##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1538993
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.642646197870945	31.0	31.0	34.0	30.0	34.0
2	31.839389133023996	31.0	31.0	34.0	30.0	34.0
3	31.896937153060474	31.0	31.0	34.0	30.0	34.0
4	35.58795589063758	37.0	35.0	37.0	33.0	37.0
5	35.50110169441966	37.0	35.0	37.0	33.0	37.0
6	35.574347641607204	37.0	35.0	37.0	33.0	37.0
7	35.42154187835812	37.0	35.0	37.0	33.0	37.0
8	35.47700216960051	37.0	35.0	37.0	33.0	37.0
9	37.06435571831711	39.0	37.0	39.0	33.0	39.0
10	36.9109729543929	39.0	37.0	39.0	33.0	39.0
11	36.99503636468782	39.0	37.0	39.0	33.0	39.0
12	36.84114352696861	39.0	37.0	39.0	32.0	39.0
13	36.88012161198914	39.0	37.0	39.0	33.0	39.0
14	37.81854888228861	40.0	37.0	41.0	32.0	41.0
15	37.8265703612687	40.0	37.0	41.0	32.0	41.0
16	37.71964589832442	39.0	37.0	41.0	32.0	41.0
17	37.81104332508335	40.0	37.0	41.0	32.0	41.0
18	37.78279693279956	40.0	37.0	41.0	32.0	41.0
19	37.84466336104193	40.0	37.0	41.0	32.0	41.0
20	37.737933830758166	40.0	37.0	41.0	32.0	41.0
21	37.68339232212232	40.0	37.0	41.0	32.0	41.0
22	37.56593694708163	39.0	36.0	41.0	32.0	41.0
23	37.36400555428127	39.0	36.0	41.0	31.0	41.0
24	37.202720220299895	39.0	36.0	41.0	31.0	41.0
25	37.37723368462365	39.0	36.0	41.0	31.0	41.0
26	37.239253199982066	39.0	36.0	41.0	31.0	41.0
27	37.13249442979922	39.0	36.0	41.0	31.0	41.0
28	36.94224405179231	39.0	35.0	41.0	31.0	41.0
29	36.81319213277773	39.0	35.0	41.0	30.0	41.0
30	36.574970126569774	39.0	35.0	40.0	30.0	41.0
31	36.36206402498257	38.0	35.0	40.0	30.0	41.0
32	36.18362331732503	38.0	35.0	40.0	30.0	41.0
33	36.00160884422476	38.0	35.0	40.0	30.0	41.0
34	35.808431877208015	38.0	35.0	40.0	29.0	41.0
35	35.634424588026064	38.0	35.0	40.0	27.0	41.0
36	35.418173441984464	38.0	34.0	40.0	26.0	41.0
37	35.30123009006539	38.0	34.0	40.0	25.0	41.0
38	35.091939339555154	38.0	34.0	40.0	25.0	41.0
39	34.95097443588112	38.0	34.0	40.0	24.0	41.0
40	34.72114947891251	38.0	33.0	40.0	23.0	41.0
41	34.53245791241416	38.0	33.0	40.0	22.0	41.0
42	34.19965652865218	38.0	33.0	40.0	20.0	41.0
43	33.821655459121644	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	0.0
14	1.0
15	8.0
16	21.0
17	45.0
18	87.0
19	259.0
20	511.0
21	1034.0
22	2049.0
23	3569.0
24	5913.0
25	9353.0
26	13204.0
27	18881.0
28	25601.0
29	33664.0
30	42127.0
31	51619.0
32	63871.0
33	78637.0
34	97144.0
35	118555.0
36	154390.0
37	218196.0
38	276872.0
39	323379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34508733957854	21.345451213878167	14.971283170228844	22.338178276314448
2	18.92588205404443	23.861641995772562	35.37365017254789	21.838825777635115
3	19.82543130475577	24.02447574485394	34.02042764327064	22.129665307119655
4	13.414745876037124	16.075186826710713	35.81653717723213	34.693530120020036
5	11.977312437418494	38.88594684966078	34.939340204926204	14.197400507994512
6	31.91203598716823	36.868848656231705	12.722799908771515	18.49631544782855
7	25.136696528184338	34.25285235215495	20.630698125332604	19.97975299432811
8	27.40109929025018	34.12673092080341	18.620422575021458	19.85174721392495
9	23.862876569289142	14.463353634486968	22.6731375646283	39.000632231595596
10	16.31599363999706	27.993694578207958	33.132249464422515	22.558062317372464
11	32.2550524921166	22.149548438491923	24.282891475139913	21.312507594251564
12	21.31959014758352	27.72000912284851	30.99741194404393	19.96298878552404
13	32.757783823578144	21.028750618098975	26.713181931301833	19.500283627021044
14	19.977413802401962	23.698158471156137	28.691098659967913	27.633329066473987
15	21.73739581661515	29.816639841766662	26.78043369917862	21.66553064243957
16	20.828359843092205	28.779143244966026	27.555291024715512	22.837205887226258
17	21.11257166211932	26.786346656547494	27.161267140266393	24.939814541066788
18	23.290554278024654	25.05326534948502	30.63854091604055	21.017639456449768
19	22.442792137456117	24.183670750939086	32.25427276147455	21.119264350130248
20	23.909270542491097	23.32447256095382	32.199561661424056	20.566695235131025
21	22.12362239464377	24.653913305648565	33.312302265182495	19.91016203452517
22	20.056101619695475	24.914928138074703	32.69196156187844	22.33700868035137
23	21.78034597948139	25.23585227483166	33.82289588061804	19.160905865068912
24	21.508544873173562	25.098359771616895	30.78701462579752	22.606080729412025
25	21.261175326983295	26.00018323670088	32.07629924242671	20.66234219388912
26	20.98502072459069	26.763669490374546	31.853881076782027	20.397428708252736
27	19.20885929955497	26.94450202177658	31.605861755056715	22.240776923611737
28	20.24973472913782	26.031307484829362	31.57473750692823	22.144220279104584
29	20.39314018972146	25.949435767414148	30.776423284576342	22.88100075828805
30	20.839471004741412	26.273348871632297	33.112951131031785	19.77422899259451
31	22.009586788244	25.926693623687697	32.40320131410604	19.66051827396226
32	21.517251865343116	25.901612288035096	31.038932600733077	21.542203245888707
33	20.758054130200723	25.742157371735935	32.00358936005557	21.496199138007775
34	19.441478941099795	26.736638828116828	32.850441814875055	20.971440415908322
35	18.928741066398615	29.45075123798484	31.28454775297873	20.335959942637814
36	22.10010052027527	26.036180801342173	31.11326692194182	20.75045175644074
37	20.938951639156254	27.412145474345888	30.954591736284698	20.69431115021316
38	20.710880426356717	25.28874400338403	32.620616208130905	21.379759362128354
39	20.070331703912885	25.027794148511397	33.48111394918625	21.420760198389466
40	18.518862658894484	25.755932613078812	32.914119817309114	22.811084910717593
41	20.645123142210522	24.824609338703947	32.8276346935951	21.702632825490433
42	19.11236763260132	27.349507112767895	31.983641251129797	21.55448400350099
43	19.426339171133332	27.671146002613394	30.73106895223045	22.17144587402282
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	588.0
1	498.5
2	409.0
3	842.5
4	1276.0
5	1276.0
6	2108.0
7	2940.0
8	3281.0
9	3622.0
10	5785.0
11	7948.0
12	7948.0
13	15155.5
14	22363.0
15	35989.0
16	49615.0
17	45956.5
18	42298.0
19	42298.0
20	44413.0
21	46528.0
22	35927.0
23	25326.0
24	27263.5
25	29201.0
26	29201.0
27	31167.0
28	33133.0
29	34510.0
30	35887.0
31	39085.5
32	42284.0
33	42284.0
34	46429.5
35	50575.0
36	55092.5
37	59610.0
38	63014.5
39	66419.0
40	66419.0
41	69159.0
42	71899.0
43	76059.0
44	80219.0
45	91018.5
46	101818.0
47	101818.0
48	148312.5
49	194807.0
50	175747.0
51	156687.0
52	138657.0
53	120627.0
54	120627.0
55	104422.5
56	88218.0
57	72115.0
58	56012.0
59	49398.5
60	42785.0
61	42785.0
62	37580.5
63	32376.0
64	28278.5
65	24181.0
66	20680.0
67	17179.0
68	17179.0
69	14591.0
70	12003.0
71	10122.0
72	8241.0
73	6690.0
74	5139.0
75	5139.0
76	4155.5
77	3172.0
78	2438.5
79	1705.0
80	1337.0
81	969.0
82	969.0
83	724.0
84	479.0
85	364.0
86	249.0
87	173.5
88	98.0
89	98.0
90	79.0
91	60.0
92	43.5
93	27.0
94	21.5
95	16.0
96	16.0
97	10.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1538993.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.04532059917758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8416862941578	40.957934289684445
2	10.331178788081901	10.340543092339633
3	3.1006668152226133	4.655215945171398
4	1.386562087453846	2.7756377678917055
5	0.7389907390746597	1.8491514228407266
6	0.4883750230270015	1.4664530760010213
7	0.32865679645172446	1.1513414321867639
8	0.2515537642724246	1.0071271024754744
9	0.2039799169681274	0.9187416306417201
>10	1.158448703892163	10.869647273804912
>50	0.09921772206742181	3.3765005417591065
>100	0.05730034781025661	5.540074417656564
>500	0.005846942411700082	2.0975086116050967
>1k	0.006236738572480088	5.527377686110911
>5k	9.09524375153346E-4	3.0692409082175085
>10k+	3.897961607800055E-4	4.397504801612968
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25319	1.645166677171371	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23203	1.5076741739566069	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19106	1.241461137250137	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9064	0.588956544961543	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7446	0.4838228633918413	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7001	0.4549078520824981	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6524	0.42391355906102235	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6096	0.3961031661612496	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5813	0.3777145185195774	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5257	0.34158699877127446	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3903	0.2536073913266662	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3411	0.22163843500262836	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3366	0.21871444509494195	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3318	0.21559552252674313	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2700	0.17543939446118337	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2654	0.17245042699999286	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2562	0.16647249207761178	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2364	0.15360693648379167	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2298	0.1493184179525183	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2261	0.14691424847286505	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2238	0.14541976474226978	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2227	0.1447050116537242	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2208	0.14347043813714552	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2207	0.14340546058364137	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2174	0.1412612013180047	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2019	0.13118968052486268	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1979	0.128590578384697	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1927	0.12521174560248163	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1772	0.11514022480933962	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1721	0.11182636958062836	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1635	0.10623829997927217	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1612	0.1047438162486769	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1593	0.1035092427320982	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1583	0.10285946719705678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.4977553504142E-5	0.0	0.0	0.0	0.0
7	6.4977553504142E-5	0.0	0.0	0.0	0.0
8	6.4977553504142E-5	0.0	0.0	0.0	0.0
9	6.4977553504142E-5	0.0	0.0	6.4977553504142E-5	0.0
10	6.4977553504142E-5	0.0	0.0	2.59910214016568E-4	0.0
11	6.4977553504142E-5	0.0	0.0	5.84797981537278E-4	0.0
12	6.4977553504142E-5	0.0	0.0	9.096857490579879E-4	0.0
13	6.4977553504142E-5	0.0	0.0	0.001169595963074556	0.0
14	6.4977553504142E-5	0.0	0.0	0.0013645286235869818	0.0
15	6.4977553504142E-5	0.0	0.0	0.0017543939446118338	0.0
16	6.4977553504142E-5	0.0	0.0	0.0030539450146946736	0.0
17	6.4977553504142E-5	0.0	0.0	0.004743361405802365	0.0
18	6.4977553504142E-5	0.0	0.0	0.0055880696013562116	0.0
19	6.4977553504142E-5	0.0	0.0	0.006757665564430767	0.0
20	6.4977553504142E-5	0.0	0.0	0.011241116756216566	0.0
21	6.4977553504142E-5	0.0	0.0	0.021507570209871	0.0
22	6.4977553504142E-5	0.0	0.0	0.03677729528334437	0.0
23	6.4977553504142E-5	0.0	0.0	0.045224377238882826	0.0
24	6.4977553504142E-5	0.0	0.0	0.05588069601356212	0.0
25	6.4977553504142E-5	0.0	0.0	0.06296324934551359	0.0
26	6.4977553504142E-5	0.0	0.0	0.07569884983232543	0.0
27	6.4977553504142E-5	0.0	0.0	0.14671931581235262	0.0
28	6.4977553504142E-5	0.0	0.0	0.29454325003427567	0.0
29	6.4977553504142E-5	0.0	0.0	0.49012568608174306	0.0
30	6.4977553504142E-5	0.0	0.0	0.7961699630862519	0.0
31	6.4977553504142E-5	0.0	0.0	1.131129251400104	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6545	0.0	26.796026	1
TTAGACG	50	2.7026792E-4	22.2	15
TAGACGT	60	3.727342E-5	21.583334	16
TATACCG	70	5.1037787E-6	21.142857	5
GTATTGG	650	0.0	18.784616	1
ATTGGAC	650	0.0	18.500002	3
CGACGAG	70	1.21953824E-4	18.5	24
TACACCG	120	5.176844E-9	18.5	5
TATACTG	155	2.1827873E-11	17.903225	5
TGGACCC	955	0.0	16.853403	5
GTATCAA	10420	0.0	16.848848	2
TTGGACC	930	0.0	16.510752	4
TAATACT	225	0.0	16.444445	4
ATACGGA	90	4.447531E-5	16.444445	29
ACCGTGA	115	1.2427881E-6	16.086956	8
GTTCTAA	140	3.4751793E-8	15.857142	1
GGACCCT	985	0.0	15.77665	6
ATACTGG	165	9.749783E-10	15.69697	6
TATTGGA	780	0.0	15.653847	2
CTAATAC	190	2.7284841E-11	15.578948	3
>>END_MODULE