FastQCFastQC Report
Wed 25 May 2016
SRR2088684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033853
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT308551.0170235670614232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT306601.0105960967785848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225080.7418948775698757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129270.42609183767308434No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG75510.24889142618314072No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG65410.2156004262566446No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA63450.2091399945877404No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55750.18375972731704535No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG52880.17429980951614993No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50990.1680701075497066No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.12040794329850524No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.11773807102717238No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA35310.11638665419847302No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34600.11404639578779854No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33400.11009102945989803No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32100.10580604927133912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98750.022.518481
GTATTGG10950.017.7397251
CGCGAAT700.002593807615.85714235
ATTGGAC12400.015.2177413
TGGACCC14150.015.03533655
ACCGTTA1601.0984877E-815.031258
GTATCAA148450.014.9669912
GGTACGT1001.09430264E-414.88
GGACCCT14050.014.7473316
TTGGACC15050.014.5049844
CGCGCTT908.2795287E-414.38888817
CCCCTAT3550.014.0704221
GTATTAG7850.013.9044591
CGCGATA1102.458442E-413.45454514
CGAATTA1102.458442E-413.45454515
ACGTTTA1551.8902265E-613.12903226
ACGGTGT1703.7370046E-713.05882429
TATTGGA14200.013.0281692
ATACCGT3000.012.956
TGCGCGA1451.3736186E-512.7586212
Produced by FastQC (version 0.11.5)