##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3489994
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569009574228495	31.0	31.0	34.0	30.0	34.0
2	31.772348032690026	31.0	31.0	34.0	30.0	34.0
3	31.83828854720094	31.0	31.0	34.0	30.0	34.0
4	35.560481192804346	37.0	35.0	37.0	33.0	37.0
5	35.4432729683776	37.0	35.0	37.0	33.0	37.0
6	35.49472692503196	37.0	35.0	37.0	33.0	37.0
7	35.35261063486069	37.0	35.0	37.0	33.0	37.0
8	35.39022932417649	37.0	35.0	37.0	33.0	37.0
9	36.978056695799474	39.0	37.0	39.0	33.0	39.0
10	36.800228882914986	39.0	37.0	39.0	32.0	39.0
11	36.88838089692991	39.0	37.0	39.0	33.0	39.0
12	36.74365228135063	39.0	35.0	39.0	32.0	39.0
13	36.79149820887944	39.0	37.0	39.0	32.0	39.0
14	37.75453510808328	40.0	37.0	41.0	32.0	41.0
15	37.76782338307745	40.0	37.0	41.0	32.0	41.0
16	37.65470370436167	39.0	36.0	41.0	32.0	41.0
17	37.70118430003031	39.0	37.0	41.0	32.0	41.0
18	37.66325013739279	39.0	37.0	41.0	32.0	41.0
19	37.71849980257846	40.0	37.0	41.0	32.0	41.0
20	37.59791965258393	39.0	37.0	41.0	32.0	41.0
21	37.54107313651542	39.0	36.0	41.0	32.0	41.0
22	37.412267470946944	39.0	36.0	41.0	32.0	41.0
23	37.23357661932943	39.0	36.0	41.0	31.0	41.0
24	37.07534310947239	39.0	36.0	41.0	31.0	41.0
25	37.25342937552328	39.0	36.0	41.0	31.0	41.0
26	37.12740623622849	39.0	36.0	41.0	31.0	41.0
27	37.01091148007704	39.0	36.0	41.0	31.0	41.0
28	36.83481060425892	39.0	35.0	41.0	30.0	41.0
29	36.7158003710035	39.0	35.0	41.0	30.0	41.0
30	36.5091951447481	39.0	35.0	40.0	30.0	41.0
31	36.32782807076459	38.0	35.0	40.0	30.0	41.0
32	36.15707849354469	38.0	35.0	40.0	30.0	41.0
33	36.01872266829112	38.0	35.0	40.0	29.0	41.0
34	35.82793380160539	38.0	35.0	40.0	29.0	41.0
35	35.69039201786593	38.0	35.0	40.0	27.0	41.0
36	35.48459281018821	38.0	34.0	40.0	27.0	41.0
37	35.40272160926351	38.0	34.0	40.0	26.0	41.0
38	35.20019604618231	38.0	34.0	40.0	25.0	41.0
39	35.100720516998024	38.0	34.0	40.0	25.0	41.0
40	34.91605974107692	38.0	34.0	40.0	24.0	41.0
41	34.76836435821953	38.0	33.0	40.0	23.0	41.0
42	34.489008290558665	38.0	33.0	40.0	22.0	41.0
43	34.12845638130037	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	1.0
14	6.0
15	17.0
16	40.0
17	83.0
18	241.0
19	550.0
20	1250.0
21	2521.0
22	4904.0
23	8631.0
24	13869.0
25	21477.0
26	31326.0
27	43734.0
28	59442.0
29	76435.0
30	97063.0
31	119824.0
32	147072.0
33	180919.0
34	221138.0
35	270792.0
36	346899.0
37	477924.0
38	630466.0
39	733364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98256329380509	21.483360716379455	14.391314139795083	23.14276185002037
2	18.820089662045262	23.523536143615146	35.09281104781269	22.5635631465269
3	19.619775850617508	24.40353765651173	33.181575670330666	22.795110822540096
4	13.621255509321792	16.45994233800975	36.144188213504094	33.77461393916437
5	12.351339285970118	38.70688602903042	35.11183686848745	13.829937816512006
6	32.20340779955496	37.239261729389796	13.574579211310963	16.982751259744287
7	25.560244516179687	33.57424110184717	21.42760130819709	19.43791307377606
8	26.939014794867845	34.5637556970012	19.04602128255808	19.45120822557288
9	24.26356606916803	14.57420843703456	22.104593876092622	39.057631617704786
10	16.160457582448565	28.063142801964702	33.763152601408486	22.013247014178248
11	32.70461209961966	22.538348203463958	23.77196063947388	20.985079057442505
12	20.947715096358333	27.48887247370626	31.143577897268592	20.41983453266682
13	31.540971130609393	21.22828864462231	26.475375029298043	20.755365195470247
14	20.207398637361553	23.302017138138346	28.231251973499095	28.259332251001005
15	22.25791792192193	29.9488480495955	25.9439701042466	21.849263924235974
16	21.272414794982456	28.73483450114814	27.101278684146735	22.89147201972267
17	21.299692778841454	27.155805998520343	27.359645890508695	24.18485533212951
18	22.73468091922221	25.877207811818586	30.00773067231634	21.38038059664286
19	22.267258912192972	24.98732089510756	31.39899380915841	21.34642638354106
20	23.363879708675718	24.580185524674256	31.071973189638726	20.983961577011307
21	22.09427867211233	25.532250198710944	31.803149231775187	20.57032189740154
22	20.714906673191987	25.839700584012466	31.24701647051542	22.198376272280125
23	21.819493099415073	26.063511857040446	32.06867977423457	20.048315269309917
24	21.728375464255812	26.05153475908555	29.742314743234516	22.477775033424127
25	21.524965372433304	26.507610041736463	30.878878301796508	21.088546284033725
26	21.301154099405327	27.114831716043064	30.73764596729966	20.84636821725195
27	20.201410088384105	27.14930168934388	30.46515266215357	22.184135560118442
28	20.735852267940864	26.696263661198273	30.600367794328587	21.96751627653228
29	21.053073443679274	26.56755283819972	29.906956860097754	22.47241685802325
30	21.21711384031033	26.856120669548428	31.53389375454514	20.392871735596106
31	22.041785745190392	26.565059997237817	31.130741198982005	20.262413058589786
32	21.549005528376266	26.45672170210035	30.223576315603978	21.770696453919406
33	21.066311288787315	26.390704396626468	30.95105607631417	21.591928238272043
34	20.227370018401178	26.957983308853827	31.63710310103685	21.177543571708146
35	19.84224041645917	28.975006833822636	30.342201161377353	20.840551588340837
36	22.0392642508841	26.635088770926252	30.21039577718472	21.11525120100493
37	21.175767064355984	27.37635079028789	30.21695739304996	21.230924752306166
38	20.98095297585039	25.92574657721475	31.455870697771974	21.63742974916289
39	20.52298657246975	25.70987228058272	32.225241647979914	21.54189949896762
40	19.397425898153404	26.161620908230788	31.87171668489974	22.569236508716063
41	20.844878243343683	25.317350115788162	31.826616320830354	22.0111553200378
42	19.542755660897985	27.274917951148343	31.206357374826432	21.975969013127244
43	19.935163212314976	27.16394354832702	30.396355982273892	22.50453725708411
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1293.0
1	1164.0
2	1035.0
3	1846.0
4	2657.0
5	2657.0
6	4257.0
7	5857.0
8	6481.0
9	7105.0
10	11101.5
11	15098.0
12	15098.0
13	28698.5
14	42299.0
15	66967.5
16	91636.0
17	84612.0
18	77588.0
19	77588.0
20	83564.0
21	89540.0
22	72147.0
23	54754.0
24	60929.0
25	67104.0
26	67104.0
27	73971.5
28	80839.0
29	86905.0
30	92971.0
31	102198.0
32	111425.0
33	111425.0
34	123058.5
35	134692.0
36	146567.0
37	158442.0
38	166332.5
39	174223.0
40	174223.0
41	178645.0
42	183067.0
43	192693.0
44	202319.0
45	217202.0
46	232085.0
47	232085.0
48	304173.5
49	376262.0
50	348009.5
51	319757.0
52	287686.5
53	255616.0
54	255616.0
55	226679.0
56	197742.0
57	168041.5
58	138341.0
59	122847.5
60	107354.0
61	107354.0
62	94951.5
63	82549.0
64	71930.0
65	61311.0
66	52486.5
67	43662.0
68	43662.0
69	37043.0
70	30424.0
71	25646.5
72	20869.0
73	16852.0
74	12835.0
75	12835.0
76	10325.0
77	7815.0
78	6098.5
79	4382.0
80	3414.0
81	2446.0
82	2446.0
83	1862.5
84	1279.0
85	970.0
86	661.0
87	512.5
88	364.0
89	364.0
90	264.0
91	164.0
92	126.0
93	88.0
94	58.0
95	28.0
96	28.0
97	20.5
98	13.0
99	8.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3489994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.25921482052065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46099283998709	36.60745700835761
2	12.536112492655008	11.84893666609191
3	4.2595661567314105	6.039112561295677
4	1.880951254448694	3.555691176036744
5	0.9689159735306452	2.2895104068059324
6	0.5824395162734677	1.6515380531716763
7	0.37879725091247524	1.2531162458006728
8	0.27119751454644286	1.025326527899329
9	0.20577020205389485	0.8752084364274259
>10	1.2404372709131486	11.184663178261335
>50	0.12473491634650623	4.084061296032358
>100	0.07882634365237606	7.048075850101849
>500	0.005659561726259646	1.7915650134573942
>1k	0.004807584691215297	4.644856767129349
>5k	4.2598851694312755E-4	1.540865552227418
>10k+	3.651330145226808E-4	4.56001526090334
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45299	1.2979678475091934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41648	1.1933544871423847	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32645	0.9353884276018811	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16822	0.4820065593235977	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11345	0.32507219210118987	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10796	0.3093415060312425	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9901	0.28369676280245754	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9529	0.27303771868948773	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8725	0.25000042980016585	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8284	0.23736430492430644	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5989	0.1716048795499362	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5775	0.1654730638505396	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5374	0.15398307275026835	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4906	0.14057330757588696	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4772	0.13673375942766663	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4286	0.12280823405427058	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4244	0.12160479358990302	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4176	0.11965636617140316	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3942	0.11295148358421248	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3542	0.10149014582833095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.8653344389703822E-5	0.0	0.0	0.0	0.0
6	2.8653344389703822E-5	0.0	0.0	0.0	0.0
7	2.8653344389703822E-5	0.0	0.0	0.0	0.0
8	5.7306688779407645E-5	0.0	0.0	0.0	0.0
9	8.596003316911147E-5	0.0	0.0	1.432667219485191E-4	0.0
10	8.596003316911147E-5	0.0	0.0	2.0057341072792675E-4	0.0
11	8.596003316911147E-5	0.0	0.0	5.157601990146688E-4	0.0
12	1.1461337755881529E-4	0.0	0.0	7.449869541322993E-4	0.0
13	1.7192006633822294E-4	0.0	0.0	9.169070204705223E-4	0.0
14	2.0057341072792675E-4	0.0	0.0	0.0010888270868087452	0.0
15	2.0057341072792675E-4	0.0	0.0	0.001289400497536672	0.0
16	2.0057341072792675E-4	0.0	0.0	0.00194842741849986	0.0
17	2.0057341072792675E-4	0.0	0.0	0.002578800995073344	0.0
18	2.0057341072792675E-4	0.0	0.0	0.0031232145384777164	0.0
19	2.2922675511763058E-4	0.0	0.0	0.004011468214558535	0.0
20	2.2922675511763058E-4	0.0	0.0	0.006504309176462768	0.0
21	2.2922675511763058E-4	0.0	0.0	0.010515777391021303	0.0
22	2.2922675511763058E-4	0.0	0.0	0.016017219513844435	0.0
23	2.2922675511763058E-4	0.0	0.0	0.01971350094011623	0.0
24	2.578800995073344E-4	0.0	0.0	0.026074543394630476	0.0
25	2.578800995073344E-4	0.0	0.0	0.030773691874541903	0.0
26	2.578800995073344E-4	0.0	0.0	0.03985680204607801	0.0
27	2.578800995073344E-4	0.0	0.0	0.0951577567182064	0.0
28	2.578800995073344E-4	0.0	0.0	0.20561639934051462	0.0
29	2.578800995073344E-4	0.0	0.0	0.3467627738041957	0.0
30	2.578800995073344E-4	0.0	0.0	0.5638691642449815	0.0
31	2.578800995073344E-4	0.0	0.0	0.8144140075885518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11620	0.0	24.820568	1
GTATTGG	1220	0.0	20.471312	1
ATTGGAC	1215	0.0	19.489712	3
CGTATCG	125	4.129106E-10	19.24	10
GTATTAG	645	0.0	17.209301	1
TTGGACC	1860	0.0	17.008066	4
TGGACCC	1910	0.0	16.272251	5
ACGTTTA	160	6.311893E-10	16.1875	26
GGACCCT	1955	0.0	15.70844	6
TTAACGG	370	0.0	15.5	35
GTATCAA	18730	0.0	15.408435	2
GACCCTC	1965	0.0	15.346055	7
CTAATAC	810	0.0	14.617284	3
TATTGGA	1680	0.0	14.315475	2
TAACGGC	405	0.0	14.160494	36
CGCACTA	95	0.0012459632	13.631579	29
TAATACT	870	0.0	13.609195	4
TAGGTCG	180	5.1686584E-8	13.361111	21
AGTACCG	195	1.0280928E-8	13.282052	5
TCTAACG	155	1.8904593E-6	13.129032	2
>>END_MODULE