##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1500950
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.601107298710815	31.0	31.0	34.0	30.0	34.0
2	31.782876844665047	31.0	31.0	34.0	30.0	34.0
3	31.848898364369234	31.0	31.0	34.0	30.0	34.0
4	35.54507278723475	37.0	35.0	37.0	33.0	37.0
5	35.462074019787465	37.0	35.0	37.0	33.0	37.0
6	35.53297444951531	37.0	35.0	37.0	33.0	37.0
7	35.40039708184816	37.0	35.0	37.0	33.0	37.0
8	35.44201205902928	37.0	35.0	37.0	33.0	37.0
9	37.02030980379093	39.0	37.0	39.0	33.0	39.0
10	36.849259469002966	39.0	37.0	39.0	32.0	39.0
11	36.946179419700854	39.0	37.0	39.0	33.0	39.0
12	36.820389753156334	39.0	37.0	39.0	32.0	39.0
13	36.858802758253105	39.0	37.0	39.0	32.0	39.0
14	37.82191545354609	40.0	37.0	41.0	32.0	41.0
15	37.83185982211266	40.0	37.0	41.0	32.0	41.0
16	37.71459142543056	39.0	37.0	41.0	32.0	41.0
17	37.7971084979513	40.0	37.0	41.0	32.0	41.0
18	37.78524800959392	40.0	37.0	41.0	32.0	41.0
19	37.850530663912856	40.0	37.0	41.0	32.0	41.0
20	37.737127819047934	40.0	37.0	41.0	32.0	41.0
21	37.66975115760019	40.0	37.0	41.0	32.0	41.0
22	37.518829408041576	39.0	36.0	41.0	32.0	41.0
23	37.341557680135914	39.0	36.0	41.0	31.0	41.0
24	37.19273993137679	39.0	36.0	41.0	31.0	41.0
25	37.35581198574236	39.0	36.0	41.0	31.0	41.0
26	37.21573336886638	39.0	36.0	41.0	31.0	41.0
27	37.07339751490723	39.0	36.0	41.0	31.0	41.0
28	36.89853359538959	39.0	35.0	41.0	31.0	41.0
29	36.77919251140944	39.0	35.0	41.0	30.0	41.0
30	36.56788833738632	39.0	35.0	40.0	30.0	41.0
31	36.3995096438922	38.0	35.0	40.0	30.0	41.0
32	36.169354741996735	38.0	35.0	40.0	30.0	41.0
33	35.994240314467504	38.0	35.0	40.0	30.0	41.0
34	35.78549052266898	38.0	35.0	40.0	29.0	41.0
35	35.624182684299946	38.0	35.0	40.0	27.0	41.0
36	35.398023918185146	38.0	34.0	40.0	26.0	41.0
37	35.29086578500283	38.0	34.0	40.0	25.0	41.0
38	35.06916619474333	38.0	34.0	40.0	25.0	41.0
39	34.92270761850828	38.0	34.0	40.0	24.0	41.0
40	34.718001932109665	38.0	33.0	40.0	23.0	41.0
41	34.53692328192145	38.0	33.0	40.0	22.0	41.0
42	34.24223325227356	38.0	33.0	40.0	21.0	41.0
43	33.86538192478097	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	20.0
17	50.0
18	84.0
19	233.0
20	534.0
21	1103.0
22	2059.0
23	3642.0
24	5833.0
25	9129.0
26	12987.0
27	18370.0
28	25045.0
29	32930.0
30	41462.0
31	50828.0
32	62594.0
33	77686.0
34	94710.0
35	116354.0
36	150806.0
37	213711.0
38	268971.0
39	311801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83733635364269	21.498517605516508	14.20027316033179	21.46387288050901
2	18.412671974416202	24.648056231053665	35.03674339584929	21.902528398680836
3	20.000266497884674	26.336253705986206	32.85412572037709	20.809354075752022
4	13.307505246677104	17.43329224824278	37.21123288583897	32.047969619241144
5	12.143309237482928	39.86874979179853	34.61501049335421	13.372930477364337
6	30.281221892801224	38.06809020953396	14.30174222992105	17.348945667743763
7	24.894633398847397	34.06542523068723	22.66864319264466	18.371298177820712
8	27.399247143475797	34.714347579866086	19.605716379626237	18.28068889703188
9	25.01855491522036	14.37749425363936	22.6563842899497	37.94756654119058
10	16.78303740964056	28.329991005696392	33.30823811585996	21.57873346880309
11	31.786401945434555	23.557946633798593	23.958559578933343	20.697091841833508
12	20.23771611312835	28.40607615177054	31.73563409840434	19.620573636696758
13	30.81475065791665	22.097005230020986	27.500183217295714	19.58806089476665
14	20.26723075385589	23.766347979612913	29.69832439455012	26.26809687198108
15	22.88264099403711	29.828641860155237	26.834005130084282	20.454712015723373
16	20.12145641093974	29.094906559179183	28.773243612378828	22.01039341750225
17	20.288017588860388	28.131583330557312	29.039808121523038	22.540590959059262
18	21.453546087477932	25.958892701289184	31.29771144941537	21.289849761817514
19	22.089476664778974	25.484593091042342	32.87797728105533	19.547952963123354
20	22.283886871647958	25.45761018021919	32.76191745228022	19.496585495852628
21	21.250474699357074	26.003930843798926	33.23555081781539	19.510043639028616
22	20.04430527332689	26.542656317665482	33.03154668709817	20.381491721909455
23	20.41227222758919	26.91815183716979	33.429494653386186	19.240081281854827
24	20.282487757753422	26.891435424231318	32.17302375162397	20.653053066391287
25	20.272960458376364	27.25833638695493	32.73440154568773	19.734301608980978
26	20.163563076718077	27.5037809387388	32.64186015523502	19.690795829308104
27	19.61857490256171	27.737499583597053	32.47389986341984	20.1700256504214
28	19.654818614877247	27.618375029148208	32.45744361904128	20.269362736933275
29	19.774409540624273	27.575868616542852	32.23331889803125	20.416402944801625
30	19.7798061227889	28.05942902828209	32.96205736366968	19.198707485259337
31	20.147106832339517	27.682134648056234	32.7888337386322	19.381924780972053
32	19.922915486858322	27.659815450214865	32.30820480362437	20.109064259302443
33	19.734901229221492	27.629168193477465	32.45624437856024	20.179686198740796
34	19.078450314800627	27.917785402578367	33.02701622305873	19.976748059562276
35	18.968986308671177	29.238415670075618	32.15010493354209	19.642493087711117
36	20.014457510243513	27.833838568906362	32.09347413304907	20.05822978780106
37	19.49691861820847	28.1211232885839	32.07568539924714	20.30627269396049
38	19.422365834971185	27.35261001365802	32.672107665145404	20.55291648622539
39	19.317099170525335	27.051067657150472	33.17878676838003	20.45304640394417
40	18.57543555748026	27.45894266964256	32.86725074119724	21.098371031679935
41	19.371464738998633	26.783637029881074	32.696758719477664	21.148139511642626
42	18.273493454145708	28.04330590625937	32.45977547553217	21.22342516406276
43	18.425930244178687	27.87168126852993	31.8704820280489	21.831906459242482
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	466.0
1	692.5
2	919.0
3	1810.5
4	2702.0
5	2702.0
6	4004.0
7	5306.0
8	5727.0
9	6148.0
10	8741.5
11	11335.0
12	11335.0
13	19134.0
14	26933.0
15	38780.0
16	50627.0
17	48092.5
18	45558.0
19	45558.0
20	48889.0
21	52220.0
22	42321.0
23	32422.0
24	34422.5
25	36423.0
26	36423.0
27	39021.0
28	41619.0
29	44341.5
30	47064.0
31	50694.0
32	54324.0
33	54324.0
34	57911.0
35	61498.0
36	64973.0
37	68448.0
38	70638.5
39	72829.0
40	72829.0
41	74613.0
42	76397.0
43	80030.5
44	83664.0
45	88365.0
46	93066.0
47	93066.0
48	110004.5
49	126943.0
50	120596.0
51	114249.0
52	106336.0
53	98423.0
54	98423.0
55	89035.5
56	79648.0
57	69224.0
58	58800.0
59	51791.5
60	44783.0
61	44783.0
62	39185.5
63	33588.0
64	29039.0
65	24490.0
66	20919.5
67	17349.0
68	17349.0
69	14762.5
70	12176.0
71	10330.0
72	8484.0
73	6863.5
74	5243.0
75	5243.0
76	4254.0
77	3265.0
78	2491.0
79	1717.0
80	1329.5
81	942.0
82	942.0
83	692.0
84	442.0
85	331.5
86	221.0
87	161.5
88	102.0
89	102.0
90	91.5
91	81.0
92	49.5
93	18.0
94	16.0
95	14.0
96	14.0
97	7.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1500950.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.26351378628806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.58389832500356	52.664907194568464
2	9.376486266755467	11.676259638741396
3	2.5935964703961454	4.844592887717354
4	1.0977214205633867	2.7339197121100858
5	0.5808667819722183	1.8083403443662
6	0.3635775136197481	1.3582568118988514
7	0.24998746696482513	1.0895568667034534
8	0.18198295544310025	0.9064718604080735
9	0.13483036926597775	0.7555511300042265
>10	0.7515290951426813	8.562309265450356
>50	0.05273619538617925	2.2529057673776642
>100	0.02807276404898532	3.265682212711373
>500	0.002464332615225369	1.095747511953144
>1k	0.0018214632373404905	2.0314525105927057
>5k	1.071448963141465E-4	0.6274280273923483
>10k+	3.2143468894243945E-4	4.3266182580043315
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23500	1.5656750724541126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22981	1.5310969719177854	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18374	1.2241580332456112	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9405	0.6266031513374862	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2851	0.18994636730070955	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2593	0.17275725373929846	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2527	0.1683600386421933	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2313	0.15410240181218562	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2219	0.14783970152236917	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2097	0.13971151603984144	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2032	0.1353809254139045	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1956	0.13031746560511676	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1672	0.11139611579333089	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1579	0.1052000399746827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.66244711682601E-5	0.0
11	0.0	0.0	0.0	3.9974682700956064E-4	0.0
12	0.0	0.0	0.0	5.996202405143409E-4	0.0
13	0.0	0.0	0.0	6.662447116826011E-4	0.0
14	0.0	0.0	0.0	9.993670675239015E-4	0.0
15	0.0	0.0	0.0	0.0011992404810286818	0.0
16	0.0	0.0	0.0	0.001998734135047803	0.0
17	0.0	0.0	0.0	0.0035977214430860456	0.0
18	0.0	0.0	0.0	0.004197341683600387	0.0
19	0.0	0.0	0.0	0.00632932476098471	0.0
20	0.0	0.0	0.0	0.010793164329258137	0.0
21	0.0	0.0	0.0	0.01972084346580499	0.0
22	0.0	0.0	0.0	0.031047003564409207	0.0
23	0.0	0.0	0.0	0.03904194010460042	0.0
24	0.0	0.0	0.0	0.05103434491488724	0.0
25	6.66244711682601E-5	0.0	0.0	0.06009527299377061	0.0
26	6.66244711682601E-5	0.0	0.0	0.07095506179419701	0.0
27	6.66244711682601E-5	0.0	0.0	0.1345814317598854	0.0
28	6.66244711682601E-5	0.0	0.0	0.2529731170258836	0.0
29	6.66244711682601E-5	0.0	0.0	0.4081415103767614	0.0
30	6.66244711682601E-5	0.0	0.0	0.6022852193610713	0.0
31	6.66244711682601E-5	0.0	0.0	0.859122555714714	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5190	0.0	30.29865	1
AGTCGTG	230	0.0	19.304348	20
GTATCAA	8420	0.0	18.653801	2
TACGCGC	50	0.007035862	18.499998	17
GAGTCGT	250	0.0	17.76	19
TGAGTCG	255	0.0	17.411764	18
TTGGACC	440	0.0	16.397728	4
TATACCG	105	9.34683E-6	15.857142	5
GTATTGG	375	0.0	15.786668	1
GGTGCTC	350	0.0	15.328571	8
CTAGCAC	145	5.3483745E-8	15.310345	3
TATACGT	85	5.3655106E-4	15.235293	4
GTGCTCG	300	0.0	14.8	9
ATACGTC	75	0.0041051838	14.8	5
TATACTG	300	0.0	14.8	5
ACGGTGT	100	1.093718E-4	14.799999	29
GGACCCT	520	0.0	14.586538	6
ATTGGAC	395	0.0	14.518987	3
AGACGCG	90	8.276096E-4	14.388887	14
ACCGTCC	90	8.276096E-4	14.388887	8
>>END_MODULE