FastQCFastQC Report
Wed 25 May 2016
SRR2088687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466494
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT177681.2115971834866015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162321.1068575800514697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119090.8120728758522026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67420.4597359416404022No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG37820.2578939975206172No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG37390.2549618341432014No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA34540.23552772803707347No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32010.21827569700251076No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28200.19229536568168706No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG27850.18990872107216258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.13201554182969724No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA18760.1279241510705124No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC18570.12662854399677054No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.12274172277554495No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA16190.11039936065200404No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15740.1073308175826154No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.10494417297309093No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14950.10194381974968872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG451.3234555E-424.66666623
CGATACG400.001931439523.12500226
GTATTGG4650.022.677421
GGTATCA50800.021.9596461
TGGACCC6900.018.55
GGACCCT7250.017.8620686
ATACGGG953.6077618E-617.5263143
TATACGG953.6077618E-617.5263142
CGTTTAC650.001580215217.07692328
GTATACG1107.8112134E-716.8181821
ATTGGAC6200.016.4112913
GACCCTC7700.016.0974037
TTGGACC8100.015.9876554
CTAATAC3250.015.9384613
CGAACTA2250.015.62222224
TTAACGG1557.212293E-915.516129535
GGCGTCG855.3654023E-415.2352948
GACCGTC750.00410512314.822
CGGGCGT1001.0936903E-414.86
CTTAGAC1252.9599087E-614.7999993
Produced by FastQC (version 0.11.5)