##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	811488
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62893721164084	31.0	31.0	34.0	30.0	34.0
2	31.84053738317757	31.0	31.0	34.0	30.0	34.0
3	31.92132847312591	31.0	31.0	34.0	30.0	34.0
4	35.6311146930084	37.0	35.0	37.0	33.0	37.0
5	35.50409494656729	37.0	35.0	37.0	33.0	37.0
6	35.549465919397456	37.0	35.0	37.0	33.0	37.0
7	35.419001882960686	37.0	35.0	37.0	33.0	37.0
8	35.44717481959068	37.0	35.0	37.0	33.0	37.0
9	37.05050105485232	39.0	37.0	39.0	33.0	39.0
10	36.866845843684686	39.0	37.0	39.0	33.0	39.0
11	36.95993409637604	39.0	37.0	39.0	33.0	39.0
12	36.814389122205135	39.0	37.0	39.0	32.0	39.0
13	36.8523601088371	39.0	37.0	39.0	32.0	39.0
14	37.85666208249537	40.0	37.0	41.0	32.0	41.0
15	37.867217999526794	40.0	37.0	41.0	33.0	41.0
16	37.76081716550337	40.0	37.0	41.0	32.0	41.0
17	37.78269549272448	40.0	37.0	41.0	32.0	41.0
18	37.743870519342245	40.0	37.0	41.0	32.0	41.0
19	37.78304300248433	40.0	37.0	41.0	32.0	41.0
20	37.666704868094165	40.0	37.0	41.0	32.0	41.0
21	37.601915247052325	39.0	37.0	41.0	32.0	41.0
22	37.46498038171852	39.0	36.0	41.0	32.0	41.0
23	37.29642705745495	39.0	36.0	41.0	31.0	41.0
24	37.155882773374344	39.0	36.0	41.0	31.0	41.0
25	37.33967846721085	39.0	36.0	41.0	31.0	41.0
26	37.222639151780434	39.0	36.0	41.0	31.0	41.0
27	37.12213982215387	39.0	36.0	41.0	31.0	41.0
28	36.95745839741315	39.0	36.0	41.0	31.0	41.0
29	36.859036732521	39.0	35.0	41.0	30.0	41.0
30	36.67175854726133	39.0	35.0	40.0	30.0	41.0
31	36.53166035726961	39.0	35.0	40.0	30.0	41.0
32	36.36559259040183	38.0	35.0	40.0	30.0	41.0
33	36.23162388106787	38.0	35.0	40.0	30.0	41.0
34	36.06591225008873	38.0	35.0	40.0	29.0	41.0
35	35.93598426594109	38.0	35.0	40.0	29.0	41.0
36	35.75553551007532	38.0	35.0	40.0	27.0	41.0
37	35.69624812689775	38.0	35.0	40.0	27.0	41.0
38	35.528721311960254	38.0	34.0	40.0	26.0	41.0
39	35.439233851886904	38.0	34.0	40.0	26.0	41.0
40	35.286901346661935	38.0	34.0	40.0	25.0	41.0
41	35.160664113332544	38.0	34.0	40.0	24.0	41.0
42	34.93583638944753	38.0	34.0	40.0	24.0	41.0
43	34.58131481919634	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	15.0
17	18.0
18	51.0
19	147.0
20	275.0
21	518.0
22	1036.0
23	1767.0
24	2860.0
25	4494.0
26	6492.0
27	9004.0
28	12362.0
29	16505.0
30	21285.0
31	26366.0
32	32514.0
33	40617.0
34	50083.0
35	61959.0
36	80065.0
37	111330.0
38	152720.0
39	178998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.140472810442056	21.329089277968375	13.854425450530384	23.67601246105919
2	18.97551165266769	23.11161717733349	34.86299242872353	23.049878741275286
3	19.5456987657242	24.35131511494933	32.56154126740014	23.54144485192634
4	13.965825742339998	16.583116447809456	36.19043041918057	33.26062739066998
5	12.757305098781499	38.399582002444895	35.11167139871446	13.73144150005915
6	32.45198943175993	37.37689281911747	14.040010449938878	16.13110729918372
7	26.14431759927442	32.93751725225758	21.78048227453764	19.13768287393036
8	26.675933593595964	34.694536456484876	19.343847352024923	19.285682597894237
9	24.78607200599393	14.613155092866437	21.707036949406522	38.89373595173311
10	16.284036239599352	27.903678181316298	34.03229622619188	21.779989352892464
11	33.06173350684175	22.72578275957254	23.383586695058952	20.828897038526755
12	20.699874797902126	27.335955676485668	31.06638668717221	20.89778283844
13	31.033114476122876	21.386514649631295	25.996687566544423	21.583683307701406
14	20.524025000985844	23.18358373752908	27.792031428684094	28.500359832800974
15	22.890665049883673	29.889782720138808	25.101048937260934	22.11850329271659
16	21.87660199534682	28.475590520130922	26.576486651681847	23.07132083284041
17	21.526134705627193	27.487529082377065	27.228129066603575	23.75820714539217
18	22.57963149177807	26.174016128396232	29.220395125990773	22.025957253834928
19	22.50199633266296	25.644495051066684	30.42337040104105	21.43013821522931
20	23.366211207066527	25.313621396742775	30.02484325091683	21.295324145273867
21	22.391581884143697	26.01948519263378	30.41819472376671	21.170738199455815
22	21.425208998777553	26.384000749240897	30.081406009700697	22.109384242280846
23	22.19268800031547	26.36292834890966	30.49546019164794	20.948923459126938
24	22.041607516069245	26.569832209471983	29.03826057809851	22.350299696360267
25	22.173094364919752	26.64389368665957	29.788487322055285	21.394524626365392
26	21.65540340707441	27.265837572459482	29.65601462991443	21.422744390551678
27	20.876463977286168	27.237494577861902	29.549790015379156	22.33625142947277
28	21.344862770613986	26.959117078749163	29.568028116250638	22.127992034386214
29	21.496066485271502	26.85930044560117	29.204621633345162	22.440011435782168
30	21.697178516503016	27.075076895776647	30.322937615836587	20.90480697188375
31	22.239885247841006	26.922024724949722	30.01473835719074	20.823351670018532
32	21.69372806498679	26.748269845025437	29.42384853503687	22.134153554950906
33	21.395510469655743	26.740876020347805	29.977399542568712	21.886213967427736
34	20.9516345281754	26.810747663551403	30.544998816988052	21.692618991285144
35	20.747564967072833	28.229745849599748	29.62508379667968	21.39760538664774
36	22.188621396742775	26.663980243700465	29.424218226270753	21.723180133286014
37	21.422251468906502	27.08259395086557	29.664147837059822	21.831006743168103
38	21.281768800031546	25.993360345439488	30.739826097243583	21.985044757285383
39	20.843807918293308	25.850536298749947	31.385306991600615	21.920348791356126
40	20.119582791119523	25.999768326826768	31.23348712488663	22.64716175716708
41	20.98478350881344	25.29895697779881	31.24642631807248	22.46983319531527
42	19.92561812374305	26.852399542568712	30.67944319570961	22.542539137978626
43	20.072262313182694	26.658558105603536	30.265881935407545	23.003297645806224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	261.0
2	221.0
3	410.5
4	600.0
5	600.0
6	904.5
7	1209.0
8	1311.5
9	1414.0
10	2249.0
11	3084.0
12	3084.0
13	5677.0
14	8270.0
15	12851.5
16	17433.0
17	16362.0
18	15291.0
19	15291.0
20	16735.5
21	18180.0
22	15212.0
23	12244.0
24	13989.0
25	15734.0
26	15734.0
27	17381.5
28	19029.0
29	20826.5
30	22624.0
31	25214.5
32	27805.0
33	27805.0
34	30597.0
35	33389.0
36	36155.5
37	38922.0
38	40766.0
39	42610.0
40	42610.0
41	43857.5
42	45105.0
43	47155.5
44	49206.0
45	51665.5
46	54125.0
47	54125.0
48	65675.5
49	77226.0
50	72402.5
51	67579.0
52	61954.0
53	56329.0
54	56329.0
55	51578.0
56	46827.0
57	41438.0
58	36049.0
59	32491.5
60	28934.0
61	28934.0
62	25445.0
63	21956.0
64	19257.0
65	16558.0
66	14069.0
67	11580.0
68	11580.0
69	9829.5
70	8079.0
71	6771.5
72	5464.0
73	4470.5
74	3477.0
75	3477.0
76	2837.5
77	2198.0
78	1668.5
79	1139.0
80	878.5
81	618.0
82	618.0
83	489.0
84	360.0
85	260.5
86	161.0
87	126.0
88	91.0
89	91.0
90	65.5
91	40.0
92	28.0
93	16.0
94	11.0
95	6.0
96	6.0
97	4.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	811488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.43003468490897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.73293655070624	54.38043139050397
2	8.448227637588346	10.717427441564706
3	2.2905220733291562	4.35863683673454
4	1.029180211520439	2.611237460550535
5	0.5948485255830104	1.8865631304998651
6	0.36502649858848474	1.3892186079827076
7	0.2521713592371471	1.1196666644067017
8	0.19838952712917082	1.0067083669540795
9	0.15364877808706995	0.8771352591021054
>10	0.8406613154226049	9.79739594722436
>50	0.058700398881764336	2.584692692101994
>100	0.030811833781222087	3.769757142775354
>500	0.0029251740871874417	1.2874272311808796
>1k	0.0013650812406874728	1.5126775182658512
>5k	5.850348174374884E-4	2.701024310152353
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8262	1.018129658109547	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7652	0.9429591072203163	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5922	0.7297704956820064	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3189	0.3929817816159943	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1733	0.21355830277219132	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1728	0.21294215071572223	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1607	0.1980312709491699	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1454	0.17917701802121533	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1347	0.16599136401277653	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1171	0.1443028116250641	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	944	0.11632950826136679	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.11238613509996451	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	896	0.11041444851926337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.4646082258764144E-4	0.0
12	0.0	0.0	0.0	7.393824677629244E-4	0.0
13	0.0	0.0	0.0	9.858432903505658E-4	0.0
14	0.0	0.0	0.0	0.0011090737016443866	0.0
15	0.0	0.0	0.0	0.0011090737016443866	0.0
16	0.0	0.0	0.0	0.0014787649355258489	0.0
17	0.0	0.0	0.0	0.0020949169919949524	0.0
18	1.2323041129382072E-4	0.0	0.0	0.0025878386371702353	0.0
19	1.2323041129382072E-4	0.0	0.0	0.0035736819275208015	0.0
20	1.2323041129382072E-4	0.0	0.0	0.005422138096928112	0.0
21	1.2323041129382072E-4	0.0	0.0	0.008009976734098348	0.0
22	1.2323041129382072E-4	0.0	0.0	0.01330888441973264	0.0
23	1.2323041129382072E-4	0.0	0.0	0.017375487992428724	0.0
24	1.2323041129382072E-4	0.0	0.0	0.022797626089356836	0.0
25	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.028835916242754053	0.0
26	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.03857111873496589	0.0
27	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.09772171615599984	0.0
28	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.22008951457076384	0.0
29	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.37991935801884935	0.0
30	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.5869464489924682	0.0
31	1.2323041129382072E-4	0.0	1.2323041129382072E-4	0.8573139713711109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2285	0.0	25.503283	1
TGTCGGG	50	2.7011527E-4	22.2	23
TAGCGAA	45	0.0038248533	20.555555	10
ATTCCGC	55	5.1413017E-4	20.181818	6
ATTTCGG	75	9.260266E-6	19.733334	29
GGTCGCA	50	0.0070331413	18.5	15
CCGGTCT	60	9.233624E-4	18.5	9
GCGCTTT	50	0.0070331413	18.5	13
CTAATAC	140	9.458745E-11	18.5	3
CGGTTGG	75	2.0663226E-4	17.266666	33
CCCGAAT	80	3.3810321E-4	16.1875	13
GTATCAA	3655	0.0	15.943912	2
TAACGAA	70	0.0025916127	15.857143	4
AGTACCG	70	0.0025916127	15.857143	5
GGTAGCG	70	0.0025916127	15.857143	8
CTATAAG	95	7.0555834E-5	15.578948	1
TTTCGGT	85	5.3617376E-4	15.235294	30
TCTAGAC	125	2.955965E-6	14.800001	3
TACACTG	125	2.955965E-6	14.800001	5
TAGACCC	75	0.004103023	14.8	5
>>END_MODULE