FastQCFastQC Report
Wed 25 May 2016
SRR2088685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823243
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT387371.3720745964835475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT373191.3218486683576298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287451.0181553624679136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153210.5426737974733312No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG100060.3544151176501633No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG92540.3277790824240067No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA86520.30645608613923775No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA82970.2938818939779537No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA74840.26508522291563286No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74150.26264122500259457No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA46210.16367701965434786No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA45680.16179974589505755No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44810.15871818330905277No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC44320.15698259058819947No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.15251963787743386No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG39550.14008712675458684No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.13948498234122958No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38960.13799733143764104No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38570.13661594131288027No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC33850.1198975787773139No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31600.11192802036523247No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG31540.11171549880757696No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG31270.11075915179812718No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30530.10813805258704262No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28430.10069979806909997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125550.022.0732771
GATCGGT603.7287617E-521.58333411
GTATTGG10750.020.3069761
ATTGGAC11650.018.1030043
TTAACGG3450.017.1594235
TTGGACC17200.015.8110474
GGACCCT16750.015.3522396
TAACGGC3900.015.17948736
GTATTAG5850.014.8632481
ATTCGCG750.004106375414.829
GTATCAA188600.014.7529162
CGAACTA4650.014.72043124
TGGACCC17450.014.6303735
TATTGGA13950.014.5878142
TATACCG908.279276E-414.3888895
TAATACT6650.014.187974
ATCCGTC1458.922434E-714.0344837
CTAATAC6100.013.9508193
TCTAATA6350.013.6929132
TCGGTTC950.001245859913.63157913
Produced by FastQC (version 0.11.5)