##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1574132
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617699151024183	31.0	31.0	34.0	30.0	34.0
2	31.832801188210393	31.0	31.0	34.0	30.0	34.0
3	31.922125971646597	31.0	31.0	34.0	30.0	34.0
4	35.63604068782033	37.0	35.0	37.0	33.0	37.0
5	35.4967823537035	37.0	35.0	37.0	33.0	37.0
6	35.537910416661376	37.0	35.0	37.0	33.0	37.0
7	35.411846020537034	37.0	35.0	37.0	33.0	37.0
8	35.424031148594906	37.0	35.0	37.0	33.0	37.0
9	37.04225503325007	39.0	37.0	39.0	33.0	39.0
10	36.84624478760358	39.0	37.0	39.0	32.0	39.0
11	36.93839144366546	39.0	37.0	39.0	33.0	39.0
12	36.79398805182793	39.0	37.0	39.0	32.0	39.0
13	36.83288949084321	39.0	37.0	39.0	32.0	39.0
14	37.860805828227875	40.0	37.0	41.0	32.0	41.0
15	37.864113047698666	40.0	37.0	41.0	33.0	41.0
16	37.764442880266714	40.0	37.0	41.0	32.0	41.0
17	37.752844107101566	40.0	37.0	41.0	32.0	41.0
18	37.7099328391774	39.0	37.0	41.0	32.0	41.0
19	37.74792329995197	40.0	37.0	41.0	32.0	41.0
20	37.62076306180168	39.0	37.0	41.0	32.0	41.0
21	37.55734716021274	39.0	36.0	41.0	32.0	41.0
22	37.41966048590589	39.0	36.0	41.0	32.0	41.0
23	37.25419215161117	39.0	36.0	41.0	31.0	41.0
24	37.10502422922601	39.0	36.0	41.0	31.0	41.0
25	37.29796357611687	39.0	36.0	41.0	31.0	41.0
26	37.19477146770411	39.0	36.0	41.0	31.0	41.0
27	37.09543418213975	39.0	36.0	41.0	31.0	41.0
28	36.93809731331299	39.0	36.0	41.0	30.0	41.0
29	36.849895053273805	39.0	36.0	41.0	30.0	41.0
30	36.68083362767544	39.0	35.0	40.0	30.0	41.0
31	36.58000282060208	39.0	35.0	40.0	30.0	41.0
32	36.40975216817903	39.0	35.0	40.0	30.0	41.0
33	36.29612827894992	38.0	35.0	40.0	30.0	41.0
34	36.13655589239022	38.0	35.0	40.0	29.0	41.0
35	36.01911402601561	38.0	35.0	40.0	29.0	41.0
36	35.84289754607619	38.0	35.0	40.0	28.0	41.0
37	35.80836295812549	38.0	35.0	40.0	27.0	41.0
38	35.65147331989947	38.0	35.0	40.0	27.0	41.0
39	35.591397671859795	38.0	35.0	40.0	26.0	41.0
40	35.46365933733639	38.0	34.0	40.0	26.0	41.0
41	35.362172295588934	38.0	34.0	40.0	25.0	41.0
42	35.160618677467966	38.0	34.0	40.0	24.0	41.0
43	34.82607875324306	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	2.0
15	11.0
16	20.0
17	48.0
18	108.0
19	281.0
20	522.0
21	1122.0
22	1895.0
23	3403.0
24	5415.0
25	8558.0
26	12511.0
27	17375.0
28	23781.0
29	31525.0
30	40513.0
31	50340.0
32	62742.0
33	77694.0
34	97227.0
35	120000.0
36	155654.0
37	212424.0
38	301235.0
39	349723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.33344598801117	21.015200758259155	13.295898946212898	24.355454307516776
2	18.818243959210537	22.794657627187558	34.89777223257008	23.489326181031835
3	19.449258384938492	24.517003656618378	31.98213364571713	24.051604312725996
4	13.883714961642351	16.46831396604605	36.66007679152701	32.98789428078459
5	12.973308464601443	38.11560911029062	35.33464792025065	13.576434504857279
6	32.53691558268303	37.53008006952403	14.362073828624283	15.57093051916866
7	26.724378895797813	32.35224237865694	22.07318064812862	18.850198077416632
8	26.613270043427107	34.664881979401976	19.50198585633225	19.21986212083866
9	24.987231058132355	14.370141767018268	21.393377429592945	39.249249745256435
10	16.00837795051495	27.519039064068323	34.46839273961777	22.004190245798956
11	33.50722811047612	22.543280995494662	23.360620329171887	20.58887056485733
12	20.78967964567139	26.760779909181696	31.138430576343023	21.311109868803886
13	30.572467874358694	21.169317439706454	25.89141190192436	22.36680278401049
14	20.681365984555296	22.617671199111637	27.371275090017868	29.3296877263152
15	23.20955294727507	29.474974144480893	24.743477675315667	22.57199523292837
16	22.304546251521472	28.31585915285376	26.144503764614402	23.23509083101036
17	21.82694970942716	27.225607509408363	26.98344230344088	23.964000477723594
18	22.631138938792937	26.535894067333615	28.711950459046637	22.12101653482681
19	22.852086102054976	25.703117654682075	29.530433280055295	21.914362963207658
20	23.53900435287511	25.45237629372886	29.31704583859549	21.691573514800538
21	22.598994239364934	26.164070103396664	29.505530667059688	21.731404990178717
22	21.83177776704876	26.34073889610274	29.20091834738129	22.62656498946721
23	22.458472351746867	26.474717495102063	29.61625835698658	21.45055179616449
24	22.564689619422005	26.61657345127346	27.960869863518433	22.857867065786095
25	22.534323678065117	26.565243575507008	28.918222868221978	21.982209878205893
26	22.24540254565691	27.18348905936732	28.800443673084597	21.770664721891176
27	21.58522919297746	27.200895477634656	28.4948149202227	22.719060409165177
28	21.769775342855617	26.85848454894507	28.875469147441258	22.496270960758054
29	22.23314182038101	26.659390699128156	28.49233736433793	22.6151301161529
30	22.149222555668775	26.969593401315773	29.335214581750453	21.545969461265003
31	22.616527711780208	26.73428911933688	29.243417959866136	21.405765209016778
32	22.002602068949745	26.628453014105553	28.696576907146287	22.672368009798415
33	21.816785377592222	26.685309745307258	29.167312525252008	22.33059235184851
34	21.61559513433435	26.677940604726924	29.54237636996135	22.164087890977378
35	21.355070603990008	28.03856347498177	28.84631022048977	21.760055700538455
36	22.75832014087764	26.64103137475129	28.64003781131443	21.960610673056642
37	21.998726917437676	26.7201861089159	28.88601464172001	22.39507233192642
38	21.85572747393484	26.079007351353	29.656915684326346	22.408349490385813
39	21.362249163348434	25.885440356971333	30.301969593401317	22.450340886278912
40	20.731806481286196	26.180587142628447	30.221417263609407	22.866189112475954
41	21.561978283905034	25.386054028505868	30.224466563159886	22.827501124429208
42	20.618220073030724	26.61905100715823	29.854167248998177	22.908561670812865
43	20.882619754887138	26.23655449479459	29.661171998282228	23.21965375203604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	377.5
2	387.0
3	708.5
4	1030.0
5	1030.0
6	1509.5
7	1989.0
8	2165.0
9	2341.0
10	3650.0
11	4959.0
12	4959.0
13	8814.0
14	12669.0
15	19350.0
16	26031.0
17	24609.5
18	23188.0
19	23188.0
20	25973.5
21	28759.0
22	24875.0
23	20991.0
24	24066.0
25	27141.0
26	27141.0
27	30980.5
28	34820.0
29	38635.5
30	42451.0
31	47758.0
32	53065.0
33	53065.0
34	59818.0
35	66571.0
36	73085.5
37	79600.0
38	83362.5
39	87125.0
40	87125.0
41	90101.5
42	93078.0
43	97309.0
44	101540.0
45	104815.5
46	108091.0
47	108091.0
48	126833.5
49	145576.0
50	138719.5
51	131863.0
52	122071.0
53	112279.0
54	112279.0
55	103410.5
56	94542.0
57	82956.0
58	71370.0
59	64488.5
60	57607.0
61	57607.0
62	51125.5
63	44644.0
64	38936.5
65	33229.0
66	28279.0
67	23329.0
68	23329.0
69	19755.5
70	16182.0
71	13663.0
72	11144.0
73	9024.0
74	6904.0
75	6904.0
76	5500.5
77	4097.0
78	3215.0
79	2333.0
80	1848.5
81	1364.0
82	1364.0
83	1029.0
84	694.0
85	541.0
86	388.0
87	288.5
88	189.0
89	189.0
90	152.0
91	115.0
92	81.0
93	47.0
94	40.0
95	33.0
96	33.0
97	20.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1574132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.02742331194047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87398006660096	50.34738906315111
2	9.64192286524576	11.575595707463702
3	2.6238688852772234	4.725122648746956
4	1.135045951576342	2.7253553525510945
5	0.618603568901345	1.8566589146359083
6	0.40657777424317876	1.4643489698233112
7	0.25680187346339817	1.079060833598074
8	0.2021394442542379	0.9707127990631624
9	0.16434056468562058	0.8878446579336372
>10	0.9523511803866921	10.758178259106389
>50	0.07354357334461067	3.0579551354447445
>100	0.04521257801113044	5.084695419573998
>500	0.003599941817434696	1.4248302638033294
>1k	0.0016940902670280922	2.0002155960457926
>5k	1.0588064168925576E-4	0.555809646576863
>10k+	2.1176128337851153E-4	1.4862267324818028
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12007	0.7627695771383849	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11377	0.7227475205383029	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8745	0.5555442618535167	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4746	0.30149949305394974	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3170	0.2013808244797768	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2642	0.1678385294244701	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2543	0.16154934910160013	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2347	0.1490980426037969	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2216	0.1407759959139386	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2099	0.13334332825963768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.352707396838384E-5	0.0
2	0.0	6.352707396838384E-5	0.0	6.352707396838384E-5	0.0
3	0.0	6.352707396838384E-5	0.0	6.352707396838384E-5	0.0
4	0.0	6.352707396838384E-5	0.0	1.270541479367677E-4	0.0
5	0.0	6.352707396838384E-5	0.0	1.9058122190515153E-4	0.0
6	0.0	6.352707396838384E-5	0.0	1.9058122190515153E-4	0.0
7	0.0	6.352707396838384E-5	0.0	1.9058122190515153E-4	0.0
8	0.0	6.352707396838384E-5	0.0	1.9058122190515153E-4	0.0
9	0.0	6.352707396838384E-5	0.0	3.8116244381030306E-4	0.0
10	0.0	1.270541479367677E-4	0.0	3.8116244381030306E-4	0.0
11	0.0	1.270541479367677E-4	0.0	4.446895177786869E-4	0.0
12	0.0	1.270541479367677E-4	0.0	6.352707396838384E-4	0.0
13	6.352707396838384E-5	1.270541479367677E-4	0.0	6.352707396838384E-4	0.0
14	6.352707396838384E-5	1.270541479367677E-4	0.0	8.2585196158899E-4	0.0
15	6.352707396838384E-5	1.270541479367677E-4	0.0	0.0010799602574625253	0.0
16	6.352707396838384E-5	1.270541479367677E-4	0.0	0.0013340685533360607	0.0
17	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.0018422851450831316	0.0
18	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.0025410829587353538	0.0
19	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.00330340784635596	0.0
20	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.0046374763996920206	0.0
21	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.007686775950174445	0.0
22	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.011752508684151012	0.0
23	6.352707396838384E-5	1.9058122190515153E-4	0.0	0.013975956273044446	0.0
24	6.352707396838384E-5	2.541082958735354E-4	0.0	0.018041689007021012	0.0
25	6.352707396838384E-5	2.541082958735354E-4	0.0	0.02293327370258657	0.0
26	6.352707396838384E-5	3.176353698419192E-4	0.0	0.030683576726729396	0.0
27	6.352707396838384E-5	3.176353698419192E-4	0.0	0.07966295075635334	0.0
28	6.352707396838384E-5	3.176353698419192E-4	0.0	0.17508061585686588	0.0
29	6.352707396838384E-5	3.176353698419192E-4	0.0	0.2948291502872694	0.0
30	6.352707396838384E-5	3.176353698419192E-4	0.0	0.4530750915425136	0.0
31	6.352707396838384E-5	3.176353698419192E-4	0.0	0.6550911867619742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3815	0.0	22.25819	1
GTATTGG	515	0.0	19.398058	1
TACCCCG	155	1.8189894E-12	19.096775	5
TTAACGG	180	0.0	18.5	35
GTACGAC	50	0.007036009	18.5	3
TAGGACG	65	0.0015802848	17.076923	4
CTAATAC	430	0.0	16.348837	3
TAACGGC	200	3.6379788E-12	15.725	36
AGTACCG	155	7.214112E-9	15.5161295	5
ATTGGAC	505	0.0	15.019802	3
CTCTAAT	345	0.0	15.014493	1
GGACCCT	595	0.0	14.924371	6
GTATCAA	5690	0.0	14.891037	2
AATGCGA	100	1.0937714E-4	14.8	20
TGGACCC	630	0.0	14.682539	5
ATAGTAC	215	1.2732926E-11	14.627907	3
CGGTTGG	205	9.276846E-11	14.439025	33
CAATGCG	90	8.276411E-4	14.388888	19
TACTCCG	245	0.0	14.346938	5
GGTTAGA	155	1.2108649E-7	14.322581	1
>>END_MODULE