FastQCFastQC Report
Wed 25 May 2016
SRR2088679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105345
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT234361.1131667256435405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT214891.0206878207609678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168110.7984914586445452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85610.40663169219296597No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59810.2840864561390176No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG56780.269694515625705No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA52700.25031526899391787No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50680.2407206419850428No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG45910.21806402276111514No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40240.1911325697213521No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32940.15645891765957598No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC31340.1488592130980908No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28760.13660468949269597No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27260.12947996646630364No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.11831790039162227No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.10620587124675529No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22070.1048284247949861No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG21800.10354597465023548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.024.421
TGTTACG705.1052484E-621.14285916
ATTGGAC6450.018.6434123
CGCGAAT500.007036778618.535
TATACCG1451.546141E-1017.8620685
GACCGTT1156.412483E-817.6956527
CCGTTTA1156.412483E-817.69565227
CAATGCG953.6092715E-617.52631619
GTATTGG6700.017.3955231
TATACTG2650.016.7547175
AAGACCG1801.0913936E-1116.4444455
GTATCAA97850.015.5600412
TTGGACC10700.014.869164
CGACGAG1508.110692E-814.824
CGTTATT1001.09405E-414.810
GGACCGT1508.110692E-814.86
TACCGAC3250.014.87
GTATTAG3550.014.5915491
ACGTTTA2059.276846E-1114.43902426
ACCGTTA1304.4479E-614.2307698
Produced by FastQC (version 0.11.5)