##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3946737
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60808080193841	31.0	31.0	34.0	30.0	34.0
2	31.811215188648244	31.0	31.0	34.0	30.0	34.0
3	31.89028303634116	31.0	31.0	34.0	30.0	34.0
4	35.60167525730749	37.0	35.0	37.0	33.0	37.0
5	35.47462524105356	37.0	35.0	37.0	33.0	37.0
6	35.520894095552855	37.0	35.0	37.0	33.0	37.0
7	35.391149701639605	37.0	35.0	37.0	33.0	37.0
8	35.41500738458124	37.0	35.0	37.0	33.0	37.0
9	37.02675982716862	39.0	37.0	39.0	33.0	39.0
10	36.83647377567849	39.0	37.0	39.0	32.0	39.0
11	36.9207525102382	39.0	37.0	39.0	33.0	39.0
12	36.785785320886596	39.0	37.0	39.0	32.0	39.0
13	36.82864274969424	39.0	37.0	39.0	32.0	39.0
14	37.84659276764578	40.0	37.0	41.0	32.0	41.0
15	37.85296233318815	40.0	37.0	41.0	32.0	41.0
16	37.738107454335065	40.0	37.0	41.0	32.0	41.0
17	37.748978713301646	39.0	37.0	41.0	32.0	41.0
18	37.705962672455755	39.0	37.0	41.0	32.0	41.0
19	37.74771564459451	40.0	37.0	41.0	32.0	41.0
20	37.623499614998416	39.0	37.0	41.0	32.0	41.0
21	37.56171921260525	39.0	36.0	41.0	32.0	41.0
22	37.435303137756584	39.0	36.0	41.0	32.0	41.0
23	37.2783002769123	39.0	36.0	41.0	31.0	41.0
24	37.12555815094849	39.0	36.0	41.0	31.0	41.0
25	37.31890850593794	39.0	36.0	41.0	31.0	41.0
26	37.21579699888794	39.0	36.0	41.0	31.0	41.0
27	37.11340127300096	39.0	36.0	41.0	31.0	41.0
28	36.960913027647905	39.0	36.0	41.0	31.0	41.0
29	36.86249121742847	39.0	35.0	41.0	30.0	41.0
30	36.67824306509402	39.0	35.0	40.0	30.0	41.0
31	36.54798254862181	39.0	35.0	40.0	30.0	41.0
32	36.38569962984612	38.0	35.0	40.0	30.0	41.0
33	36.267103685905596	38.0	35.0	40.0	30.0	41.0
34	36.10076627857392	38.0	35.0	40.0	29.0	41.0
35	35.963739413089854	38.0	35.0	40.0	29.0	41.0
36	35.780836169220294	38.0	35.0	40.0	27.0	41.0
37	35.741537122944855	38.0	35.0	40.0	27.0	41.0
38	35.56322729383792	38.0	35.0	40.0	26.0	41.0
39	35.490146670528084	38.0	34.0	40.0	26.0	41.0
40	35.34115574460624	38.0	34.0	40.0	25.0	41.0
41	35.224438567859984	38.0	34.0	40.0	25.0	41.0
42	34.99492669514082	38.0	34.0	40.0	24.0	41.0
43	34.65685223008273	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	3.0
14	9.0
15	25.0
16	51.0
17	105.0
18	290.0
19	618.0
20	1250.0
21	2612.0
22	4985.0
23	8741.0
24	14164.0
25	21923.0
26	32208.0
27	45342.0
28	61688.0
29	80448.0
30	103434.0
31	129141.0
32	158955.0
33	196782.0
34	242635.0
35	299818.0
36	386138.0
37	531869.0
38	740667.0
39	882831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.054699109669585	21.51932089723739	13.688320250373916	23.73765974271911
2	18.769429024533434	23.258580442527588	35.00339647663374	22.968594056305246
3	19.437626576080444	24.711400835677676	32.4200472440905	23.430925344151383
4	13.661538633053077	16.633183310669043	36.77992731717366	32.925350739104225
5	12.654428202335247	38.49169579832657	35.327233610955076	13.526642388383111
6	32.065602547116775	37.780221990976344	14.077755877830217	16.076419584076667
7	26.080101106306298	32.83621381409503	22.093871469013518	18.98981361058515
8	26.576384491796638	34.797758249409576	19.361589079789205	19.264268179004578
9	24.628243533835672	14.48908300705114	21.809332620846032	39.07334083826716
10	16.13155373666905	27.76957268751376	34.21527707572103	21.883596500096157
11	33.03592309292461	22.743750090264438	23.604055704750532	20.61627111206042
12	20.58736115429024	27.309015016708738	31.234967011989905	20.868656817011118
13	30.71570261712397	21.29047362416092	26.249684232823217	21.744139525891896
14	20.357297686671295	22.991853776930157	27.903176725482343	28.74767181091621
15	22.762018345787926	29.746268879836684	25.294287407547046	22.197425366828345
16	21.804239806199398	28.512819577286248	26.713738462937865	22.969202153576486
17	21.443942172989992	27.388422385378096	27.26900728373844	23.89862815789347
18	22.519590233653773	26.416530921619557	29.2924256164016	21.77145322832507
19	22.535071376684083	25.580371836278932	30.318159026051138	21.566397760985847
20	23.218344673080573	25.294287407547046	30.151996446684947	21.335371472687438
21	22.2305666681109	26.093301884569453	30.533704171319243	21.142427276000404
22	21.31358132046802	26.39116819793161	30.060705843840115	22.234544637760255
23	22.06698850214747	26.4403227273568	30.790169195464507	20.70251957503122
24	22.067089851692675	26.59085213937488	28.75175113011077	22.59030687882167
25	21.945166348809156	26.68862911311293	29.88932376289578	21.47688077518213
26	21.722906796171117	27.293229825042815	29.708820222882853	21.275043155903216
27	20.82411369189282	27.348794713202324	29.435531174233297	22.391560420671556
28	21.258320480944132	26.942357699537617	29.736057913157122	22.063263906361126
29	21.642308570345577	26.901843218841286	29.109768398553033	22.346079812260104
30	21.662097069047164	27.04786257609767	30.286107232379557	21.003933122475605
31	22.209739336570944	26.847950598177682	30.096760944547356	20.845549120704014
32	21.650391196575804	26.660631301249616	29.514735843812247	22.174241658362337
33	21.337297114046365	26.717615083042016	30.02376393461231	21.921323868299307
34	20.926071334370647	26.93622605205262	30.52488676088627	21.612815852690463
35	20.555106661528242	28.5028113096971	29.59026659237745	21.351815436397207
36	22.21916484427516	26.79476742433053	29.36136864452838	21.624699086865935
37	21.418782148392456	27.0621275245855	29.633973583747792	21.885116743274256
38	21.239418790763104	26.128571526301346	30.614074360668063	22.017935322267483
39	20.80024587399667	25.97963836962027	31.275557504845143	21.944558251537917
40	20.06414412716125	26.33129595410082	31.064370390020922	22.540189528717015
41	21.013966727451056	25.448313378874754	31.172180968734427	22.365538924939766
42	20.022388114536135	27.03397768840437	30.489870493017396	22.4537637040421
43	20.33462072593132	26.653891556493374	30.16099121882203	22.850496498753273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1075.0
1	1104.0
2	1133.0
3	1964.0
4	2795.0
5	2795.0
6	4354.0
7	5913.0
8	6450.5
9	6988.0
10	10578.0
11	14168.0
12	14168.0
13	26353.5
14	38539.0
15	59481.0
16	80423.0
17	75701.5
18	70980.0
19	70980.0
20	77803.5
21	84627.0
22	70968.0
23	57309.0
24	65333.5
25	73358.0
26	73358.0
27	82646.5
28	91935.0
29	100812.5
30	109690.0
31	122014.5
32	134339.0
33	134339.0
34	149943.0
35	165547.0
36	180683.5
37	195820.0
38	206917.5
39	218015.0
40	218015.0
41	224711.5
42	231408.0
43	242564.0
44	253720.0
45	262847.5
46	271975.0
47	271975.0
48	327287.0
49	382599.0
50	358339.0
51	334079.0
52	304251.5
53	274424.0
54	274424.0
55	248504.5
56	222585.0
57	192827.0
58	163069.0
59	147529.0
60	131989.0
61	131989.0
62	116587.0
63	101185.0
64	88149.0
65	75113.0
66	64156.0
67	53199.0
68	53199.0
69	45166.0
70	37133.0
71	30930.0
72	24727.0
73	20246.0
74	15765.0
75	15765.0
76	12587.0
77	9409.0
78	7405.0
79	5401.0
80	4242.5
81	3084.0
82	3084.0
83	2315.5
84	1547.0
85	1172.5
86	798.0
87	632.5
88	467.0
89	467.0
90	356.5
91	246.0
92	173.5
93	101.0
94	72.0
95	43.0
96	43.0
97	28.5
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3946737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.18423652712382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.35999237998892	37.275321705738826
2	12.469828914811849	12.016983717681239
3	4.341193828823784	6.275313307744064
4	1.9194002373060532	3.6993934009828986
5	0.9947461914808577	2.3965542887384625
6	0.6246024467645039	1.8057595218192672
7	0.38844211191377503	1.3101750618284185
8	0.27964014379042923	1.0779397464701566
9	0.19537701045117337	0.8472682875187513
>10	1.2036414403165736	11.042253030251016
>50	0.12618690468394395	4.240030395063528
>100	0.08556400929863307	7.893775874828838
>500	0.006851710418214877	2.2891453258612655
>1k	0.004005615305806338	3.687687933393699
>5k	3.162327873005004E-4	1.1935229207948796
>10k+	2.1082185820033359E-4	2.9488754812847424
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38155	0.9667479743393086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35663	0.9036072076756064	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27638	0.7002746826048961	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14661	0.37147142056843413	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9683	0.2453419115588396	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8420	0.21334079265986056	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7922	0.20072277428164076	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7541	0.19106923010071358	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6818	0.17275029980462342	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6613	0.16755613561278596	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4657	0.11799620800676609	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4615	0.11693203778209696	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4200	0.1064170224669138	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4181	0.10593561212718253	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4129	0.10461806803949694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.5337386301646143E-5	0.0
4	0.0	0.0	0.0	1.0134954520658457E-4	0.0
5	2.5337386301646143E-5	0.0	0.0	1.0134954520658457E-4	0.0
6	2.5337386301646143E-5	0.0	0.0	1.0134954520658457E-4	0.0
7	2.5337386301646143E-5	0.0	0.0	1.0134954520658457E-4	2.5337386301646143E-5
8	2.5337386301646143E-5	0.0	0.0	1.2668693150823073E-4	2.5337386301646143E-5
9	2.5337386301646143E-5	0.0	0.0	3.54723408223046E-4	2.5337386301646143E-5
10	2.5337386301646143E-5	0.0	0.0	4.053981808263383E-4	2.5337386301646143E-5
11	7.601215890493843E-5	0.0	0.0	4.3073556712798446E-4	2.5337386301646143E-5
12	7.601215890493843E-5	0.0	0.0	6.841094301444459E-4	2.5337386301646143E-5
13	7.601215890493843E-5	0.0	0.0	7.347842027477382E-4	2.5337386301646143E-5
14	7.601215890493843E-5	0.0	0.0	8.86808520557615E-4	2.5337386301646143E-5
15	7.601215890493843E-5	0.0	0.0	0.001064170224669138	2.5337386301646143E-5
16	7.601215890493843E-5	0.0	0.0	0.0015455805644004148	2.5337386301646143E-5
17	7.601215890493843E-5	0.0	0.0	0.002255027380846507	2.5337386301646143E-5
18	7.601215890493843E-5	0.0	0.0	0.0027111003342761373	2.5337386301646143E-5
19	1.5202431780987686E-4	0.0	0.0	0.003496559309627168	2.5337386301646143E-5
20	1.5202431780987686E-4	0.0	0.0	0.004966127715122644	2.5337386301646143E-5
21	1.5202431780987686E-4	0.0	0.0	0.008437349638448166	2.5337386301646143E-5
22	1.5202431780987686E-4	0.0	0.0	0.013631513830285626	2.5337386301646143E-5
23	1.5202431780987686E-4	0.0	0.0	0.017178747912516085	2.5337386301646143E-5
24	1.77361704111523E-4	0.0	0.0	0.02305702153449799	2.5337386301646143E-5
25	1.77361704111523E-4	0.0	0.0	0.028225848340033804	2.5337386301646143E-5
26	1.77361704111523E-4	0.0	0.0	0.03739798218122971	2.5337386301646143E-5
27	1.77361704111523E-4	0.0	0.0	0.09210139920648373	2.5337386301646143E-5
28	1.77361704111523E-4	0.0	0.0	0.20092547337205394	2.5337386301646143E-5
29	1.77361704111523E-4	0.0	0.0	0.3328572438447254	2.5337386301646143E-5
30	2.0269909041316914E-4	0.0	0.0	0.5210380119070513	2.5337386301646143E-5
31	2.0269909041316914E-4	0.0	0.0	0.7594628170055415	7.601215890493843E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11165	0.0	23.015226	1
GTATTGG	1240	0.0	16.411291	1
ATTGGAC	1355	0.0	15.291513	3
GGACCCT	1650	0.0	14.8	6
GTATCAA	17395	0.0	14.751079	2
TTAACGG	490	0.0	14.346938	35
CGCGAAT	130	4.4502776E-6	14.230768	35
TTGGACC	1815	0.0	14.168045	4
GTATTAG	1080	0.0	13.874999	1
GACCCTC	1670	0.0	13.847305	7
TGCGGTA	215	1.9826984E-10	13.767442	36
TAACGGC	520	0.0	13.51923	36
CGAACTA	620	0.0	13.42742	24
TGGACCC	1845	0.0	13.336043	5
GGGTAAG	580	0.0	12.75862	1
TTACGCT	145	1.3738665E-5	12.75862	4
TAAACGT	275	6.184564E-11	12.109092	4
AGTACCG	430	0.0	12.046512	5
TATTAGA	1075	0.0	12.046511	2
TATACTG	645	0.0	12.046511	5
>>END_MODULE