FastQCFastQC Report
Wed 25 May 2016
SRR2088690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489994
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT452991.2979678475091934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT416481.1933544871423847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT326450.9353884276018811No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168220.4820065593235977No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG113450.32507219210118987No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG107960.3093415060312425No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA99010.28369676280245754No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95290.27303771868948773No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG87250.25000042980016585No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82840.23736430492430644No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA59890.1716048795499362No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC57750.1654730638505396No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA53740.15398307275026835No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA49060.14057330757588696No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47720.13673375942766663No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.12280823405427058No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA42440.12160479358990302No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41760.11965636617140316No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.11295148358421248No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35420.10149014582833095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116200.024.8205681
GTATTGG12200.020.4713121
ATTGGAC12150.019.4897123
CGTATCG1254.129106E-1019.2410
GTATTAG6450.017.2093011
TTGGACC18600.017.0080664
TGGACCC19100.016.2722515
ACGTTTA1606.311893E-1016.187526
GGACCCT19550.015.708446
TTAACGG3700.015.535
GTATCAA187300.015.4084352
GACCCTC19650.015.3460557
CTAATAC8100.014.6172843
TATTGGA16800.014.3154752
TAACGGC4050.014.16049436
CGCACTA950.001245963213.63157929
TAATACT8700.013.6091954
TAGGTCG1805.1686584E-813.36111121
AGTACCG1951.0280928E-813.2820525
TCTAACG1551.8904593E-613.1290322
Produced by FastQC (version 0.11.5)