FastQCFastQC Report
Wed 25 May 2016
SRR2088674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197549
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133981.1187851186047502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124751.0417110281082445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95900.8008023053753959No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51090.4266213741567151No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG29650.24758903393514586No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27880.23280884540006294No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26380.22028326189575542No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG24200.20207941386949513No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA24050.20082685551906435No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22470.18763324089452707No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA16030.13385673571603335No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC15370.12834547897413803No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.12442079614278831No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14600.12191567944192681No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.11840851606072068No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12900.10772001813704492No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.1061334442264993No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG12020.10037167581451781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.021.0446111
ATTAGAG1502.5102054E-1017.2666663
GTATTGG4150.016.9397581
CGGTTGG1107.8082667E-716.81818233
TTGGACC6150.016.5447164
ATTGGAC4700.016.1382983
TTAACGG1403.4729965E-815.85714235
GTATTAG2350.015.7446811
TACACTG2300.015.2826095
CTAGACC855.3643854E-415.2352933
TAAGAGT855.3643854E-415.2352936
GTCTAGA1101.4518902E-515.1363641
AATCTAA1101.4518902E-515.13636421
TGGACCC6000.015.1083345
ATAGGCG750.004104539814.832
ATAGGAC750.004104539814.83
TGAACCG750.004104539814.85
GTCTAAC750.004104539814.831
CTAGTAC1508.1006874E-814.83
TGCGACG750.004104539814.822
Produced by FastQC (version 0.11.5)