FastQCFastQC Report
Wed 25 May 2016
SRR2088686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845539
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT359861.264646170725476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324151.1391514929157533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT254110.8930118336104338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138690.4873944795696No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77980.27404298447499753No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG73460.2581584719098912No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA68480.24065739390674315No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA65720.2309580012784924No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG60180.211488930568163No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55100.1936364252958754No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40510.14236318672841947No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC38320.13466692953426398No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37900.133190935003878No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA36070.1267598159786248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.12584610507886201No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34980.12292925874500403No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32910.11565471427381596No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30320.1065527480031024No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG29240.10275733349639558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106850.022.5428181
GTATTGG9300.019.29571
TTGGACC12950.017.1428574
ATTGGAC9550.016.8534033
ACGTTTA2003.6379788E-1215.72526
GGACCCT13850.015.4945856
GTATCAA159250.015.1020412
GCGTAAG750.004106384714.81
TATTGGA12600.014.682542
TGGACCC15600.014.5865375
GTATTAG3450.014.478261
TAGTACT4400.013.8754
GAACGGA1356.5746553E-613.7037046
GACCCTC15100.013.3543057
TATACCG1551.8901101E-613.1290325
TATTCCG850.0094091913.0588245
GTACGTC850.0094091913.0588249
TTAATAC6500.012.8076933
ACCCTCG15300.012.4542488
TAAACGT2102.8958311E-812.3333344
Produced by FastQC (version 0.11.5)