##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2105345
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.618102971246994	31.0	31.0	34.0	30.0	34.0
2	31.833549845749747	31.0	31.0	34.0	30.0	34.0
3	31.909881753346838	31.0	31.0	34.0	30.0	34.0
4	35.62583757056444	37.0	35.0	37.0	33.0	37.0
5	35.50468165550064	37.0	35.0	37.0	33.0	37.0
6	35.54964150768639	37.0	35.0	37.0	33.0	37.0
7	35.410630086755376	37.0	35.0	37.0	33.0	37.0
8	35.43593567800052	37.0	35.0	37.0	33.0	37.0
9	37.039989645402535	39.0	37.0	39.0	33.0	39.0
10	36.85941829011397	39.0	37.0	39.0	32.0	39.0
11	36.94916890105897	39.0	37.0	39.0	33.0	39.0
12	36.805229071719836	39.0	37.0	39.0	32.0	39.0
13	36.8400960412664	39.0	37.0	39.0	32.0	39.0
14	37.82731476313858	40.0	37.0	41.0	32.0	41.0
15	37.84189812121054	40.0	37.0	41.0	32.0	41.0
16	37.73987066252799	40.0	37.0	41.0	32.0	41.0
17	37.764641899546156	39.0	37.0	41.0	32.0	41.0
18	37.72455535791046	39.0	37.0	41.0	32.0	41.0
19	37.75990158382593	40.0	37.0	41.0	32.0	41.0
20	37.64157750867435	39.0	37.0	41.0	32.0	41.0
21	37.572295277021105	39.0	37.0	41.0	32.0	41.0
22	37.448776328820216	39.0	36.0	41.0	32.0	41.0
23	37.26391731521437	39.0	36.0	41.0	31.0	41.0
24	37.110427032149126	39.0	36.0	41.0	31.0	41.0
25	37.30239271948303	39.0	36.0	41.0	31.0	41.0
26	37.18309730709219	39.0	36.0	41.0	31.0	41.0
27	37.075588086513136	39.0	36.0	41.0	31.0	41.0
28	36.901391458407055	39.0	35.0	41.0	30.0	41.0
29	36.790444321477004	39.0	35.0	41.0	30.0	41.0
30	36.596344542105925	39.0	35.0	40.0	30.0	41.0
31	36.44022998605929	39.0	35.0	40.0	30.0	41.0
32	36.26544010601588	38.0	35.0	40.0	30.0	41.0
33	36.1219652835996	38.0	35.0	40.0	30.0	41.0
34	35.94326203068856	38.0	35.0	40.0	29.0	41.0
35	35.812576086104656	38.0	35.0	40.0	28.0	41.0
36	35.62010501841741	38.0	35.0	40.0	27.0	41.0
37	35.545378548408934	38.0	34.0	40.0	26.0	41.0
38	35.367396317468156	38.0	34.0	40.0	26.0	41.0
39	35.267153364412955	38.0	34.0	40.0	25.0	41.0
40	35.10099104897297	38.0	34.0	40.0	24.0	41.0
41	34.960944168295455	38.0	34.0	40.0	24.0	41.0
42	34.70490252191446	38.0	33.0	40.0	23.0	41.0
43	34.3473231228136	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	3.0
15	13.0
16	24.0
17	69.0
18	151.0
19	327.0
20	719.0
21	1505.0
22	2844.0
23	4814.0
24	7965.0
25	12083.0
26	17529.0
27	24648.0
28	33536.0
29	43872.0
30	56062.0
31	69486.0
32	86242.0
33	106646.0
34	131783.0
35	163555.0
36	210309.0
37	289812.0
38	389807.0
39	451539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28097770199183	21.06039627709473	14.02093243625154	23.637693584661896
2	19.040157313884425	23.193965834578183	34.741669417601386	23.024207433936006
3	19.616072425184473	24.041712878411854	32.888908943664816	23.453305752738864
4	13.971391862141358	16.337037397671168	35.863718297951166	33.827852442236306
5	12.860552546019774	38.21064956099832	34.88953116947579	14.039266723506124
6	32.617172007438214	36.943018840142585	13.68141563496719	16.758393517452006
7	26.09486806200409	33.086406265956406	21.337832991742445	19.480892680297053
8	27.147902125304878	34.17976626158658	19.03968233234933	19.63264928075921
9	24.651161686089456	14.519568051791987	21.733730101242312	39.09554016087625
10	16.413176937746545	27.716407524657477	33.54766083468505	22.322754702910924
11	33.314397402800964	22.45536954750884	23.460620468379293	20.769612581310902
12	21.02230275798028	27.055613213036345	30.87484474041072	21.047239288572655
13	31.634720200252215	21.080250505261606	26.045707473121983	21.239321821364193
14	20.56598799721661	23.02805478437026	27.649007644827805	28.756949573585327
15	22.76315758224899	29.798584080043888	25.089237155905565	22.349021181801557
16	22.09519104944795	28.314266782878818	26.42336529167429	23.167176875998948
17	21.7884004759315	27.086297020203336	26.831469426626043	24.29383307723912
18	23.173351635955154	25.88297879919918	29.149854299414113	21.793815265431558
19	22.79156147804754	25.153169670529056	30.39202600998886	21.66324284143454
20	23.842030641058827	24.771617003388993	29.946493330071792	21.439859025480384
21	22.643842220633672	25.543699488682375	30.601730357732343	21.210727932951606
22	21.350609995036443	25.89836820093619	30.19562114522798	22.55540065879939
23	22.414188648416292	25.99402948210388	30.82611163491019	20.76567023456963
24	22.216263842743114	26.01421619734533	28.828481792770305	22.941038167141254
25	22.248705081589954	26.345848305147136	29.867456402632346	21.53799021063056
26	21.864112532625292	27.069672666475093	29.681501131643508	21.384713669256108
27	20.845609626925754	27.065872814194346	29.484193801965947	22.60432375691395
28	21.408415247857242	26.672968088365568	29.458639795377955	22.45997686839924
29	21.55081471207807	26.55873502917574	29.017524443737248	22.87292581500894
30	21.813337006523874	26.82424970729263	30.37435669688341	20.98805658930009
31	22.610641011330685	26.494470027477686	30.068231097516083	20.82665786367555
32	22.11195789763673	26.412963196055756	29.24964791993711	22.225430986370405
33	21.6548356682634	26.42175035445497	29.859904196224374	22.06350978105726
34	20.882753182970013	26.79104849798964	30.496949431090865	21.829248887949483
35	20.52342965167229	28.541925432648807	29.49545086434765	21.439194051331253
36	22.502725206557596	26.412773203441713	29.437455618912818	21.647045971087874
37	21.68319206590844	27.01200990811482	29.472651750663193	21.832146275313548
38	21.45410847153317	25.82151618855817	30.672977587996265	22.051397751912393
39	21.034604779739187	25.49069154936602	31.44073774132031	22.033965929574485
40	19.98793546900864	25.864217028563015	31.20961172634414	22.938235776084205
41	21.199328376109378	25.052806072163946	31.202581999624762	22.54528355210191
42	20.068919820741968	26.802495552985377	30.606527671236783	22.522056955035875
43	20.255207578805372	26.70246444169483	30.07915567282322	22.963172306676576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	636.0
1	621.5
2	607.0
3	1072.0
4	1537.0
5	1537.0
6	2360.5
7	3184.0
8	3521.0
9	3858.0
10	5965.0
11	8072.0
12	8072.0
13	15278.5
14	22485.0
15	35358.0
16	48231.0
17	44914.5
18	41598.0
19	41598.0
20	45200.0
21	48802.0
22	40047.0
23	31292.0
24	35219.0
25	39146.0
26	39146.0
27	43009.5
28	46873.0
29	49796.5
30	52720.0
31	58126.0
32	63532.0
33	63532.0
34	70323.5
35	77115.0
36	84063.5
37	91012.0
38	96063.0
39	101114.0
40	101114.0
41	106101.0
42	111088.0
43	117612.5
44	124137.0
45	132947.0
46	141757.0
47	141757.0
48	181025.0
49	220293.0
50	205431.5
51	190570.0
52	173280.0
53	155990.0
54	155990.0
55	139737.0
56	123484.0
57	107692.5
58	91901.0
59	82581.0
60	73261.0
61	73261.0
62	65280.5
63	57300.0
64	50320.0
65	43340.0
66	36921.0
67	30502.0
68	30502.0
69	26275.0
70	22048.0
71	18596.0
72	15144.0
73	12379.0
74	9614.0
75	9614.0
76	7712.0
77	5810.0
78	4516.0
79	3222.0
80	2595.0
81	1968.0
82	1968.0
83	1491.0
84	1014.0
85	783.0
86	552.0
87	425.0
88	298.0
89	298.0
90	219.5
91	141.0
92	101.0
93	61.0
94	44.5
95	28.0
96	28.0
97	17.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2105345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.152468794486666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4457666725606	38.558030984326166
2	11.755313675700238	11.556053772285166
3	3.8456996867907143	5.670769015438433
4	1.7172812332238152	3.3763444890956467
5	0.9643510447704546	2.370011731750519
6	0.6260632262217746	1.8463531914144848
7	0.4120628068711647	1.4177732979272457
8	0.2961316381473349	1.1644480882477677
9	0.2481360706464597	1.0976850422312983
>10	1.4933407615726884	13.563035660797826
>50	0.12307960708725588	4.1625041230386985
>100	0.06348518929613672	5.738263141912922
>500	0.004644193555658503	1.6456147183146994
>1k	0.0038701612963820855	3.4438873924653146
>5k	4.837701620477607E-4	1.453246671163211
>10k+	2.902620972286564E-4	2.9359786795906797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23436	1.1131667256435405	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21489	1.0206878207609678	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16811	0.7984914586445452	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8561	0.40663169219296597	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5981	0.2840864561390176	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5678	0.269694515625705	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5270	0.25031526899391787	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5068	0.2407206419850428	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4591	0.21806402276111514	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4024	0.1911325697213521	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3294	0.15645891765957598	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3134	0.1488592130980908	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2876	0.13660468949269597	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2726	0.12947996646630364	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2491	0.11831790039162227	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2236	0.10620587124675529	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2207	0.1048284247949861	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2180	0.10354597465023548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.7498153509282324E-5	0.0
2	0.0	0.0	0.0	4.7498153509282324E-5	0.0
3	0.0	0.0	0.0	4.7498153509282324E-5	0.0
4	0.0	0.0	0.0	4.7498153509282324E-5	0.0
5	0.0	0.0	0.0	4.7498153509282324E-5	0.0
6	9.499630701856465E-5	0.0	0.0	4.7498153509282324E-5	0.0
7	9.499630701856465E-5	0.0	0.0	4.7498153509282324E-5	0.0
8	9.499630701856465E-5	0.0	0.0	4.7498153509282324E-5	0.0
9	9.499630701856465E-5	0.0	0.0	9.499630701856465E-5	0.0
10	9.499630701856465E-5	0.0	0.0	9.499630701856465E-5	0.0
11	9.499630701856465E-5	0.0	0.0	1.4249446052784699E-4	0.0
12	9.499630701856465E-5	0.0	0.0	2.8498892105569397E-4	0.0
13	9.499630701856465E-5	0.0	0.0	2.8498892105569397E-4	0.0
14	9.499630701856465E-5	0.0	0.0	3.324870745649763E-4	0.0
15	9.499630701856465E-5	0.0	0.0	5.224796886021056E-4	0.0
16	9.499630701856465E-5	0.0	0.0	0.0010449593772042113	0.0
17	9.499630701856465E-5	0.0	0.0	0.0015199409122970344	0.0
18	9.499630701856465E-5	0.0	0.0	0.0018049298333527284	0.0
19	9.499630701856465E-5	0.0	0.0	0.0023749076754641164	0.0
20	1.4249446052784699E-4	0.0	0.0	0.0037523541272333036	0.0
21	1.4249446052784699E-4	0.0	0.0	0.007219719333410914	0.0
22	1.4249446052784699E-4	0.0	0.0	0.011114567921172065	0.0
23	1.4249446052784699E-4	0.0	0.0	0.013869460824710439	0.0
24	1.899926140371293E-4	0.0	0.0	0.01861927617563867	0.0
25	2.3749076754641163E-4	0.0	0.0	0.022894109991474083	0.0
26	2.3749076754641163E-4	0.0	0.0	0.030683807166996382	0.0
27	2.3749076754641163E-4	0.0	0.0	0.0930013845711748	0.0
28	2.3749076754641163E-4	0.0	0.0	0.21136678311630636	0.0
29	2.3749076754641163E-4	0.0	0.0	0.36492831341181614	0.0
30	2.3749076754641163E-4	0.0	0.0	0.576722579909706	0.0
31	2.3749076754641163E-4	0.0	0.0	0.8250904246097432	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6250	0.0	24.42	1
TGTTACG	70	5.1052484E-6	21.142859	16
ATTGGAC	645	0.0	18.643412	3
CGCGAAT	50	0.0070367786	18.5	35
TATACCG	145	1.546141E-10	17.862068	5
GACCGTT	115	6.412483E-8	17.695652	7
CCGTTTA	115	6.412483E-8	17.695652	27
CAATGCG	95	3.6092715E-6	17.526316	19
GTATTGG	670	0.0	17.395523	1
TATACTG	265	0.0	16.754717	5
AAGACCG	180	1.0913936E-11	16.444445	5
GTATCAA	9785	0.0	15.560041	2
TTGGACC	1070	0.0	14.86916	4
CGACGAG	150	8.110692E-8	14.8	24
CGTTATT	100	1.09405E-4	14.8	10
GGACCGT	150	8.110692E-8	14.8	6
TACCGAC	325	0.0	14.8	7
GTATTAG	355	0.0	14.591549	1
ACGTTTA	205	9.276846E-11	14.439024	26
ACCGTTA	130	4.4479E-6	14.230769	8
>>END_MODULE