##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1928391
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65503676380983	31.0	31.0	34.0	30.0	34.0
2	31.863065114906675	31.0	31.0	34.0	30.0	34.0
3	31.942267932177653	31.0	31.0	34.0	30.0	34.0
4	35.63790693899733	37.0	35.0	37.0	33.0	37.0
5	35.53059934422013	37.0	35.0	37.0	33.0	37.0
6	35.58050467980819	37.0	35.0	37.0	33.0	37.0
7	35.44586134243522	37.0	35.0	37.0	33.0	37.0
8	35.4765221368488	37.0	35.0	37.0	33.0	37.0
9	37.08931072588495	39.0	37.0	39.0	33.0	39.0
10	36.921154475414994	39.0	37.0	39.0	33.0	39.0
11	36.99974849498883	39.0	37.0	39.0	33.0	39.0
12	36.8625833661327	39.0	37.0	39.0	32.0	39.0
13	36.90321776029861	39.0	37.0	39.0	33.0	39.0
14	37.91664086795676	40.0	37.0	41.0	33.0	41.0
15	37.9240766006479	40.0	37.0	41.0	33.0	41.0
16	37.81872400358641	40.0	37.0	41.0	32.0	41.0
17	37.8413558246227	40.0	37.0	41.0	32.0	41.0
18	37.80667043146333	40.0	37.0	41.0	32.0	41.0
19	37.84597366405465	40.0	37.0	41.0	32.0	41.0
20	37.725590920098675	40.0	37.0	41.0	32.0	41.0
21	37.6666920764513	39.0	37.0	41.0	32.0	41.0
22	37.540886677027636	39.0	36.0	41.0	32.0	41.0
23	37.37955476871651	39.0	36.0	41.0	31.0	41.0
24	37.23614557421187	39.0	36.0	41.0	31.0	41.0
25	37.4206195735201	39.0	36.0	41.0	32.0	41.0
26	37.3175932681702	39.0	36.0	41.0	31.0	41.0
27	37.214963148033775	39.0	36.0	41.0	31.0	41.0
28	37.05662907574242	39.0	36.0	41.0	31.0	41.0
29	36.95179037861098	39.0	36.0	41.0	31.0	41.0
30	36.7667993679705	39.0	35.0	41.0	30.0	41.0
31	36.63339748007536	39.0	35.0	40.0	30.0	41.0
32	36.47163256829139	39.0	35.0	40.0	30.0	41.0
33	36.33160650511229	38.0	35.0	40.0	30.0	41.0
34	36.16089942340532	38.0	35.0	40.0	30.0	41.0
35	36.01078878712875	38.0	35.0	40.0	29.0	41.0
36	35.824709304285285	38.0	35.0	40.0	28.0	41.0
37	35.76076998907379	38.0	35.0	40.0	27.0	41.0
38	35.580756184819364	38.0	35.0	40.0	27.0	41.0
39	35.48846992129708	38.0	35.0	40.0	26.0	41.0
40	35.322184142116406	38.0	34.0	40.0	25.0	41.0
41	35.183998473338654	38.0	34.0	40.0	24.0	41.0
42	34.93203504890865	38.0	34.0	40.0	24.0	41.0
43	34.58478182069923	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	2.0
15	12.0
16	15.0
17	45.0
18	113.0
19	269.0
20	560.0
21	1202.0
22	2273.0
23	3964.0
24	6295.0
25	10028.0
26	14656.0
27	21169.0
28	28595.0
29	38115.0
30	49156.0
31	60929.0
32	76214.0
33	95251.0
34	118596.0
35	145875.0
36	190822.0
37	263708.0
38	362777.0
39	437747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.458449038602645	21.203635569757378	13.856992694946202	23.480922696693774
2	18.79338785547122	23.32913812603357	35.145206547842214	22.732267470652996
3	19.67267011721171	24.490209713693957	32.5967607191695	23.24035944992483
4	13.659159371724924	16.274552204402532	36.44203898483243	33.62424943904011
5	12.615646930523944	38.39221402713454	35.210286710527065	13.78185233181445
6	32.36034600866733	37.132303562918516	13.677101791078677	16.83024863733548
7	26.135985907422306	32.8354571246184	21.708097579795798	19.3204593881635
8	26.852749260912333	34.44628190029926	19.28255213802595	19.41841670076245
9	24.479579089510377	14.454589344173458	21.841213737255565	39.2246178290606
10	16.107781046478646	27.612398108060038	33.90717961243337	22.37264123302795
11	32.957735231081244	22.45613052539656	23.733931552263	20.85220269125919
12	20.906496659650458	27.032173454449847	31.163026585376098	20.898303300523597
13	31.358733783760655	21.134147587289092	26.36503696605097	21.142081662899276
14	20.4348599428228	22.92662639475086	27.76330111476355	28.875212547662795
15	22.459345641003303	29.61748939919342	25.642984228820815	22.28018073098246
16	21.813107404048246	28.516830870917776	26.696608727172034	22.973452997861948
17	21.48864001128402	26.990895518595554	27.063287476450576	24.45717699366985
18	22.999225779419216	25.97465970334854	29.4434583028027	21.582656214429544
19	22.66609831719812	25.0029687962659	30.505276160280776	21.825656726255204
20	23.677718885848357	24.673108306354884	30.360855241494072	21.288317566302684
21	22.397273167111855	25.560635783925562	30.944087583897662	21.098003465064917
22	21.15063801894948	25.767440316823713	30.506572577864137	22.57534908636267
23	22.076280173471044	26.208740862200663	31.278926317328796	20.436052646999496
24	22.143071607365933	26.020241745579604	28.985096902028683	22.851589745025773
25	21.956594902174924	26.48135155163035	30.058012094020352	21.504041452174377
26	21.78738647919431	27.08288931031103	29.907731367756853	21.221992842737805
27	20.628026162743968	27.163941337622923	29.63963221151727	22.568400288115846
28	21.173506825120008	26.59787356402306	29.879469464439524	22.3491501464174
29	21.430975357176006	26.449200395562933	29.344308286027054	22.775515961234003
30	21.52130973438478	26.81738298923818	30.784731934550617	20.876575341826424
31	22.311605893203193	26.54451301629182	30.465294642009844	20.678586448495146
32	21.848732959239076	26.445777853142854	29.575381756085772	22.1301074315323
33	21.40188374660533	26.327077859210085	30.294374947819193	21.97666344636539
34	20.688076225205364	26.87406236598283	30.836329354368488	21.601532054443314
35	20.340688169567272	28.757705257906725	29.698748853318648	21.202857719207362
36	22.31497657891994	26.61483070601346	29.514813126591026	21.555379588475574
37	21.503989595471044	27.21725002865083	29.59270189499951	21.686058480878618
38	21.266641464308847	25.84066198193209	30.84120388448193	22.051492669277135
39	20.78297399230758	25.627738358040457	31.641663957153916	21.94762369249805
40	19.80941624390489	26.109746415535025	31.273481363478673	22.80735597708141
41	21.26202621771207	25.16325786627297	31.227277040807593	22.347438875207363
42	19.88035621406655	27.171356846199757	30.616975499263376	22.33131144047032
43	20.276437714135774	26.961077914178194	29.915198733037023	22.84728563864901
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	610.0
1	566.5
2	523.0
3	971.5
4	1420.0
5	1420.0
6	2252.5
7	3085.0
8	3322.0
9	3559.0
10	5570.0
11	7581.0
12	7581.0
13	14236.5
14	20892.0
15	32290.5
16	43689.0
17	41240.0
18	38791.0
19	38791.0
20	41344.5
21	43898.0
22	36064.5
23	28231.0
24	31784.5
25	35338.0
26	35338.0
27	39385.5
28	43433.0
29	46998.0
30	50563.0
31	55710.0
32	60857.0
33	60857.0
34	67187.0
35	73517.0
36	80310.0
37	87103.0
38	92018.5
39	96934.0
40	96934.0
41	99755.0
42	102576.0
43	108307.0
44	114038.0
45	122231.5
46	130425.0
47	130425.0
48	167783.0
49	205141.0
50	190565.5
51	175990.0
52	159260.0
53	142530.0
54	142530.0
55	127886.5
56	113243.0
57	96577.0
58	79911.0
59	71834.5
60	63758.0
61	63758.0
62	56172.5
63	48587.0
64	42515.5
65	36444.0
66	31154.5
67	25865.0
68	25865.0
69	22198.0
70	18531.0
71	15642.0
72	12753.0
73	10429.5
74	8106.0
75	8106.0
76	6485.0
77	4864.0
78	3752.5
79	2641.0
80	2067.0
81	1493.0
82	1493.0
83	1109.5
84	726.0
85	560.5
86	395.0
87	286.5
88	178.0
89	178.0
90	143.5
91	109.0
92	73.0
93	37.0
94	27.5
95	18.0
96	18.0
97	11.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1928391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.4605257189963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04879676269465	44.68420606307433
2	10.645011977241795	11.594658971312008
3	3.101991137071681	5.068082043017726
4	1.2368947858812938	2.694477611927225
5	0.6793386371963985	1.849856966147118
6	0.42286964972699853	1.3817822060844094
7	0.2770444389290608	1.056159005412071
8	0.20852231498686002	0.9084987918661231
9	0.157339980865247	0.7711936267084355
>10	1.0626062176049043	11.064863846345341
>50	0.09600460169027769	3.5888919787033196
>100	0.05434806401427479	5.5370992437800854
>500	0.004758470152886931	1.8014987017612374
>1k	0.003901945525367284	4.063297477472113
>5k	2.8550820917321593E-4	1.0247779599293163
>10k+	2.8550820917321593E-4	2.9106555064591624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21027	1.090390901015406	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19295	1.00057509083998	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15836	0.8212027540058007	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8105	0.42029858052645963	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6342	0.32887521254766283	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5325	0.27613694525643395	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4937	0.2560165443626318	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4824	0.2501567368858286	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4635	0.2403558199556003	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4252	0.22049470257847087	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2987	0.15489597286027576	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2926	0.15173271395686871	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2830	0.14675447043675272	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2747	0.14245036405998576	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2345	0.12160396931950004	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2287	0.11859628052609662	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2245	0.11641829898604587	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.1058395315057994	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.10220956227238148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.1856703334541594E-5	0.0	0.0	0.0	0.0
7	5.1856703334541594E-5	0.0	0.0	5.1856703334541594E-5	0.0
8	5.1856703334541594E-5	0.0	0.0	5.1856703334541594E-5	0.0
9	5.1856703334541594E-5	0.0	0.0	1.5557011000362478E-4	0.0
10	5.1856703334541594E-5	0.0	0.0	2.59283516672708E-4	0.0
11	5.1856703334541594E-5	0.0	0.0	4.667103300108744E-4	0.0
12	5.1856703334541594E-5	0.0	0.0	6.741371433490408E-4	0.0
13	5.1856703334541594E-5	0.0	0.0	7.259938466835824E-4	0.0
14	5.1856703334541594E-5	0.0	0.0	8.815639566872071E-4	0.0
15	5.1856703334541594E-5	0.0	0.0	0.001140847473359915	0.0
16	5.1856703334541594E-5	0.0	0.0	0.0018149846167089558	0.0
17	5.1856703334541594E-5	0.0	0.0	0.002696548573396163	0.0
18	5.1856703334541594E-5	0.0	0.0	0.00357811253008337	0.0
19	5.1856703334541594E-5	0.0	0.0	0.004459676486770577	0.0
20	5.1856703334541594E-5	0.0	0.0	0.007208081763501282	0.0
21	5.1856703334541594E-5	0.0	0.0	0.012860462426966316	5.1856703334541594E-5
22	5.1856703334541594E-5	0.0	0.0	0.022246525730518343	5.1856703334541594E-5
23	5.1856703334541594E-5	0.0	0.0	0.02758776617397613	5.1856703334541594E-5
24	5.1856703334541594E-5	0.0	0.0	0.0370256861808627	5.1856703334541594E-5
25	5.1856703334541594E-5	0.0	0.0	0.04298920706433498	5.1856703334541594E-5
26	5.1856703334541594E-5	0.0	0.0	0.05356797454458147	5.1856703334541594E-5
27	5.1856703334541594E-5	0.0	0.0	0.11356618030264609	5.1856703334541594E-5
28	5.1856703334541594E-5	0.0	0.0	0.23667399401884784	5.1856703334541594E-5
29	5.1856703334541594E-5	0.0	0.0	0.3873177172056912	5.1856703334541594E-5
30	1.0371340666908319E-4	0.0	0.0	0.6056344382441112	5.1856703334541594E-5
31	1.0371340666908319E-4	0.0	0.0	0.8485830933664387	5.1856703334541594E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6115	0.0	24.051512	1
GTATTGG	790	0.0	18.734177	1
ATTGGAC	880	0.0	17.659092	3
TTGGACC	1080	0.0	17.300926	4
GAACCGT	65	0.0015804586	17.076925	6
GGACCCT	1105	0.0	16.407238	6
GTATTAG	420	0.0	16.297619	1
TGGACCC	1205	0.0	15.9668045	5
GACCCTC	1175	0.0	15.902128	7
CTAATAC	485	0.0	15.257732	3
TGCGACG	85	5.366494E-4	15.235293	22
TATACTG	305	0.0	15.163934	5
CCGTATA	135	3.9759107E-7	15.074075	2
GTATCAA	9760	0.0	15.050204	2
TATTGGA	1030	0.0	14.907766	2
GTCTAGG	100	1.0939744E-4	14.799999	1
CGCGATA	75	0.0041057486	14.799999	14
TATCGCC	75	0.0041057486	14.799999	33
TATACGT	140	6.0004095E-7	14.535715	4
AATACTG	490	0.0	14.346938	5
>>END_MODULE