FastQCFastQC Report
Wed 25 May 2016
SRR2088677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811488
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82621.018129658109547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76520.9429591072203163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59220.7297704956820064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31890.3929817816159943No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17330.21355830277219132No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG17280.21294215071572223No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA16070.1980312709491699No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14540.17917701802121533No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG13470.16599136401277653No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11710.1443028116250641No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA9440.11632950826136679No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.11238613509996451No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC8960.11041444851926337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.025.5032831
TGTCGGG502.7011527E-422.223
TAGCGAA450.003824853320.55555510
ATTCCGC555.1413017E-420.1818186
ATTTCGG759.260266E-619.73333429
GGTCGCA500.007033141318.515
CCGGTCT609.233624E-418.59
GCGCTTT500.007033141318.513
CTAATAC1409.458745E-1118.53
CGGTTGG752.0663226E-417.26666633
CCCGAAT803.3810321E-416.187513
GTATCAA36550.015.9439122
TAACGAA700.002591612715.8571434
AGTACCG700.002591612715.8571435
GGTAGCG700.002591612715.8571438
CTATAAG957.0555834E-515.5789481
TTTCGGT855.3617376E-415.23529430
TCTAGAC1252.955965E-614.8000013
TACACTG1252.955965E-614.8000015
TAGACCC750.00410302314.85
Produced by FastQC (version 0.11.5)