FastQCFastQC Report
Wed 25 May 2016
SRR2088683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621228
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT193941.1962537039824135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT184421.137532783791052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139390.85978036402036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76360.47100099430801834No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG52040.32099124860907907No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG44570.2749150643832946No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA43440.26794503919251333No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41120.2536348989778119No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG36810.22705011263067254No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33920.2092241190011522No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA23230.1432864470635839No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC22370.13798182612192733No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA22310.1376117362887885No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21880.13495942581796022No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.13058002945915073No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.12441186557350355No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG19800.12212964493581409No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18940.1168250239941575No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.11090358666393622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.022.7716481
ACGTTTA656.904366E-519.92307726
GTACCGT609.239047E-418.56
GTATTGG5850.018.341881
GTATTAG3450.017.6956521
GGACCCT7800.017.5512836
TAGGTCG953.6082365E-617.52631621
TTGGACC8150.017.4785274
ATTGGAC6950.016.5035973
TGGACCC8550.016.228075
GTATCAA87000.015.4379312
TAATACT4400.015.1363644
ACGGGTA908.276599E-414.38888817
TAGTACT2700.014.3888884
GTATAGA1551.2109376E-714.322581
GAACTAT1051.656293E-414.0952381
TATTGGA8150.013.8466252
TAGGACC2950.013.796614
GACCCTC9400.013.7765967
GATACTG5800.013.71551736
Produced by FastQC (version 0.11.5)