FastQCFastQC Report
Wed 25 May 2016
SRR2088681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500950
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235001.5656750724541126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT229811.5310969719177854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT183741.2241580332456112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.6266031513374862No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG28510.18994636730070955No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.17275725373929846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.1683600386421933No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.15410240181218562No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22190.14783970152236917No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG20970.13971151603984144No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA20320.1353809254139045No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19560.13031746560511676No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16720.11139611579333089No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.1052000399746827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51900.030.298651
AGTCGTG2300.019.30434820
GTATCAA84200.018.6538012
TACGCGC500.00703586218.49999817
GAGTCGT2500.017.7619
TGAGTCG2550.017.41176418
TTGGACC4400.016.3977284
TATACCG1059.34683E-615.8571425
GTATTGG3750.015.7866681
GGTGCTC3500.015.3285718
CTAGCAC1455.3483745E-815.3103453
TATACGT855.3655106E-415.2352934
GTGCTCG3000.014.89
ATACGTC750.004105183814.85
TATACTG3000.014.85
ACGGTGT1001.093718E-414.79999929
GGACCCT5200.014.5865386
ATTGGAC3950.014.5189873
AGACGCG908.276096E-414.38888714
ACCGTCC908.276096E-414.3888878
Produced by FastQC (version 0.11.5)