FastQCFastQC Report
Wed 25 May 2016
SRR2088676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374971
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147751.0745681181639468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140751.0236579535132013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105140.7646706730541953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56370.409972283051788No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32580.23695045204589768No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG30830.22422291088321136No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA29120.2117862849471007No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA26510.19280406641303707No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG25170.18305840632275153No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21960.15971245939005257No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA18000.1309118519590595No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16760.12189347993521318No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA15800.11491151449739667No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.11207509103828373No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15240.11083870132533703No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14180.10312944782108133No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.10174760049484681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49650.022.4682791
CGCTTAT656.903389E-519.9230771
GTATTGG3000.018.51
ACGTTTA1054.798949E-717.6190526
TCGCATA650.001580147117.07692323
TAGGTCG1107.8103403E-716.81818221
CGATCGT803.3831687E-416.187526
TTGGACC5750.015.4434794
GGACCCT5250.015.1523826
GTATCAA74900.014.9185572
ATTGGAC4000.014.83
GGCGATA750.004104950514.835
CGATAGA750.004104950514.837
ACGTTCG1252.959583E-614.79999922
GACCCTC5400.014.7314827
CTGTGCG1651.6454578E-814.5757599
CATTGCG1152.2103577E-514.4782629
GCCCTAG908.275473E-414.3888881
AGTGTAC908.275473E-414.3888888
GTGTATG2353.6379788E-1214.1702121
Produced by FastQC (version 0.11.5)