##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3033853
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.591226404179768	31.0	31.0	34.0	30.0	34.0
2	31.792874275714745	31.0	31.0	34.0	30.0	34.0
3	31.86607162575115	31.0	31.0	34.0	30.0	34.0
4	35.57914737464208	37.0	35.0	37.0	33.0	37.0
5	35.45611669385432	37.0	35.0	37.0	33.0	37.0
6	35.5036315207098	37.0	35.0	37.0	33.0	37.0
7	35.36932672743208	37.0	35.0	37.0	33.0	37.0
8	35.40018913243325	37.0	35.0	37.0	33.0	37.0
9	37.001102558363904	39.0	37.0	39.0	33.0	39.0
10	36.811729177385985	39.0	37.0	39.0	32.0	39.0
11	36.90744014294694	39.0	37.0	39.0	33.0	39.0
12	36.77062929548663	39.0	37.0	39.0	32.0	39.0
13	36.813392408926866	39.0	37.0	39.0	32.0	39.0
14	37.814892151992865	40.0	37.0	41.0	32.0	41.0
15	37.82822305497333	40.0	37.0	41.0	32.0	41.0
16	37.71068967415363	40.0	37.0	41.0	32.0	41.0
17	37.736628966531995	39.0	37.0	41.0	32.0	41.0
18	37.6951055308217	39.0	37.0	41.0	32.0	41.0
19	37.74586606536309	40.0	37.0	41.0	32.0	41.0
20	37.62404935242413	39.0	37.0	41.0	32.0	41.0
21	37.56435661187276	39.0	36.0	41.0	32.0	41.0
22	37.43360044141888	39.0	36.0	41.0	32.0	41.0
23	37.27286556072427	39.0	36.0	41.0	31.0	41.0
24	37.11725749401833	39.0	36.0	41.0	31.0	41.0
25	37.29531622000143	39.0	36.0	41.0	31.0	41.0
26	37.18759049960562	39.0	36.0	41.0	31.0	41.0
27	37.070556813398674	39.0	36.0	41.0	31.0	41.0
28	36.90992674991175	39.0	35.0	41.0	30.0	41.0
29	36.810825376180055	39.0	35.0	41.0	30.0	41.0
30	36.61940344505815	39.0	35.0	40.0	30.0	41.0
31	36.48101539527459	39.0	35.0	40.0	30.0	41.0
32	36.31676683082536	38.0	35.0	40.0	30.0	41.0
33	36.18978539830374	38.0	35.0	40.0	30.0	41.0
34	36.01742141099124	38.0	35.0	40.0	29.0	41.0
35	35.88598030293492	38.0	35.0	40.0	29.0	41.0
36	35.69884697775403	38.0	35.0	40.0	27.0	41.0
37	35.64252420931403	38.0	35.0	40.0	27.0	41.0
38	35.45454377651126	38.0	34.0	40.0	26.0	41.0
39	35.37111850837862	38.0	34.0	40.0	25.0	41.0
40	35.210943641633264	38.0	34.0	40.0	25.0	41.0
41	35.08976011692063	38.0	34.0	40.0	24.0	41.0
42	34.84603538800331	38.0	34.0	40.0	23.0	41.0
43	34.50090627330988	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	2.0
14	8.0
15	10.0
16	47.0
17	102.0
18	201.0
19	466.0
20	1035.0
21	2178.0
22	4125.0
23	7122.0
24	11568.0
25	17641.0
26	25618.0
27	35878.0
28	48786.0
29	63503.0
30	80544.0
31	100607.0
32	124071.0
33	152795.0
34	187101.0
35	232443.0
36	297200.0
37	409144.0
38	562110.0
39	669543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04167209156145	21.544484851441386	13.898761739609663	23.515081317387494
2	18.932262044337676	23.32957463660896	35.022296729604236	22.715866589449128
3	19.715325693103786	24.77812867004433	32.443925265990146	23.06262037086174
4	13.867975805024171	16.77579632236631	36.269456694177336	33.08677117843218
5	12.742871853052867	38.526883141668364	35.051698285974965	13.678546719303803
6	32.17528996955357	37.34656886803678	14.023685392799191	16.45445576961046
7	25.888004461653217	32.97344993313783	22.01718408901156	19.121361516197393
8	26.41551848425088	34.958252756478316	19.32519472762853	19.301034031642274
9	24.551222488367102	14.613199782586697	21.92891349712725	38.906664231918946
10	16.23991010770792	27.91035030372269	34.02874166942169	21.820997919147697
11	32.81839957308413	22.82022233773357	23.46287048185921	20.898507607323097
12	20.525780253690602	27.39466941872266	31.247888411205153	20.831661916381577
13	30.83702473389449	21.3582530201694	26.399103713990097	21.40561853194601
14	20.29847853538059	23.26582731595763	27.990347587704477	28.4453465609573
15	22.732907626045165	29.720952201705224	25.566795754441628	21.979344417807983
16	21.6286023086814	28.629468863521073	27.017459316585214	22.72446951121231
17	21.3146451064043	27.39150512566034	27.494179843255424	23.799669924679936
18	22.365454094183203	26.342970473519976	29.551860291187477	21.739715141109343
19	22.43955129005921	25.55443523466694	30.6204025046698	21.385610970604045
20	23.137640485547585	25.316256258955192	30.495017392075358	21.05108586342186
21	22.09180866706462	26.092430978033544	30.89454235257938	20.92121800232246
22	21.054579770344837	26.349628673505276	30.5012470940418	22.094544462108086
23	21.831809253777294	26.478046233617782	31.23203398450749	20.45811052809744
24	21.897962755611427	26.58368747595879	29.203194749383044	22.31515501904674
25	21.795683574649136	26.723114139017284	30.21566964516738	21.265532641166203
26	21.54102390590447	27.3625979900806	30.01572587729201	21.080652226722915
27	20.720351315637245	27.306860286243268	29.885693209262282	22.087095188857205
28	21.0228709169495	27.038323873964888	30.089361613763092	21.849443595322516
29	21.304064501477164	26.918970695020487	29.61676126035111	22.160203543151233
30	21.431559142779825	27.135527001473044	30.719945890588633	20.712967965158498
31	21.90056670511063	26.839731522918214	30.54871148997661	20.710990281994548
32	21.452687391248027	26.849488093193703	29.864993458812933	21.832831056745334
33	21.114371724668267	26.729475686527987	30.41211950611978	21.744033082683966
34	20.617544752497896	26.96989603649221	30.984362129608783	21.42819708140111
35	20.366444913448344	28.504017828154492	29.930883269558546	21.198653988838615
36	22.028127269185422	26.72822315385749	29.827549324242142	21.416100252714948
37	21.196445575972202	27.03911494723047	30.018659440651867	21.745780036145458
38	21.046438307986577	26.0583159434554	30.996129344434287	21.899116404123735
39	20.622159346547114	25.88981733788684	31.68966986864558	21.79835344692047
40	19.862168668027095	26.29395689244007	31.384546317834122	22.45932812169871
41	20.90513284592233	25.394506589475498	31.403729844524435	22.296630720077736
42	19.71150217232015	27.15560048558714	30.796053731014656	22.33684361107806
43	20.17437891684271	26.78719107352927	30.26985816385962	22.768571845768403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	881.0
1	837.0
2	793.0
3	1524.5
4	2256.0
5	2256.0
6	3507.0
7	4758.0
8	5306.0
9	5854.0
10	9059.0
11	12264.0
12	12264.0
13	23064.5
14	33865.0
15	50372.0
16	66879.0
17	62796.0
18	58713.0
19	58713.0
20	64730.5
21	70748.0
22	58854.5
23	46961.0
24	53031.0
25	59101.0
26	59101.0
27	65385.0
28	71669.0
29	78336.5
30	85004.0
31	94083.5
32	103163.0
33	103163.0
34	114926.5
35	126690.0
36	136798.5
37	146907.0
38	154714.0
39	162521.0
40	162521.0
41	167173.0
42	171825.0
43	180121.0
44	188417.0
45	197247.5
46	206078.0
47	206078.0
48	251852.5
49	297627.0
50	277939.5
51	258252.0
52	234889.5
53	211527.0
54	211527.0
55	190753.5
56	169980.0
57	147001.5
58	124023.0
59	111370.5
60	98718.0
61	98718.0
62	87312.5
63	75907.0
64	66427.0
65	56947.0
66	48481.5
67	40016.0
68	40016.0
69	34012.5
70	28009.0
71	23573.5
72	19138.0
73	15684.0
74	12230.0
75	12230.0
76	9834.5
77	7439.0
78	5748.5
79	4058.0
80	3189.0
81	2320.0
82	2320.0
83	1728.5
84	1137.0
85	862.0
86	587.0
87	444.5
88	302.0
89	302.0
90	238.0
91	174.0
92	124.0
93	74.0
94	49.5
95	25.0
96	25.0
97	17.5
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3033853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.40775565847599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.74825189810926	39.695226402120106
2	12.064745116637967	12.163134478425562
3	3.9111098190084195	5.91450804330128
4	1.665815666554645	3.358801163669915
5	0.9112981984015599	2.296824845851761
6	0.5451169305119541	1.6486872623127011
7	0.34580769269106076	1.2201972774594645
8	0.2580706107913337	1.0407008233122568
9	0.19915868657238578	0.9035228169003444
>10	1.1466439927273115	11.036195545271827
>50	0.12204921760657089	4.281481583765586
>100	0.07236834231759236	7.069892226332376
>500	0.005502476423893907	1.871603159021786
>1k	0.003406294928994245	3.0960485347130744
>5k	3.9303403026856674E-4	1.2018927574311955
>10k+	2.6202268684571116E-4	3.2012830801108385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30855	1.0170235670614232	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30660	1.0105960967785848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22508	0.7418948775698757	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12927	0.42609183767308434	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7551	0.24889142618314072	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6541	0.2156004262566446	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6345	0.2091399945877404	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5575	0.18375972731704535	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5288	0.17429980951614993	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5099	0.1680701075497066	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3653	0.12040794329850524	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3572	0.11773807102717238	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3531	0.11638665419847302	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3460	0.11404639578779854	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3340	0.11009102945989803	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3210	0.10580604927133912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.296138606583773E-5	0.0
3	0.0	0.0	0.0	3.296138606583773E-5	0.0
4	0.0	0.0	0.0	3.296138606583773E-5	0.0
5	0.0	0.0	0.0	6.592277213167546E-5	0.0
6	0.0	0.0	0.0	6.592277213167546E-5	0.0
7	0.0	0.0	0.0	6.592277213167546E-5	0.0
8	0.0	0.0	0.0	9.888415819751319E-5	0.0
9	3.296138606583773E-5	0.0	0.0	2.3072970246086413E-4	0.0
10	6.592277213167546E-5	0.0	0.0	2.6369108852670185E-4	0.0
11	6.592277213167546E-5	0.0	0.0	4.6145940492172826E-4	0.0
12	6.592277213167546E-5	0.0	0.0	7.910732655801055E-4	0.0
13	6.592277213167546E-5	0.0	0.0	8.56996037711781E-4	0.0
14	6.592277213167546E-5	0.0	0.0	9.88841581975132E-4	0.0
15	6.592277213167546E-5	0.0	0.0	0.001351416828699347	0.0
16	6.592277213167546E-5	0.0	0.0	0.001845837619686913	0.0
17	6.592277213167546E-5	0.0	0.0	0.0026039494992011808	0.0
18	6.592277213167546E-5	0.0	0.0	0.003164293062320422	0.0
19	6.592277213167546E-5	0.0	0.0	0.003988327713966366	0.0
20	6.592277213167546E-5	0.0	0.0	0.006625238599233384	0.0
21	6.592277213167546E-5	0.0	0.0	0.01133871680664818	3.296138606583773E-5
22	6.592277213167546E-5	0.0	0.0	0.01875502867146167	3.296138606583773E-5
23	6.592277213167546E-5	0.0	0.0	0.023468506878876465	3.296138606583773E-5
24	6.592277213167546E-5	0.0	0.0	0.031016664287953306	3.296138606583773E-5
25	6.592277213167546E-5	0.0	0.0	0.03744413457079166	3.296138606583773E-5
26	6.592277213167546E-5	0.0	0.0	0.049508001870888274	3.296138606583773E-5
27	6.592277213167546E-5	0.0	0.0	0.10775077104922355	3.296138606583773E-5
28	6.592277213167546E-5	0.0	0.0	0.2317515054289051	3.296138606583773E-5
29	6.592277213167546E-5	0.0	0.0	0.3809677001489525	3.296138606583773E-5
30	6.592277213167546E-5	0.0	0.0	0.5915250343375239	3.296138606583773E-5
31	9.888415819751319E-5	0.0	0.0	0.8631927782921586	3.296138606583773E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9875	0.0	22.51848	1
GTATTGG	1095	0.0	17.739725	1
CGCGAAT	70	0.0025938076	15.857142	35
ATTGGAC	1240	0.0	15.217741	3
TGGACCC	1415	0.0	15.0353365	5
ACCGTTA	160	1.0984877E-8	15.03125	8
GTATCAA	14845	0.0	14.966991	2
GGTACGT	100	1.09430264E-4	14.8	8
GGACCCT	1405	0.0	14.747331	6
TTGGACC	1505	0.0	14.504984	4
CGCGCTT	90	8.2795287E-4	14.388888	17
CCCCTAT	355	0.0	14.070422	1
GTATTAG	785	0.0	13.904459	1
CGCGATA	110	2.458442E-4	13.454545	14
CGAATTA	110	2.458442E-4	13.454545	15
ACGTTTA	155	1.8902265E-6	13.129032	26
ACGGTGT	170	3.7370046E-7	13.058824	29
TATTGGA	1420	0.0	13.028169	2
ATACCGT	300	0.0	12.95	6
TGCGCGA	145	1.3736186E-5	12.75862	12
>>END_MODULE