FastQCFastQC Report
Wed 25 May 2016
SRR2088689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122942
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT247021.1635739459674357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT233711.1008779326048475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT171250.8066635828958116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94360.4444775222309418No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61360.2890328610013839No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59160.2786698835860801No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA53710.2529979622618046No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49430.2328372607447589No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG44920.211593157043386No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43480.2048101172806417No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28000.13189243983114No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA27660.13029088877604758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.13014957544765707No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25130.11837346474844815No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA24910.11733716700691775No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA24240.11418116933952978No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.10739812957678543No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA21580.10165138755557146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.025.9469091
GTATTGG5050.019.0495051
CGAATTA1258.589268E-917.7615
TATACCG953.6093006E-617.5263165
ATTGGAC6450.017.2093033
TGCGACG1005.88251E-616.65000222
GTATCAA100800.016.2609142
CCGAATT1455.3520125E-815.31034514
TACCCGC855.366811E-415.23529311
TTGGACC9550.014.7225124
GGACCCT8950.014.6759786
GTTACGC908.2780863E-414.38888822
TCACGTT1854.9039954E-914.024
ACAGCGT800.006300937413.8750018
TAGCGAA1203.3029486E-513.87499910
TATTGGA7950.013.7295592
GTATTAG4800.013.4895831
GACCCTC9350.013.4545457
CCCATAG2202.8558134E-1013.4545451
GATACTG6800.013.33088236
Produced by FastQC (version 0.11.5)