FastQCFastQC Report
Wed 25 May 2016
SRR2088675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432633
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT195241.3628054079446725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177341.237860638418911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141480.987552290084062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.530980369710875No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG50400.35179979799432237No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG42770.29854121746462636No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA41860.2921892766675066No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA39370.2748086914094538No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36490.25470584580977823No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG36270.25317021177091414No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA24520.17115339378612665No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC23700.16542966691399683No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA23220.1620791926473842No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21660.15119015128089328No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.14414019501156264No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.13394916911728266No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18780.13108730568121774No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG18390.128365045339595No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.12292052465634952No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC16230.11328791113983831No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15220.10623795487050766No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15020.10484192392608575No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14370.10030482335671453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.022.3629681
GTATTGG6200.019.6935481
TAGCGCG609.238333E-418.534
TGAACGA701.21946345E-418.55
CTAATAC2800.017.8392873
CTCTAAT2550.016.6862741
TAAACGT803.3832938E-416.18754
AACTGCG1201.9362142E-615.4166667
ACGTATA855.365296E-415.23529429
GTATCAA90000.015.23166752
TGGACCC8850.014.8418075
CTAGTAC2500.014.8000013
TAGGTCG1001.0936626E-414.821
TGTGCGC1508.104507E-814.810
AATGCGA750.004105062714.820
ATTGGAC7250.014.83
ACCGTCT750.004105062714.88
CCGCGCA750.004105062714.837
AATACTG3650.014.6986315
TAATACA2650.014.66037754
Produced by FastQC (version 0.11.5)