FastQCFastQC Report
Wed 25 May 2016
SRR2088680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538993
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT253191.645166677171371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT232031.5076741739566069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191061.241461137250137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90640.588956544961543No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG74460.4838228633918413No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG70010.4549078520824981No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA65240.42391355906102235No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60960.3961031661612496No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG58130.3777145185195774No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52570.34158699877127446No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA39030.2536073913266662No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34110.22163843500262836No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA33660.21871444509494195No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33180.21559552252674313No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27000.17543939446118337No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG26540.17245042699999286No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.16647249207761178No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23640.15360693648379167No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.1493184179525183No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC22610.14691424847286505No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22380.14541976474226978No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22270.1447050116537242No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT22080.14347043813714552No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA22070.14340546058364137No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG21740.1412612013180047No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20190.13118968052486268No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT19790.128590578384697No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT19270.12521174560248163No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG17720.11514022480933962No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG17210.11182636958062836No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA16350.10623829997927217No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG16120.1047438162486769No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT15930.1035092427320982No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT15830.10285946719705678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65450.026.7960261
TTAGACG502.7026792E-422.215
TAGACGT603.727342E-521.58333416
TATACCG705.1037787E-621.1428575
GTATTGG6500.018.7846161
ATTGGAC6500.018.5000023
CGACGAG701.21953824E-418.524
TACACCG1205.176844E-918.55
TATACTG1552.1827873E-1117.9032255
TGGACCC9550.016.8534035
GTATCAA104200.016.8488482
TTGGACC9300.016.5107524
TAATACT2250.016.4444454
ATACGGA904.447531E-516.44444529
ACCGTGA1151.2427881E-616.0869568
GTTCTAA1403.4751793E-815.8571421
GGACCCT9850.015.776656
ATACTGG1659.749783E-1015.696976
TATTGGA7800.015.6538472
CTAATAC1902.7284841E-1115.5789483
Produced by FastQC (version 0.11.5)