##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2823243
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.581855688653086	31.0	31.0	34.0	30.0	34.0
2	31.771878651607388	31.0	31.0	34.0	30.0	34.0
3	31.835472539912434	31.0	31.0	34.0	30.0	34.0
4	35.54522724398856	37.0	35.0	37.0	33.0	37.0
5	35.44150290995143	37.0	35.0	37.0	33.0	37.0
6	35.50340052202379	37.0	35.0	37.0	33.0	37.0
7	35.35590453956673	37.0	35.0	37.0	33.0	37.0
8	35.401925728674435	37.0	35.0	37.0	33.0	37.0
9	36.9896030911969	39.0	37.0	39.0	33.0	39.0
10	36.824493322041356	39.0	37.0	39.0	32.0	39.0
11	36.91382994662521	39.0	37.0	39.0	33.0	39.0
12	36.769379752291954	39.0	37.0	39.0	32.0	39.0
13	36.82054715091829	39.0	37.0	39.0	32.0	39.0
14	37.77693064323546	40.0	37.0	41.0	32.0	41.0
15	37.793884196294826	40.0	37.0	41.0	32.0	41.0
16	37.66881773903274	39.0	37.0	41.0	32.0	41.0
17	37.73033281230131	39.0	37.0	41.0	32.0	41.0
18	37.702739013255325	39.0	37.0	41.0	32.0	41.0
19	37.75800276490546	40.0	37.0	41.0	32.0	41.0
20	37.642865669019635	39.0	37.0	41.0	32.0	41.0
21	37.58827915273322	39.0	37.0	41.0	32.0	41.0
22	37.45832611645544	39.0	36.0	41.0	32.0	41.0
23	37.27902805390822	39.0	36.0	41.0	31.0	41.0
24	37.12254665999349	39.0	36.0	41.0	31.0	41.0
25	37.29188596234897	39.0	36.0	41.0	31.0	41.0
26	37.15820388113953	39.0	36.0	41.0	31.0	41.0
27	37.044625985081694	39.0	36.0	41.0	31.0	41.0
28	36.86470629697833	39.0	35.0	41.0	30.0	41.0
29	36.74503611626771	39.0	35.0	41.0	30.0	41.0
30	36.51778823147706	39.0	35.0	40.0	30.0	41.0
31	36.330285419993956	38.0	35.0	40.0	30.0	41.0
32	36.15742994846706	38.0	35.0	40.0	30.0	41.0
33	36.002075273010504	38.0	35.0	40.0	30.0	41.0
34	35.805073456305394	38.0	35.0	40.0	29.0	41.0
35	35.64274984477071	38.0	35.0	40.0	27.0	41.0
36	35.43319473385748	38.0	34.0	40.0	26.0	41.0
37	35.34078965218367	38.0	34.0	40.0	26.0	41.0
38	35.13061008209353	38.0	34.0	40.0	25.0	41.0
39	35.016224604116616	38.0	34.0	40.0	24.0	41.0
40	34.81099926573802	38.0	34.0	40.0	23.0	41.0
41	34.64893103427512	38.0	33.0	40.0	23.0	41.0
42	34.346964820243954	38.0	33.0	40.0	21.0	41.0
43	33.97875811610974	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	10.0
14	7.0
15	19.0
16	41.0
17	88.0
18	176.0
19	501.0
20	1009.0
21	2069.0
22	3862.0
23	6764.0
24	11244.0
25	17338.0
26	25532.0
27	35779.0
28	48117.0
29	62564.0
30	77985.0
31	96660.0
32	119578.0
33	146282.0
34	178820.0
35	217869.0
36	280597.0
37	389204.0
38	512678.0
39	588446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38180808382417	21.442539660950192	14.412397374225314	22.763254881000325
2	18.810885212502075	23.504069610727804	35.32813151400712	22.356913662763
3	19.9703674108109	24.58222689297379	32.97466778453006	22.47273791168525
4	13.688371847552618	16.347476997197905	36.01060907615816	33.953542079091314
5	12.38026623992338	38.73534796685939	35.00839283051441	13.87599296270282
6	32.10637554046888	36.941311817650835	13.317380048405328	17.63493259347495
7	25.450589977554184	33.452416246139634	21.549402584191302	19.547591192114883
8	26.820433097682344	34.741749116175974	18.939248233326	19.49856955281568
9	24.032469043578608	14.66742324341192	22.320324534586643	38.97978317842283
10	16.49840272339292	27.720816096949502	33.561475225476514	22.21930595418106
11	32.29456337977283	22.46522881664809	23.847929491014412	21.392278312564663
12	20.8563697846767	27.69843049287645	31.143015319616485	20.302184402830363
13	31.64431825386621	20.972016932300903	26.841756093967117	20.54190871986577
14	20.024879190349537	23.52471962208	28.440909974805567	28.009491212764893
15	22.38425810318134	29.594193627682774	26.363759690540277	21.657788578595607
16	21.084405416041058	28.600265722787586	27.461185594013692	22.85414326715766
17	21.134666764426584	26.926800137288925	27.617318098371268	24.32121499991322
18	22.703465482779908	25.45778029025486	30.323036309662328	21.515717917302904
19	22.27976833733405	24.65678653945126	31.80749938988603	21.25594573332866
20	23.43099761515392	24.14202390655002	31.60840211062243	20.81857636767363
21	21.89683991069844	25.374365578875075	32.39090648591	20.337888024516488
22	20.370970546991526	25.645720187741546	31.81327289220234	22.17003637306459
23	21.611104676430614	25.87226816820231	32.81584334044218	19.700783814924893
24	21.625768663908847	25.877510366624477	30.193433579752078	22.303287389714594
25	21.48989654804776	26.268337511152957	31.241058598214888	21.000707342584395
26	21.171999718054735	27.030439816905595	31.28728203700496	20.510278428034713
27	20.00621271353546	26.91298623604132	30.998111037555038	22.08269001286818
28	20.526146704339652	26.503988498333296	31.192674523588654	21.77719027373839
29	20.978073796694087	26.254169407309252	30.43032427601875	22.337432519977913
30	21.086920254473313	26.53802736781779	32.148490229144286	20.22656214856461
31	21.794864983283407	26.255161174578312	31.844548981437303	20.105424860700975
32	21.277693772728735	26.07338440226364	30.841128446966838	21.807793378040785
33	20.72729835866059	26.13629078332967	31.579747120598544	21.556663737411196
34	20.04092456795253	26.686013212465237	32.12482949572531	21.14823272385693
35	19.604051085932028	28.850439016407726	30.763380977124534	20.782128920535712
36	21.954645774380737	26.399888355341712	30.64815887261564	20.99730699766191
37	20.98165124291462	27.025303879262253	30.680603830417713	21.31244104740541
38	20.700201860059515	25.547428967325875	32.12369604741781	21.628673125196805
39	20.286564068342685	25.276676502872757	32.777447779025756	21.659311649758806
40	19.071259540889677	26.067823421504986	32.29013584732168	22.570781190283657
41	20.746106516513102	25.06659894313029	32.19513871104967	21.992155829306938
42	19.30850443975244	27.225605447352564	31.511633961369956	21.954256151525037
43	19.783313019814447	27.121505304360976	30.508673890274412	22.586507785550165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1236.0
1	1038.0
2	840.0
3	1629.0
4	2418.0
5	2418.0
6	4070.5
7	5723.0
8	6199.5
9	6676.0
10	10220.5
11	13765.0
12	13765.0
13	26085.5
14	38406.0
15	59358.0
16	80310.0
17	74029.5
18	67749.0
19	67749.0
20	72638.0
21	77527.0
22	60803.0
23	44079.0
24	48147.0
25	52215.0
26	52215.0
27	56745.5
28	61276.0
29	65993.5
30	70711.0
31	77341.5
32	83972.0
33	83972.0
34	92831.5
35	101691.0
36	111470.0
37	121249.0
38	128308.5
39	135368.0
40	135368.0
41	140067.5
42	144767.0
43	154264.0
44	163761.0
45	177262.0
46	190763.0
47	190763.0
48	254183.5
49	317604.0
50	292368.5
51	267133.0
52	240018.0
53	212903.0
54	212903.0
55	187790.5
56	162678.0
57	135320.5
58	107963.0
59	95794.0
60	83625.0
61	83625.0
62	73939.5
63	64254.0
64	55811.5
65	47369.0
66	40403.0
67	33437.0
68	33437.0
69	28511.0
70	23585.0
71	19601.0
72	15617.0
73	12821.5
74	10026.0
75	10026.0
76	7888.0
77	5750.0
78	4475.0
79	3200.0
80	2507.5
81	1815.0
82	1815.0
83	1349.0
84	883.0
85	666.5
86	450.0
87	335.0
88	220.0
89	220.0
90	182.0
91	144.0
92	101.5
93	59.0
94	36.0
95	13.0
96	13.0
97	11.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2823243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.00254732772116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69548874955441	38.25586470506355
2	11.671628393715231	11.205357887217792
3	3.6310951035316132	5.229054436761985
4	1.541247565920507	2.959352369073367
5	0.8131907691651874	1.9517614191658939
6	0.47981742130160365	1.3819475084817214
7	0.33964174328594043	1.1412568199587152
8	0.2351267615069224	0.9029346797799879
9	0.17579363980924484	0.7594688263370079
>10	1.1998216255804068	11.12402775970921
>50	0.1244808676814491	4.115986596478365
>100	0.07992815585344906	7.507906511165798
>500	0.006591796949886916	2.0908452648347415
>1k	0.005406754801588855	5.28646914789636
>5k	3.7032567134170235E-4	1.461305617765826
>10k+	3.7032567134170235E-4	4.626460450309752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38737	1.3720745964835475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37319	1.3218486683576298	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28745	1.0181553624679136	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15321	0.5426737974733312	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10006	0.3544151176501633	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9254	0.3277790824240067	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8652	0.30645608613923775	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8297	0.2938818939779537	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7484	0.26508522291563286	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7415	0.26264122500259457	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4621	0.16367701965434786	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4568	0.16179974589505755	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4481	0.15871818330905277	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4432	0.15698259058819947	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4306	0.15251963787743386	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3955	0.14008712675458684	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.13948498234122958	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3896	0.13799733143764104	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3857	0.13661594131288027	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3385	0.1198975787773139	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3160	0.11192802036523247	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3154	0.11171549880757696	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3127	0.11075915179812718	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3053	0.10813805258704262	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2843	0.10069979806909997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.084051921850156E-5
2	0.0	0.0	0.0	0.0	7.084051921850156E-5
3	0.0	0.0	0.0	0.0	1.0626077882775235E-4
4	0.0	0.0	0.0	3.542025960925078E-5	1.0626077882775235E-4
5	0.0	0.0	0.0	3.542025960925078E-5	1.0626077882775235E-4
6	0.0	0.0	0.0	3.542025960925078E-5	1.0626077882775235E-4
7	0.0	0.0	0.0	7.084051921850156E-5	1.0626077882775235E-4
8	7.084051921850156E-5	0.0	0.0	1.0626077882775235E-4	1.0626077882775235E-4
9	1.4168103843700312E-4	0.0	0.0	4.6046337492026013E-4	1.0626077882775235E-4
10	2.4794181726475544E-4	0.0	0.0	7.438254517942664E-4	1.0626077882775235E-4
11	2.4794181726475544E-4	0.0	0.0	0.0012397090863237772	1.0626077882775235E-4
12	2.4794181726475544E-4	0.0	0.0	0.0020543750573365454	1.0626077882775235E-4
13	2.4794181726475544E-4	0.0	0.0	0.0023377371342105514	1.0626077882775235E-4
14	2.8336207687400624E-4	0.0	0.0	0.002621099211084558	1.0626077882775235E-4
15	2.8336207687400624E-4	0.0	0.0	0.0037545475185805826	1.0626077882775235E-4
16	2.8336207687400624E-4	0.0	0.0	0.0053484592009968674	1.0626077882775235E-4
17	2.8336207687400624E-4	0.0	0.0	0.006906950623803902	1.0626077882775235E-4
18	2.8336207687400624E-4	0.0	0.0	0.008111239450518429	1.0626077882775235E-4
19	2.8336207687400624E-4	0.0	0.0	0.010236455027073476	1.0626077882775235E-4
20	2.8336207687400624E-4	0.0	0.0	0.016222478901036858	1.0626077882775235E-4
21	2.8336207687400624E-4	0.0	0.0	0.025679688216706815	1.4168103843700312E-4
22	2.8336207687400624E-4	0.0	0.0	0.041547964521651166	1.4168103843700312E-4
23	2.8336207687400624E-4	0.0	0.0	0.05121769539497663	1.4168103843700312E-4
24	2.8336207687400624E-4	0.0	0.0	0.06566916131555095	1.4168103843700312E-4
25	2.8336207687400624E-4	0.0	0.0	0.07473674777551914	1.4168103843700312E-4
26	2.8336207687400624E-4	0.0	0.0	0.09180931290717802	1.4168103843700312E-4
27	2.8336207687400624E-4	0.0	0.0	0.15460943319437964	1.4168103843700312E-4
28	3.1878233648325704E-4	0.0	0.0	0.295617486698807	1.4168103843700312E-4
29	3.1878233648325704E-4	0.0	0.0	0.4725062631874054	1.4168103843700312E-4
30	3.1878233648325704E-4	0.0	0.0	0.7345099235170335	1.4168103843700312E-4
31	3.1878233648325704E-4	0.0	0.0	1.070896129026088	1.4168103843700312E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12555	0.0	22.073277	1
GATCGGT	60	3.7287617E-5	21.583334	11
GTATTGG	1075	0.0	20.306976	1
ATTGGAC	1165	0.0	18.103004	3
TTAACGG	345	0.0	17.15942	35
TTGGACC	1720	0.0	15.811047	4
GGACCCT	1675	0.0	15.352239	6
TAACGGC	390	0.0	15.179487	36
GTATTAG	585	0.0	14.863248	1
ATTCGCG	75	0.0041063754	14.8	29
GTATCAA	18860	0.0	14.752916	2
CGAACTA	465	0.0	14.720431	24
TGGACCC	1745	0.0	14.630373	5
TATTGGA	1395	0.0	14.587814	2
TATACCG	90	8.279276E-4	14.388889	5
TAATACT	665	0.0	14.18797	4
ATCCGTC	145	8.922434E-7	14.034483	7
CTAATAC	610	0.0	13.950819	3
TCTAATA	635	0.0	13.692913	2
TCGGTTC	95	0.0012458599	13.631579	13
>>END_MODULE