##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1621228
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56046527693822	31.0	31.0	34.0	30.0	34.0
2	31.763161011282804	31.0	31.0	34.0	30.0	34.0
3	31.82799581551762	31.0	31.0	34.0	30.0	34.0
4	35.55412008674906	37.0	35.0	37.0	33.0	37.0
5	35.43634948323123	37.0	35.0	37.0	33.0	37.0
6	35.484805961900484	37.0	35.0	37.0	33.0	37.0
7	35.34677047275275	37.0	35.0	37.0	33.0	37.0
8	35.381634168667205	37.0	35.0	37.0	33.0	37.0
9	36.975854722469634	39.0	37.0	39.0	33.0	39.0
10	36.79200951377598	39.0	37.0	39.0	32.0	39.0
11	36.8835765234748	39.0	37.0	39.0	33.0	39.0
12	36.7360889399887	39.0	35.0	39.0	32.0	39.0
13	36.7810585556134	39.0	37.0	39.0	32.0	39.0
14	37.744931002918776	40.0	37.0	41.0	32.0	41.0
15	37.7590184724172	40.0	37.0	41.0	32.0	41.0
16	37.63931908405234	39.0	36.0	41.0	32.0	41.0
17	37.677170638553	39.0	37.0	41.0	32.0	41.0
18	37.65309567809093	39.0	37.0	41.0	32.0	41.0
19	37.69785372569435	40.0	37.0	41.0	32.0	41.0
20	37.578956198634614	39.0	37.0	41.0	32.0	41.0
21	37.51820595252487	39.0	36.0	41.0	32.0	41.0
22	37.38444376731712	39.0	36.0	41.0	31.0	41.0
23	37.205036552539184	39.0	36.0	41.0	31.0	41.0
24	37.06196229031327	39.0	36.0	41.0	31.0	41.0
25	37.234312508789635	39.0	36.0	41.0	31.0	41.0
26	37.11083265277925	39.0	36.0	41.0	31.0	41.0
27	37.00815863037155	39.0	36.0	41.0	31.0	41.0
28	36.83924778007782	39.0	35.0	41.0	30.0	41.0
29	36.72511886051807	39.0	35.0	41.0	30.0	41.0
30	36.51759283703464	39.0	35.0	40.0	30.0	41.0
31	36.35645634050238	38.0	35.0	40.0	30.0	41.0
32	36.18504985110052	38.0	35.0	40.0	30.0	41.0
33	36.04525273434705	38.0	35.0	40.0	30.0	41.0
34	35.85930603221755	38.0	35.0	40.0	29.0	41.0
35	35.712635113629915	38.0	35.0	40.0	27.0	41.0
36	35.514597576651774	38.0	34.0	40.0	27.0	41.0
37	35.43667207820245	38.0	34.0	40.0	26.0	41.0
38	35.24306019881226	38.0	34.0	40.0	25.0	41.0
39	35.14736668747394	38.0	34.0	40.0	25.0	41.0
40	34.96906480766432	38.0	34.0	40.0	24.0	41.0
41	34.81969223329476	38.0	33.0	40.0	23.0	41.0
42	34.54466305787958	38.0	33.0	40.0	22.0	41.0
43	34.18989185975076	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	1.0
15	8.0
16	20.0
17	45.0
18	113.0
19	264.0
20	609.0
21	1195.0
22	2249.0
23	3910.0
24	6597.0
25	9841.0
26	14271.0
27	20145.0
28	27129.0
29	35595.0
30	44702.0
31	55266.0
32	68075.0
33	84082.0
34	102061.0
35	126607.0
36	161962.0
37	223794.0
38	297492.0
39	335190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26995092608813	21.518503258024165	13.974530417683386	23.237015398204324
2	18.95754329434231	23.538700293851328	34.94425213480152	22.559504277004837
3	19.755765382783913	24.61085054045452	32.84929695268031	22.78408712408125
4	13.72200578820499	16.580456296091604	36.375512882827095	33.32202503287631
5	12.57935342838885	38.5047630561525	35.14410064469649	13.771782870762165
6	32.10449116348842	37.245038945786774	13.692151874998457	16.958318015726352
7	25.74702632819073	33.16381162920946	21.78151376610816	19.307648276491644
8	26.692235762027305	34.62875055205067	19.214015548707522	19.464998137214508
9	24.437340090351263	14.47618718650307	21.981053867808846	39.10541885533682
10	16.268285521839	27.82458728815441	33.88536344055247	22.021763749454117
11	32.72587199332852	22.628340985968663	23.654538411623783	20.991248609079044
12	20.7220699371094	27.53727421436097	31.139050152106922	20.601605696422713
13	31.284063685058488	21.11263807434858	26.46543237595206	21.13786586464088
14	20.277407002593094	23.2853738030678	28.133488935547625	28.303730258791482
15	22.68064701571895	29.613786586464087	25.76997189784534	21.935594499971625
16	21.49574273328613	28.573710792066258	27.09316641459437	22.837380060053246
17	21.387183048898738	27.10698310169822	27.394172812213952	24.111661037189094
18	22.6654116509214	25.88790719133891	29.680279393151366	21.766401764588323
19	22.406718857557358	25.051504168445156	31.118880256200853	21.422896717796633
20	23.457650620393924	24.71663455109337	30.777534066769142	21.048180761743566
21	22.17189685843077	25.689785767331923	31.352036851078317	20.786280523158986
22	20.80793077839761	26.028541327931663	30.957706133868896	22.20582175980183
23	21.86089803531644	26.12007687999467	31.75136378103512	20.26766130365377
24	21.88587909905331	26.10188079653201	29.44521066746935	22.567029436945326
25	21.727357287192177	26.37950985302499	30.634309301344416	21.25882355843842
26	21.467615905967573	27.02223252990943	30.434830881282583	21.075320682840413
27	20.42032335982354	27.206167176979424	30.207410678818775	22.16609878437826
28	21.01771003214847	26.728319520758337	30.30579289279484	21.948177554298347
29	21.24827599819396	26.68588255322509	29.711737028968166	22.35410441961279
30	21.349680612474	26.94216976267373	31.15064630021194	20.557503324640333
31	22.043845776164734	26.72548216537094	30.75865948527906	20.472012573185264
32	21.527755503852635	26.52378320631028	30.067578403531147	21.880882886305937
33	21.076862723811825	26.593976911328944	30.665026757494935	21.664133607364295
34	20.528327909461222	27.01285692080324	31.171371330867714	21.28744383886782
35	20.161383839904072	28.937755824597154	30.053144900038735	20.847715435460035
36	22.181642557370093	26.884990883453774	29.813141643248205	21.120224915927928
37	21.270728114737718	27.43247710994382	29.942858129763366	21.3539366455551
38	21.221136077097114	26.228266474548924	30.948638932956996	21.601958515396973
39	20.670133997192252	26.09423227331381	31.618439849299417	21.61719388019452
40	19.733498311156726	26.60384597354598	31.302259768521147	22.360395946776148
41	21.189184988169462	25.63057139402971	31.28992344074985	21.890320177050977
42	19.91990022378099	27.478121522697606	30.66157258571897	21.94040566780243
43	20.349081066944315	27.206413903534855	29.925278862689268	22.51922616683156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	580.0
1	526.0
2	472.0
3	813.0
4	1154.0
5	1154.0
6	1901.5
7	2649.0
8	2899.5
9	3150.0
10	4876.5
11	6603.0
12	6603.0
13	12731.5
14	18860.0
15	29329.0
16	39798.0
17	36869.0
18	33940.0
19	33940.0
20	37068.5
21	40197.0
22	32826.5
23	25456.0
24	28426.0
25	31396.0
26	31396.0
27	34666.0
28	37936.0
29	40778.0
30	43620.0
31	48123.0
32	52626.0
33	52626.0
34	58345.0
35	64064.0
36	69824.5
37	75585.0
38	79158.5
39	82732.0
40	82732.0
41	85231.0
42	87730.0
43	91663.5
44	95597.0
45	101318.0
46	107039.0
47	107039.0
48	136734.0
49	166429.0
50	155186.5
51	143944.0
52	130909.0
53	117874.0
54	117874.0
55	104959.0
56	92044.0
57	79211.0
58	66378.0
59	58987.0
60	51596.0
61	51596.0
62	45778.0
63	39960.0
64	34952.5
65	29945.0
66	25625.0
67	21305.0
68	21305.0
69	18173.0
70	15041.0
71	12736.0
72	10431.0
73	8402.0
74	6373.0
75	6373.0
76	5192.5
77	4012.0
78	3111.5
79	2211.0
80	1705.5
81	1200.0
82	1200.0
83	924.0
84	648.0
85	495.0
86	342.0
87	247.5
88	153.0
89	153.0
90	130.0
91	107.0
92	71.0
93	35.0
94	23.0
95	11.0
96	11.0
97	7.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1621228.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.730553709593146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57555968109112	45.19406104225672
2	10.304476751877784	11.279394366358018
3	2.9601402653549895	4.860303473428218
4	1.2068640938697466	2.6420936043887044
5	0.6543288155278111	1.7905889190989663
6	0.3995724609677817	1.312129322152289
7	0.2928616998697358	1.1219938095942328
8	0.20578106042920485	0.90100091041901
9	0.15790507917499086	0.7778009175123956
>10	1.0865113101467128	11.317203738734399
>50	0.08999086208701733	3.423285769926111
>100	0.05685288638680938	5.7402438853967315
>500	0.0054252048688782925	2.151836352153403
>1k	0.0031647028401790038	3.491027555159469
>5k	2.2605020286992888E-4	0.7942729477850198
>10k+	3.390753043048933E-4	3.202763385636246
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19394	1.1962537039824135	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18442	1.137532783791052	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13939	0.85978036402036	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7636	0.47100099430801834	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5204	0.32099124860907907	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4457	0.2749150643832946	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4344	0.26794503919251333	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4112	0.2536348989778119	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3681	0.22705011263067254	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3392	0.2092241190011522	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2323	0.1432864470635839	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2237	0.13798182612192733	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2231	0.1376117362887885	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2188	0.13495942581796022	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2117	0.13058002945915073	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2017	0.12441186557350355	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1980	0.12212964493581409	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1894	0.1168250239941575	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.11090358666393622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.168163885647176E-5	0.0
8	0.0	0.0	0.0	6.168163885647176E-5	0.0
9	0.0	0.0	0.0	6.168163885647176E-5	0.0
10	6.168163885647176E-5	0.0	0.0	6.168163885647176E-5	0.0
11	6.168163885647176E-5	0.0	0.0	1.850449165694153E-4	0.0
12	6.168163885647176E-5	0.0	0.0	3.700898331388306E-4	0.0
13	1.2336327771294352E-4	0.0	0.0	3.700898331388306E-4	0.0
14	1.2336327771294352E-4	0.0	0.0	6.784980274211894E-4	0.0
15	1.850449165694153E-4	0.0	0.0	0.0010485878605600199	0.0
16	1.850449165694153E-4	0.0	0.0	0.0018504491656941527	0.0
17	1.850449165694153E-4	0.0	0.0	0.0028990370262541726	0.0
18	1.850449165694153E-4	0.0	0.0	0.0032074452205365315	0.0
19	1.850449165694153E-4	0.0	0.0	0.0041943514422400794	0.0
20	1.850449165694153E-4	0.0	0.0	0.0065999353576424785	0.0
21	1.850449165694153E-4	0.0	0.0	0.010424196966743727	0.0
22	1.850449165694153E-4	0.0	0.0	0.01714749560209915	0.0
23	1.850449165694153E-4	0.0	0.0	0.021650255238621588	0.0
24	1.850449165694153E-4	0.0	0.0	0.02800346404083818	0.0
25	1.850449165694153E-4	0.0	0.0	0.03275295023278651	0.0
26	1.850449165694153E-4	0.0	0.0	0.041943514422400796	0.0
27	1.850449165694153E-4	0.0	0.0	0.09264582156242059	0.0
28	1.850449165694153E-4	0.0	0.0	0.19713451778528374	0.0
29	1.850449165694153E-4	0.0	0.0	0.3228416977747732	0.0
30	1.850449165694153E-4	0.0	0.0	0.5043090792905132	0.0
31	1.850449165694153E-4	0.0	0.0	0.7214284480652937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5890	0.0	22.771648	1
ACGTTTA	65	6.904366E-5	19.923077	26
GTACCGT	60	9.239047E-4	18.5	6
GTATTGG	585	0.0	18.34188	1
GTATTAG	345	0.0	17.695652	1
GGACCCT	780	0.0	17.551283	6
TAGGTCG	95	3.6082365E-6	17.526316	21
TTGGACC	815	0.0	17.478527	4
ATTGGAC	695	0.0	16.503597	3
TGGACCC	855	0.0	16.22807	5
GTATCAA	8700	0.0	15.437931	2
TAATACT	440	0.0	15.136364	4
ACGGGTA	90	8.276599E-4	14.388888	17
TAGTACT	270	0.0	14.388888	4
GTATAGA	155	1.2109376E-7	14.32258	1
GAACTAT	105	1.656293E-4	14.095238	1
TATTGGA	815	0.0	13.846625	2
TAGGACC	295	0.0	13.79661	4
GACCCTC	940	0.0	13.776596	7
GATACTG	580	0.0	13.715517	36
>>END_MODULE