FastQCFastQC Report
Wed 25 May 2016
SRR2088678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928391
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210271.090390901015406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192951.00057509083998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158360.8212027540058007No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.42029858052645963No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG63420.32887521254766283No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG53250.27613694525643395No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA49370.2560165443626318No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48240.2501567368858286No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46350.2403558199556003No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG42520.22049470257847087No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC29870.15489597286027576No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA29260.15173271395686871No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28300.14675447043675272No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27470.14245036405998576No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG23450.12160396931950004No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.11859628052609662No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22450.11641829898604587No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.1058395315057994No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.10220956227238148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61150.024.0515121
GTATTGG7900.018.7341771
ATTGGAC8800.017.6590923
TTGGACC10800.017.3009264
GAACCGT650.001580458617.0769256
GGACCCT11050.016.4072386
GTATTAG4200.016.2976191
TGGACCC12050.015.96680455
GACCCTC11750.015.9021287
CTAATAC4850.015.2577323
TGCGACG855.366494E-415.23529322
TATACTG3050.015.1639345
CCGTATA1353.9759107E-715.0740752
GTATCAA97600.015.0502042
TATTGGA10300.014.9077662
GTCTAGG1001.0939744E-414.7999991
CGCGATA750.004105748614.79999914
TATCGCC750.004105748614.79999933
TATACGT1406.0004095E-714.5357154
AATACTG4900.014.3469385
Produced by FastQC (version 0.11.5)