FastQCFastQC Report
Wed 25 May 2016
SRR2088688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3946737
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT381550.9667479743393086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT356630.9036072076756064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT276380.7002746826048961No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146610.37147142056843413No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG96830.2453419115588396No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG84200.21334079265986056No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79220.20072277428164076No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA75410.19106923010071358No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG68180.17275029980462342No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66130.16755613561278596No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA46570.11799620800676609No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC46150.11693203778209696No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42000.1064170224669138No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41810.10593561212718253No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41290.10461806803949694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111650.023.0152261
GTATTGG12400.016.4112911
ATTGGAC13550.015.2915133
GGACCCT16500.014.86
GTATCAA173950.014.7510792
TTAACGG4900.014.34693835
CGCGAAT1304.4502776E-614.23076835
TTGGACC18150.014.1680454
GTATTAG10800.013.8749991
GACCCTC16700.013.8473057
TGCGGTA2151.9826984E-1013.76744236
TAACGGC5200.013.5192336
CGAACTA6200.013.4274224
TGGACCC18450.013.3360435
GGGTAAG5800.012.758621
TTACGCT1451.3738665E-512.758624
TAAACGT2756.184564E-1112.1090924
AGTACCG4300.012.0465125
TATTAGA10750.012.0465112
TATACTG6450.012.0465115
Produced by FastQC (version 0.11.5)