##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2122942
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.342994297536155	31.0	31.0	34.0	30.0	34.0
2	31.560100558564482	31.0	31.0	34.0	30.0	34.0
3	31.618360746548895	31.0	31.0	34.0	30.0	34.0
4	35.379849284624825	37.0	35.0	37.0	33.0	37.0
5	35.2412185542516	37.0	35.0	37.0	33.0	37.0
6	35.30267430763535	37.0	35.0	37.0	32.0	37.0
7	35.16282734054911	37.0	35.0	37.0	32.0	37.0
8	35.195505105650554	37.0	35.0	37.0	32.0	37.0
9	36.751075629951266	39.0	37.0	39.0	32.0	39.0
10	36.532121932676446	38.0	35.0	39.0	32.0	39.0
11	36.646456191455066	39.0	35.0	39.0	32.0	39.0
12	36.485684488789616	38.0	35.0	39.0	32.0	39.0
13	36.53730295033967	38.0	35.0	39.0	32.0	39.0
14	37.45428278304353	39.0	36.0	41.0	32.0	41.0
15	37.47119987262958	39.0	36.0	41.0	32.0	41.0
16	37.338897153101684	39.0	36.0	41.0	32.0	41.0
17	37.373932495565114	39.0	36.0	41.0	32.0	41.0
18	37.33995794515347	39.0	36.0	40.0	32.0	41.0
19	37.39374085585004	39.0	36.0	41.0	32.0	41.0
20	37.26418055698177	39.0	36.0	40.0	31.0	41.0
21	37.20564386591815	39.0	36.0	40.0	31.0	41.0
22	37.07019221438928	39.0	36.0	40.0	31.0	41.0
23	36.89151799719446	39.0	36.0	40.0	31.0	41.0
24	36.718424243337786	39.0	35.0	40.0	30.0	41.0
25	36.91680601730994	39.0	36.0	40.0	31.0	41.0
26	36.78008348791441	39.0	36.0	40.0	30.0	41.0
27	36.65102390927307	39.0	35.0	40.0	30.0	41.0
28	36.47684769532093	39.0	35.0	40.0	30.0	41.0
29	36.372630057721786	39.0	35.0	40.0	30.0	41.0
30	36.1639456000211	38.0	35.0	40.0	29.0	41.0
31	36.003797089133855	38.0	35.0	40.0	29.0	41.0
32	35.83401383551694	38.0	35.0	40.0	29.0	41.0
33	35.700459550943926	38.0	34.0	40.0	28.0	41.0
34	35.51461415337772	38.0	34.0	40.0	27.0	41.0
35	35.37845499311804	38.0	34.0	40.0	27.0	41.0
36	35.180686518991095	38.0	34.0	40.0	25.0	41.0
37	35.12267551350908	38.0	34.0	40.0	25.0	41.0
38	34.928320227307196	38.0	33.0	40.0	24.0	41.0
39	34.84425245720326	38.0	33.0	40.0	24.0	41.0
40	34.67242204450239	38.0	33.0	40.0	24.0	41.0
41	34.53382334515027	38.0	33.0	40.0	23.0	41.0
42	34.260901616718684	38.0	33.0	40.0	22.0	41.0
43	33.902360026793005	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	7.0
15	10.0
16	40.0
17	71.0
18	169.0
19	441.0
20	966.0
21	1951.0
22	3584.0
23	6175.0
24	10002.0
25	15157.0
26	21938.0
27	29910.0
28	39874.0
29	51966.0
30	64900.0
31	79467.0
32	97405.0
33	117035.0
34	142846.0
35	173057.0
36	221626.0
37	295820.0
38	397683.0
39	350840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.84503580408697	22.537780118345204	14.594086885086826	23.023097192481
2	19.342968390092615	24.042154707947745	34.301031304670595	22.313845597289045
3	20.051937358627793	24.89620536029717	32.72373903761855	22.328118243456487
4	14.348719842558111	17.193592665273002	35.844267059580524	32.613420432588356
5	12.418521090072174	39.04115138331617	35.02728760371221	13.513039922899447
6	32.25325986296376	37.71412502084372	13.656049011230643	16.37656610496189
7	25.634708814465963	33.406188204859106	21.77756151604707	19.18154146462786
8	26.730782093905532	35.05503212051954	19.109000622720735	19.10518516285419
9	24.441694591750505	14.50308110160334	21.893438445327288	39.16178586131886
10	16.10529161889491	28.328235062474622	33.948077714793904	21.618395603836564
11	32.98554553068336	22.66670497828014	23.554058471686933	20.793691019349563
12	20.514361673564327	27.58864820612151	31.35624995878361	20.540740161530554
13	31.28361490799089	21.291396561940928	26.392572194624254	21.032416335443926
14	20.15632080386558	23.24981087566217	28.112590923350712	28.48127739712154
15	22.4485171992452	30.066907150548627	25.558729348234664	21.92584630197151
16	21.478872244272335	28.715998835578173	27.00163263998734	22.803496280162154
17	21.35348021754716	27.50998378665079	27.481344285430314	23.65519171037174
18	22.53495385177739	26.129964926031896	29.709949683034203	21.625131539156513
19	22.33801017644382	25.323772387564052	31.153936376971203	21.184281059020922
20	23.20426087947763	25.034032959920715	30.789018258624118	20.972687901977537
21	22.14445802099162	25.797925708756996	31.288419561156168	20.76919670909521
22	20.940232940890517	26.14682831655316	30.928541618188344	21.984397124367977
23	21.91746171115367	26.3204552927023	31.532420574843776	20.22966242130025
24	21.805871286167967	26.337648414323144	29.472825917994932	22.383654381513956
25	21.808273612750607	26.53209555418848	30.481002307175608	21.178628525885305
26	21.43859794568104	27.314735871257906	30.304219333359082	20.94244684970197
27	20.540551743759366	27.182796326983972	30.188059777422087	22.088592151834575
28	21.067556249770366	26.75758452185693	30.345058885263942	21.82980034310876
29	21.221964613258393	26.569873317311544	29.813485248301646	22.394676821128414
30	21.44637017874252	26.849532394196356	31.026801485862542	20.67729594119858
31	22.02650849622835	26.727343469581367	30.78463754544401	20.461510488746278
32	21.64642274730068	26.533744209686372	30.034122458362027	21.78571058465092
33	21.263840462904778	26.535911014055024	30.584679185771446	21.615569337268752
34	20.623832398624174	26.978645671902484	31.07376461533099	21.323757314142355
35	20.26188186017329	28.645860320253686	30.1521661920109	20.94009162756213
36	22.24959513731416	26.598842549631595	29.94613135921754	21.205430953836704
37	21.392388487297346	27.150435574782545	30.057957306417226	21.399218631502887
38	21.146032251469894	25.928546328632624	31.21229878159648	21.713122638300998
39	20.692746198435945	25.757180365737735	31.883066047023423	21.667007388802897
40	19.85466395219464	26.18102614202366	31.445701295654803	22.51860861012689
41	21.01154906728493	25.480912808734296	31.398408435086782	22.109129688893997
42	20.020471590839506	27.150529783668137	30.71836159442886	22.110637031063497
43	20.300743025480678	27.025373279156945	30.124091944103988	22.549791751258393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	826.0
1	691.0
2	556.0
3	898.5
4	1241.0
5	1241.0
6	2145.0
7	3049.0
8	3432.0
9	3815.0
10	6003.5
11	8192.0
12	8192.0
13	15932.5
14	23673.0
15	37211.0
16	50749.0
17	47041.5
18	43334.0
19	43334.0
20	47666.5
21	51999.0
22	43034.0
23	34069.0
24	37613.5
25	41158.0
26	41158.0
27	45531.0
28	49904.0
29	54482.5
30	59061.0
31	64888.0
32	70715.0
33	70715.0
34	78752.0
35	86789.0
36	94285.5
37	101782.0
38	106939.5
39	112097.0
40	112097.0
41	115498.0
42	118899.0
43	124216.5
44	129534.0
45	136660.5
46	143787.0
47	143787.0
48	180523.0
49	217259.0
50	199871.5
51	182484.0
52	165564.0
53	148644.0
54	148644.0
55	132782.0
56	116920.0
57	101032.5
58	85145.0
59	76829.5
60	68514.0
61	68514.0
62	60369.5
63	52225.0
64	45445.0
65	38665.0
66	33010.0
67	27355.0
68	27355.0
69	23203.0
70	19051.0
71	15887.0
72	12723.0
73	10293.5
74	7864.0
75	7864.0
76	6386.0
77	4908.0
78	3859.5
79	2811.0
80	2171.0
81	1531.0
82	1531.0
83	1169.5
84	808.0
85	609.0
86	410.0
87	309.5
88	209.0
89	209.0
90	167.0
91	125.0
92	82.0
93	39.0
94	30.0
95	21.0
96	21.0
97	11.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2122942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.47228506581756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5520810273249	44.423282054310945
2	10.874553235603788	11.847235276264366
3	3.1828613588520307	5.201331937930897
4	1.3196244523603584	2.8753183739518735
5	0.710094892371286	1.9340245700514866
6	0.44564685777907925	1.4565241605376718
7	0.29278663769835706	1.1164130034517006
8	0.22592216170274057	0.9845197115965933
9	0.17581766213276695	0.8619470830171511
>10	1.0608099417077852	10.98507270617105
>50	0.098790259303	3.7121164748421362
>100	0.05269402747230756	5.220783196553304
>500	0.004332023797656418	1.649655674360917
>1k	0.0033789785621720007	3.387141331269912
>5k	3.465619038125129E-4	1.267611768543223
>10k+	2.599214278593847E-4	3.0770226771466196
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24702	1.1635739459674357	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23371	1.1008779326048475	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17125	0.8066635828958116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9436	0.4444775222309418	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6136	0.2890328610013839	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5916	0.2786698835860801	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5371	0.2529979622618046	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4943	0.2328372607447589	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4492	0.211593157043386	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4348	0.2048101172806417	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2800	0.13189243983114	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2766	0.13029088877604758	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2763	0.13014957544765707	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2513	0.11837346474844815	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2491	0.11733716700691775	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2424	0.11418116933952978	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2280	0.10739812957678543	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2158	0.10165138755557146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.710444279683571E-5	0.0
4	0.0	0.0	0.0	4.710444279683571E-5	0.0
5	4.710444279683571E-5	0.0	0.0	4.710444279683571E-5	0.0
6	9.420888559367142E-5	0.0	0.0	4.710444279683571E-5	0.0
7	9.420888559367142E-5	0.0	0.0	9.420888559367142E-5	0.0
8	9.420888559367142E-5	0.0	0.0	1.8841777118734285E-4	0.0
9	9.420888559367142E-5	0.0	0.0	2.8262665678101425E-4	0.0
10	2.3552221398417855E-4	0.0	0.0	3.768355423746857E-4	0.0
11	2.3552221398417855E-4	0.0	4.710444279683571E-5	4.710444279683571E-4	0.0
12	2.3552221398417855E-4	0.0	4.710444279683571E-5	7.536710847493714E-4	0.0
13	2.3552221398417855E-4	0.0	4.710444279683571E-5	8.949844131398786E-4	0.0
14	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.001130506627124057	0.0
15	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.0015073421694987428	0.0
16	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.0023081176970449497	0.0
17	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.0030146843389974856	0.0
18	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.0033444154385753357	0.0
19	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.0042393998517152144	0.0
20	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.0072540841907127	0.0
21	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.010692708514881707	0.0
22	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.01789968826279757	0.0
23	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.02185646145773177	0.0
24	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.027932934578523576	0.0
25	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.03174839444506727	0.0
26	2.8262665678101425E-4	0.0	9.420888559367142E-5	0.03966194083493567	0.0
27	3.2973109957784997E-4	0.0	9.420888559367142E-5	0.08464668370591377	0.0
28	3.2973109957784997E-4	0.0	9.420888559367142E-5	0.17471037833346365	0.0
29	3.2973109957784997E-4	0.0	9.420888559367142E-5	0.29021047207130485	0.0
30	3.2973109957784997E-4	0.0	9.420888559367142E-5	0.46840657917173434	0.0
31	3.2973109957784997E-4	0.0	9.420888559367142E-5	0.6916816380287356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6310	0.0	25.946909	1
GTATTGG	505	0.0	19.049505	1
CGAATTA	125	8.589268E-9	17.76	15
TATACCG	95	3.6093006E-6	17.526316	5
ATTGGAC	645	0.0	17.209303	3
TGCGACG	100	5.88251E-6	16.650002	22
GTATCAA	10080	0.0	16.260914	2
CCGAATT	145	5.3520125E-8	15.310345	14
TACCCGC	85	5.366811E-4	15.235293	11
TTGGACC	955	0.0	14.722512	4
GGACCCT	895	0.0	14.675978	6
GTTACGC	90	8.2780863E-4	14.388888	22
TCACGTT	185	4.9039954E-9	14.0	24
ACAGCGT	80	0.0063009374	13.875001	8
TAGCGAA	120	3.3029486E-5	13.874999	10
TATTGGA	795	0.0	13.729559	2
GTATTAG	480	0.0	13.489583	1
GACCCTC	935	0.0	13.454545	7
CCCATAG	220	2.8558134E-10	13.454545	1
GATACTG	680	0.0	13.330882	36
>>END_MODULE