##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2845539
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59021225855629	31.0	31.0	34.0	30.0	34.0
2	31.79029491424999	31.0	31.0	34.0	30.0	34.0
3	31.86055330817817	31.0	31.0	34.0	30.0	34.0
4	35.57582904328495	37.0	35.0	37.0	33.0	37.0
5	35.45676478164594	37.0	35.0	37.0	33.0	37.0
6	35.50530215892314	37.0	35.0	37.0	33.0	37.0
7	35.36663176993884	37.0	35.0	37.0	33.0	37.0
8	35.402197615284834	37.0	35.0	37.0	33.0	37.0
9	36.99633391072834	39.0	37.0	39.0	33.0	39.0
10	36.820499385177996	39.0	37.0	39.0	32.0	39.0
11	36.91303088799697	39.0	37.0	39.0	33.0	39.0
12	36.767155888568034	39.0	37.0	39.0	32.0	39.0
13	36.813203403643385	39.0	37.0	39.0	32.0	39.0
14	37.790139934824296	40.0	37.0	41.0	32.0	41.0
15	37.81089909503964	40.0	37.0	41.0	32.0	41.0
16	37.69677590080473	39.0	37.0	41.0	32.0	41.0
17	37.7345603065008	39.0	37.0	41.0	32.0	41.0
18	37.69805896176436	39.0	37.0	41.0	32.0	41.0
19	37.74444736129078	40.0	37.0	41.0	32.0	41.0
20	37.63370349167592	39.0	37.0	41.0	32.0	41.0
21	37.57328857555633	39.0	37.0	41.0	32.0	41.0
22	37.445506106224514	39.0	36.0	41.0	32.0	41.0
23	37.264778658805945	39.0	36.0	41.0	31.0	41.0
24	37.11054425892599	39.0	36.0	41.0	31.0	41.0
25	37.290605048815	39.0	36.0	41.0	31.0	41.0
26	37.16334444897786	39.0	36.0	41.0	31.0	41.0
27	37.050117394279255	39.0	36.0	41.0	31.0	41.0
28	36.87427197448357	39.0	35.0	41.0	30.0	41.0
29	36.75640502555052	39.0	35.0	41.0	30.0	41.0
30	36.55369474816546	39.0	35.0	40.0	30.0	41.0
31	36.38439993266653	38.0	35.0	40.0	30.0	41.0
32	36.20990469643888	38.0	35.0	40.0	30.0	41.0
33	36.05921514342274	38.0	35.0	40.0	30.0	41.0
34	35.8682372654179	38.0	35.0	40.0	29.0	41.0
35	35.73029257374438	38.0	35.0	40.0	28.0	41.0
36	35.52422370594815	38.0	34.0	40.0	27.0	41.0
37	35.44495366255743	38.0	34.0	40.0	26.0	41.0
38	35.234302886026164	38.0	34.0	40.0	25.0	41.0
39	35.12765384695132	38.0	34.0	40.0	25.0	41.0
40	34.94960392389632	38.0	34.0	40.0	24.0	41.0
41	34.80564244594785	38.0	34.0	40.0	23.0	41.0
42	34.5269356701841	38.0	33.0	40.0	22.0	41.0
43	34.161720854994435	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	3.0
15	21.0
16	31.0
17	76.0
18	185.0
19	458.0
20	1079.0
21	2177.0
22	4081.0
23	7156.0
24	11220.0
25	17228.0
26	25089.0
27	35148.0
28	47459.0
29	61782.0
30	77567.0
31	95772.0
32	118326.0
33	145555.0
34	178660.0
35	219661.0
36	282264.0
37	389317.0
38	517854.0
39	607361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17399199237825	21.500882609586444	14.186486286077962	23.13863911195735
2	18.800761472606773	23.716244971515064	34.858211396856625	22.624782159021542
3	19.944762661836652	24.33556524791964	33.0299813146121	22.68969077563161
4	13.90544989894709	16.52790560944693	35.81451528163909	33.752129209966895
5	12.723389136469402	38.57726075797942	34.72495017639892	13.974399929152263
6	32.407287336423785	37.00708371946405	13.684894144835127	16.900734799277046
7	25.577403788877962	33.41405617705468	21.559360107171262	19.4491799268961
8	27.001141084342894	34.46668627630828	19.161185279836264	19.370987359512558
9	24.480072140989808	14.508428807336676	22.045735447660356	38.96576360401316
10	16.250699779549674	28.19153067309919	33.542291987563694	22.015477559787442
11	32.86712991809285	22.709546416337993	23.488063245662772	20.93526041990639
12	20.741834850971998	27.39372048669865	31.282966074265715	20.581478588063632
13	31.553248786960925	21.184352068272478	26.307283084153827	20.95511606061277
14	20.222355061729957	23.46023020594692	28.235599652649285	28.081815079673834
15	22.574176632265452	29.87458615046218	25.601371128633275	21.949866088639094
16	21.418016059523346	28.61942851600347	27.067912265479404	22.89464315899378
17	21.306929899748344	27.231923371986817	27.478871314011162	23.982275414253678
18	22.78875812280204	25.815987761896782	29.75534687804314	21.639907237258036
19	22.3370335110501	25.324270726916765	31.181895591661192	21.15680017037194
20	23.39289674117979	24.93028561548445	30.67074462869776	21.006073014637998
21	22.203034293327203	25.71207071841222	31.405789904829984	20.679105083430592
22	20.939969545312856	26.099167855369405	30.900999775437977	22.059862823879765
23	21.893954010118996	26.19595092529043	31.509742091041453	20.400352973549126
24	21.74466770618853	26.163654759256506	29.82984945910072	22.261828075454247
25	21.655194323465608	26.537819372709354	30.600142890327632	21.20684341349741
26	21.349382313860396	27.181458416138383	30.478478769751533	20.990680500249688
27	20.416975483379424	27.256698994461154	30.43644806836244	21.889877453796977
28	21.023292950825837	26.861167603044628	30.19533381900582	21.920205627123718
29	20.922538752763536	26.781428755676867	29.94093561887572	22.355096872683873
30	21.25122867758973	27.128674040313626	31.203894938709325	20.416202343387315
31	21.889912596523892	26.88404551826561	30.85362737955797	20.372414505652532
32	21.543299880971585	26.778828193885236	29.99719912466496	21.680672800478224
33	21.147627918647398	26.665528042314655	30.548693938125602	21.63815010091234
34	20.323003831611516	27.136756867503838	31.190892129751163	21.349347171133484
35	20.01757839200236	28.755852581883435	30.25922329653538	20.967345729578827
36	21.872446661247658	26.805782665428236	30.194349822652228	21.12742085067188
37	21.1866714882488	27.433361482657592	30.079187106555207	21.3007799225384
38	20.87667749414083	26.24255018117833	31.241778798322567	21.638993526358274
39	20.48578494267694	25.88212637394884	32.020998482185625	21.6110902011886
40	19.496622608229934	26.23197222037723	31.73753724689769	22.533867924495148
41	20.722892921165375	25.565736403542527	31.667814076700406	22.04355659859169
42	19.60640848710912	27.195093794181	31.0741128482161	22.12438487049378
43	19.79125923067651	27.206269181339632	30.385631685244867	22.616839902738988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1202.0
1	1027.0
2	852.0
3	1566.0
4	2280.0
5	2280.0
6	3670.0
7	5060.0
8	5581.0
9	6102.0
10	9624.0
11	13146.0
12	13146.0
13	24677.5
14	36209.0
15	55752.5
16	75296.0
17	69882.5
18	64469.0
19	64469.0
20	69366.5
21	74264.0
22	61012.5
23	47761.0
24	53664.0
25	59567.0
26	59567.0
27	66075.0
28	72583.0
29	77043.0
30	81503.0
31	87244.0
32	92985.0
33	92985.0
34	100643.0
35	108301.0
36	116471.5
37	124642.0
38	130977.5
39	137313.0
40	137313.0
41	140372.0
42	143431.0
43	149794.0
44	156157.0
45	168912.5
46	181668.0
47	181668.0
48	233344.0
49	285020.0
50	264703.5
51	244387.0
52	223184.0
53	201981.0
54	201981.0
55	180764.5
56	159548.0
57	140039.0
58	120530.0
59	109132.5
60	97735.0
61	97735.0
62	86811.0
63	75887.0
64	66282.0
65	56677.0
66	48828.0
67	40979.0
68	40979.0
69	34820.5
70	28662.0
71	24232.5
72	19803.0
73	16187.0
74	12571.0
75	12571.0
76	10180.0
77	7789.0
78	6025.0
79	4261.0
80	3347.5
81	2434.0
82	2434.0
83	1834.5
84	1235.0
85	943.0
86	651.0
87	490.5
88	330.0
89	330.0
90	240.0
91	150.0
92	108.5
93	67.0
94	49.5
95	32.0
96	32.0
97	22.5
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2845539.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.19419118190327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82158322834071	41.140287842803154
2	12.301684974841127	12.841529948728095
3	3.975618664015914	6.225126018479683
4	1.6476840216539081	3.439981393342853
5	0.8593950327248013	2.242771431940815
6	0.49817958883056745	1.560124842140676
7	0.3214183288492897	1.1743318793729378
8	0.2263783458203271	0.9452507728951333
9	0.17113220584018216	0.8038896362102961
>10	1.0124390181678193	10.04491496775617
>50	0.0994516788714796	3.6212371528870015
>100	0.05653203249500911	5.54965373578972
>500	0.003914023919337686	1.3926895561359807
>1k	0.00391402391931511	3.8133065782710056
>5k	4.0489902613604587E-4	1.4121322631349402
>10k+	2.6993268409069725E-4	3.7927719801115805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35986	1.264646170725476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32415	1.1391514929157533	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25411	0.8930118336104338	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13869	0.4873944795696	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7798	0.27404298447499753	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7346	0.2581584719098912	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6848	0.24065739390674315	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6572	0.2309580012784924	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6018	0.211488930568163	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5510	0.1936364252958754	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4051	0.14236318672841947	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3832	0.13466692953426398	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3790	0.133190935003878	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3607	0.1267598159786248	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3581	0.12584610507886201	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3498	0.12292925874500403	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3291	0.11565471427381596	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3032	0.1065527480031024	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2924	0.10275733349639558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.514272691395198E-5	0.0	0.0	0.0	0.0
7	3.514272691395198E-5	0.0	0.0	0.0	0.0
8	7.028545382790396E-5	0.0	0.0	0.0	0.0
9	7.028545382790396E-5	0.0	0.0	7.028545382790396E-5	0.0
10	7.028545382790396E-5	0.0	0.0	1.4057090765580793E-4	0.0
11	1.757136345697599E-4	0.0	0.0	2.8114181531161585E-4	0.0
12	1.757136345697599E-4	0.0	0.0	3.514272691395198E-4	0.0
13	1.757136345697599E-4	0.0	0.0	4.2171272296742375E-4	0.0
14	1.757136345697599E-4	0.0	0.0	6.677118113650876E-4	0.0
15	1.757136345697599E-4	0.0	0.0	9.137108997627515E-4	0.0
16	1.757136345697599E-4	0.0	0.0	0.0015111372572999351	0.0
17	1.757136345697599E-4	0.0	0.0	0.0022842772494068788	0.0
18	2.4599908839766386E-4	0.0	0.0	0.0027059899723743024	0.0
19	2.4599908839766386E-4	0.0	0.0	0.003408844510653342	0.0
20	2.4599908839766386E-4	0.0	0.0	0.005903978121543933	0.0
21	2.8114181531161585E-4	0.0	0.0	0.010226533531960026	0.0
22	3.162845422255678E-4	0.0	0.0	0.017501078003148086	0.0
23	3.162845422255678E-4	0.0	0.0	0.02164791977899442	0.0
24	3.162845422255678E-4	0.0	0.0	0.02807903880424763	0.0
25	3.162845422255678E-4	0.0	0.0	0.03320987693368462	3.514272691395198E-5
26	3.162845422255678E-4	0.0	0.0	0.04406897955009578	3.514272691395198E-5
27	3.162845422255678E-4	0.0	0.0	0.1047956116574048	3.514272691395198E-5
28	3.162845422255678E-4	0.0	0.0	0.2209423241080161	3.514272691395198E-5
29	3.162845422255678E-4	0.0	0.0	0.3803848761166162	3.514272691395198E-5
30	3.162845422255678E-4	0.0	0.0	0.5972857866295278	3.514272691395198E-5
31	3.162845422255678E-4	0.0	0.0	0.8605399539419422	3.514272691395198E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10685	0.0	22.542818	1
GTATTGG	930	0.0	19.2957	1
TTGGACC	1295	0.0	17.142857	4
ATTGGAC	955	0.0	16.853403	3
ACGTTTA	200	3.6379788E-12	15.725	26
GGACCCT	1385	0.0	15.494585	6
GTATCAA	15925	0.0	15.102041	2
GCGTAAG	75	0.0041063847	14.8	1
TATTGGA	1260	0.0	14.68254	2
TGGACCC	1560	0.0	14.586537	5
GTATTAG	345	0.0	14.47826	1
TAGTACT	440	0.0	13.875	4
GAACGGA	135	6.5746553E-6	13.703704	6
GACCCTC	1510	0.0	13.354305	7
TATACCG	155	1.8901101E-6	13.129032	5
TATTCCG	85	0.00940919	13.058824	5
GTACGTC	85	0.00940919	13.058824	9
TTAATAC	650	0.0	12.807693	3
ACCCTCG	1530	0.0	12.454248	8
TAAACGT	210	2.8958311E-8	12.333334	4
>>END_MODULE