FastQCFastQC Report
Wed 25 May 2016
SRR2088682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574132
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT120070.7627695771383849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113770.7227475205383029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87450.5555442618535167No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47460.30149949305394974No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG31700.2013808244797768No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG26420.1678385294244701No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25430.16154934910160013No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA23470.1490980426037969No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22160.1407759959139386No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20990.13334332825963768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.022.258191
GTATTGG5150.019.3980581
TACCCCG1551.8189894E-1219.0967755
TTAACGG1800.018.535
GTACGAC500.00703600918.53
TAGGACG650.001580284817.0769234
CTAATAC4300.016.3488373
TAACGGC2003.6379788E-1215.72536
AGTACCG1557.214112E-915.51612955
ATTGGAC5050.015.0198023
CTCTAAT3450.015.0144931
GGACCCT5950.014.9243716
GTATCAA56900.014.8910372
AATGCGA1001.0937714E-414.820
TGGACCC6300.014.6825395
ATAGTAC2151.2732926E-1114.6279073
CGGTTGG2059.276846E-1114.43902533
CAATGCG908.276411E-414.38888819
TACTCCG2450.014.3469385
GGTTAGA1551.2108649E-714.3225811
Produced by FastQC (version 0.11.5)