	32.75423083841864	23.251087822053822
4	13.933242508525833	16.627575518563106	36.03879930107669	33.40038267183437
5	12.64609648311753	38.58019566330413	35.05101662144296	13.722691232135375
6	32.34190256586371	37.263047260348934	13.860312200841074	16.534737972946285
7	25.893592825594986	33.19354238134594	21.65067797825488	19.262186814804192
8	26.63987838029932	34.82823167118904	19.13519561927549	19.39669432923615
9	24.427376005897322	14.648833806955771	22.060427430098233	38.86336275704867
10	16.339376579527688	27.932527986494527	33.84494620328403	21.883149230693753
11	33.000350971209045	22.6688718728654	23.514976658527655	20.81580049739791
12	20.72267991048347	27.342386880972956	31.103282398479376	20.831650810064197
13	31.025446041636002	21.341848473526834	26.293341484922706	21.33936399991446
14	20.26581980710421	23.3352139855108	27.959322562668486	28.4396436447165
15	22.69915729170991	29.8167968027655	25.46346440032355	22.020581505201044
16	21.716406457367064	28.556917089027177	26.8155841280504	22.91109232555536
17	21.399117162743714	27.35436896257188	27.325184259884743	23.921329614799664
18	22.666230154087682	26.086375398302003	29.54152341632104	21.705871031289274
19	22.477315812448033	25.3946539159707	30.801591824257773	21.326438447323497
20	23.380091755698878	24.98993630941823	30.457759545725004	21.17221238915788
21	22.266607290892235	25.753172892946235	31.06714745948421	20.91307235667732
22	21.158217569442613	26.103766713588623	30.56028339352678	22.177732323441983
23	22.031179829344964	26.225788961892576	31.196591679592217	20.546439529170236
24	21.890445406365536	26.437315158308145	29.22335354877179	22.44888588655453
25	21.923498340120034	26.539839006109915	30.24783724999591	21.288825403774137
26	21.539002461830307	27.253291770291234	30.111788732943616	21.095917034934843
27	20.667046570986127	27.221528246892525	29.855321868273837	22.25610331384751
28	21.123340591770166	26.724979463781406	30.10345473918059	22.048225205267837
29	21.373140261429523	26.639846931266252	29.541083129858087	22.445929677446134
30	21.550544256966276	26.911252086643344	30.75919852768207	20.779005128708313
31	22.149019356250875	26.676673748988915	30.533551715418955	20.640755179341255
32	21.737288615324232	26.499175406352954	29.799216793280475	21.964319185042335
33	21.28763034051755	26.387059603465428	30.411560916148073	21.913749139868948
34	20.70261542738607	26.682711963337972	30.993305129840476	21.621367479435477
35	20.403692368074452	28.32152107651298	30.028763285644022	21.246023269768546
36	22.220503008414504	26.36737250876485	29.89815545131249	21.513969031508157
37	21.34508772393284	26.771586930788228	30.092950762135867	21.790374583143066
38	21.086922611477387	25.706785569170894	31.25820033537249	21.948091483979233
39	20.68770858571182	25.5231232160536	31.827144792606205	21.96202340562837
40	19.77672444483022	25.86242683382457	31.67927143912033	22.68157728222488
41	20.80727780943647	25.094284201137953	31.679680276550215	22.418757712875358
42	19.71813489622448	26.828887069038178	31.060448815440722	22.392529219296623
43	20.03608462054226	26.636198843430357	30.463011534247368	22.864705001780013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1170.0
1	1053.5
2	937.0
3	1645.5
4	2354.0
5	2354.0
6	3643.5
7	4933.0
8	5381.0
9	5829.0
10	9162.0
11	12495.0
12	12495.0
13	23434.5
14	34374.0
15	53508.0
16	72642.0
17	67545.5
18	62449.0
19	62449.0
20	68742.5
21	75036.0
22	61337.5
23	47639.0
24	53507.5
25	59376.0
26	59376.0
27	65670.5
28	71965.0
29	78423.0
30	84881.0
31	93469.5
32	102058.0
33	102058.0
34	113930.5
35	125803.0
36	136590.0
37	147377.0
38	156756.0
39	166135.0
40	166135.0
41	171793.5
42	177452.0
43	187995.5
44	198539.0
45	208941.0
46	219343.0
47	219343.0
48	272243.5
49	325144.0
50	301905.5
51	278667.0
52	252239.5
53	225812.0
54	225812.0
55	203560.5
56	181309.0
57	157058.5
58	132808.0
59	119797.0
60	106786.0
61	106786.0
62	94159.5
63	81533.0
64	70748.0
65	59963.0
66	50265.5
67	40568.0
68	40568.0
69	34550.5
70	28533.0
71	23712.0
72	18891.0
73	15353.5
74	11816.0
75	11816.0
76	9372.0
77	6928.0
78	5360.0
79	3792.0
80	2960.0
81	2128.0
82	2128.0
83	1620.0
84	1112.0
85	846.5
86	581.0
87	438.5
88	296.0
89	296.0
90	250.5
91	205.0
92	130.0
93	55.0
94	38.0
95	21.0
96	21.0
97	15.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3179748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.290782192365505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69766771655988	37.520811485551825
2	12.382162577977827	11.958886322471724
3	4.136133899461203	5.992114237720212
4	1.8421859593327254	3.5584240367988214
5	0.9836260259030749	2.375003508781373
6	0.5878282484818508	1.7032011548374062
7	0.3963243404579989	1.3397168681813094
8	0.28316236726673144	1.0939305762201867
9	0.20773981058604918	0.9028726151124749
>10	1.261630407632875	11.630787493100698
>50	0.1287629224773499	4.348439933624757
>100	0.08253300697053582	7.497103901297695
>500	0.0056759004434590405	1.8094593058531314
>1k	0.0039144140986902285	3.6051509178839827
>5k	3.9144140986902286E-4	1.309758187110056
>10k+	2.6096093991268194E-4	3.3543394554544457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35023	1.1014394851415898	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33162	1.0429128346019874	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24956	0.784842069245739	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13329	0.41918416176376244	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8146	0.2561838233721666	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7748	0.24366710821109092	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7228	0.2273136110157157	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6655	0.20929331506773494	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6157	0.19363169659985635	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5639	0.17734109747061716	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4366	0.13730647837501586	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3915	0.12312296446133468	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3902	0.12271412703145029	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3762	0.11831126240192619	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3626	0.1140341939046742	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3492	0.10982002347355829	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3216	0.1011400903467822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.144903306802929E-5	0.0	0.0	0.0	0.0
3	3.144903306802929E-5	0.0	0.0	0.0	0.0
4	3.144903306802929E-5	0.0	0.0	0.0	0.0
5	3.144903306802929E-5	0.0	0.0	0.0	0.0
6	3.144903306802929E-5	0.0	0.0	3.144903306802929E-5	0.0
7	3.144903306802929E-5	0.0	3.144903306802929E-5	3.144903306802929E-5	0.0
8	3.144903306802929E-5	0.0	3.144903306802929E-5	3.144903306802929E-5	0.0
9	3.144903306802929E-5	0.0	3.144903306802929E-5	1.5724516534014644E-4	0.0
10	3.144903306802929E-5	0.0	3.144903306802929E-5	2.515922645442343E-4	0.0
11	6.289806613605858E-5	0.0	3.144903306802929E-5	3.459393637483222E-4	0.0
12	6.289806613605858E-5	0.0	3.144903306802929E-5	5.34633562156498E-4	0.0
13	6.289806613605858E-5	0.0	3.144903306802929E-5	5.660825952245272E-4	0.0
14	6.289806613605858E-5	0.0	3.144903306802929E-5	6.918787274966444E-4	0.0
15	6.289806613605858E-5	0.0	3.144903306802929E-5	9.120219589728494E-4	0.0
16	6.289806613605858E-5	0.0	3.144903306802929E-5	0.0011636142235170838	0.0
17	6.289806613605858E-5	0.0	3.144903306802929E-5	0.0017925948848776696	0.0
18	6.289806613605858E-5	0.0	3.144903306802929E-5	0.0022014323147620503	0.0
19	6.289806613605858E-5	0.0	3.144903306802929E-5	0.0030820052406668706	0.0
20	6.289806613605858E-5	0.0	3.144903306802929E-5	0.005189090456224833	0.0
21	6.289806613605858E-5	0.0	3.144903306802929E-5	0.008491238928367909	0.0
22	6.289806613605858E-5	0.0	3.144903306802929E-5	0.013931921649136975	0.0
23	6.289806613605858E-5	0.0	3.144903306802929E-5	0.01776870368343655	0.0
24	6.289806613605858E-5	0.0	3.144903306802929E-5	0.02393271416477029	0.0
25	6.289806613605858E-5	0.0	3.144903306802929E-5	0.02890166138951892	0.0
26	9.434709920408787E-5	0.0	6.289806613605858E-5	0.039216944235832524	0.0
27	9.434709920408787E-5	0.0	6.289806613605858E-5	0.09878141286668	0.0
28	9.434709920408787E-5	0.0	6.289806613605858E-5	0.21608630621042926	0.0
29	9.434709920408787E-5	0.0	6.289806613605858E-5	0.36584660168038474	0.0
30	9.434709920408787E-5	0.0	9.434709920408787E-5	0.5930344165638284	0.0
31	9.434709920408787E-5	0.0	9.434709920408787E-5	0.8920203739415828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9450	0.0	25.175661	1
GTATTGG	880	0.0	19.340908	1
TACGTTA	85	2.7255657E-5	17.411764	19
CTCTAAT	615	0.0	16.544716	1
ATTGGAC	1035	0.0	16.086956	3
GTATCAA	14860	0.0	15.960296	2
GTATTAG	665	0.0	15.857142	1
CTAATAC	760	0.0	15.335526	3
TTGGACC	1435	0.0	15.2125435	4
GGACCCT	1365	0.0	15.179487	6
ATACTGG	650	0.0	14.8	6
TATACCG	225	1.8189894E-12	14.8	5
TTATGCG	340	0.0	14.691176	4
TCTAATA	795	0.0	14.660377	2
TACTGGT	620	0.0	14.620968	7
TAATACT	825	0.0	14.575756	4
TGGACCC	1490	0.0	14.526845	5
GTACTAG	430	0.0	14.197674	1
TATGCGG	370	0.0	14.0	5
TAGACTG	400	0.0	13.874999	5
>>END_MODULE